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Assessment of nutrient contents and bio-functional activities of edible fungus bio-fortified with copper, lithium and zinc

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Abstract

Bio-enrichment of edible mushrooms is an outstanding strategy to deliver essential nutrients to human. In this study, an edible fungus; Pleurotus pulmonarius was cultivated on spent mushroom substrate (SMS) supplemented with copper, lithium, and zinc. Proximate and mineral analysis of cultivated mushroom was determined using methods of AOAC. Antimicrobial activity of cultivated mushroom was assessed against microorganisms using agar well diffusion. Antioxidant property of mushroom was assessed against free radicals. Similar (p ≤ 0.05) protein contents of 18.93%, 18.80% and 17.90% were respectively obtained in P. pulmonarius biofortified with Cu + Li + Zn, Cu + Zn and Zn. Crude fibre in element fortified-mushroom ranged from 9.02 to 10.11%, while non-fortified mushroom was 8.66%. Copper content of P. pulmonarius fortified with Cu alone and Cu + Zn were 96.12 mg/100 g and 98.09 mg/100 g, respectively. Mushroom fortified with Zn has the highest zinc content of 520.15 mg/100 g. Mushroom fortified with Li and Li + Zn have a similar (p ≤ 0.05) Li content of 106.02 mg/100 g and 104.30 mg/100 g, respectively. Extract from mushroom-fortified with copper has the highest zone of inhibition (15.1 mm) against Klebsiella pneumoniae at 1.0 mg/ml. Mushroom fortified with Cu + Li + Zn and Li + Zn, respectively have similar (p ≤ 0.05) scavenging activities of 79.10 and 81.0% against DPPH. Mushroom fortified with Zn or Zn + Cu enhanced the growth of Lactobacillus acidophilus and Lactobacillus plantarum. Antimicrobial, antioxidant and prebiotic activities of fortified-mushroom could be attributed to arrays of phytochemicals and bio-accumulated elements. Hence, bio-fortified mushrooms can be used as functional foods and as biopharmaceuticals to treat ailments associated with nutrient deficient.

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  1. Department of Food Science and Technology, School of Agriculture, Food and Natural Resources, Olusegun Agagu University of Science and Technology, PMB 353, Okitipupa, Nigeria

    Clement Olusola Ogidi

  2. Department of Biological Sciences, Kings University, PMB 555, Odeomu, Nigeria

    Korede Opeyemi Oyebode

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Ogidi, C.O., Oyebode, K.O. Assessment of nutrient contents and bio-functional activities of edible fungus bio-fortified with copper, lithium and zinc. World J Microbiol Biotechnol 39, 56 (2023). https://doi.org/10.1007/s11274-022-03500-4

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