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Synthesis of Infant Formula Fat Analogs Enriched with DHA from Extra Virgin Olive Oil and Tripalmitin

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

Structured lipids (SLs) containing palmitic, oleic, and docosahexaenoic acids for possible use in infant formulas were synthesized by enzymatic acidolysis reactions. The substrates used were tripalmitin, extra virgin olive oil free fatty acids (EVOOFFA), and docosahexaenoic acid single cell oil free fatty acids (DHASCOFFA) in 1:1:1, 1:2:1, 1:3:2, 1:4:2, and 1:5:1 molar ratios. Reactions were carried out at 65 °C for 24 h using Lipozyme® TL IM lipase. The products were analyzed for total and positional fatty acids by GC-FID, triacylglycerol (TAG) molecular species by HPLC-ELSD, and thermal behavior by DSC. The SLs, SL132, SL142, and SL151 had desirable fatty acid distribution for infant formula use with nearly 60 mol% palmitic acid at the sn-2 position and oleic acid predominantly at the sn-1,3 positions. The total DHA content of SL132, SL142, and SL151 were 7.54, 6.72, and 5.89 mol%, respectively. The major TAG molecular species in the SLs were PPP, OPO, and PPO. The melting completion temperature of SL132 was 37.1, 35.2 °C in SL142, and 32.9 °C in SL151. The SLs synthesized in this study have potential use in infant formulas.

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Acknowledgments

Research was supported by a grant from King Abdulaziz University (KAU) and KAU’s Vice President (Prof. Abdulrahaman O. Alyoubi).

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Authors and Affiliations

  1. Department of Food Science and Technology, University of Georgia, Athens, GA, 30602-2610, USA

    Garima Pande & Casimir C. Akoh

  2. Genomic and Biotechnology Section, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Kingdom of Saudi Arabia

    Jamal S. M. Sabir, Nabih A. Baeshen & Casimir C. Akoh

Authors
  1. Garima Pande
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  2. Jamal S. M. Sabir
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  3. Nabih A. Baeshen
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  4. Casimir C. Akoh
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Corresponding authors

Correspondence to Jamal S. M. Sabir, Nabih A. Baeshen or Casimir C. Akoh.

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Pande, G., Sabir, J.S.M., Baeshen, N.A. et al. Synthesis of Infant Formula Fat Analogs Enriched with DHA from Extra Virgin Olive Oil and Tripalmitin. J Am Oil Chem Soc 90, 1311–1318 (2013). https://doi.org/10.1007/s11746-013-2281-5

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  • DOI: https://doi.org/10.1007/s11746-013-2281-5

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