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The Influence of Cyclohexanone/Cyclohexanol on the Growth and Nitrogen Removal of Arthrospira platensis and the Cyclohexanone/Cyclohexanol Biotransformation

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Abstract

Arthrospira platensis is a candidate for removing nitrogen from caprolactam wastewater, but the concentrated cyclohexanone (CHN) and cyclohexanol (CHL) in the wastewater have an unknown effect on A. platensis growth. The aim of this study was to evaluate the feasibility of A. platensis for caprolactam wastewater treatment and obtain safe exposure concentrations of CHN and CHL. The biotransformation of CHN and CHL was investigated. Considering the changes of growth rate and pigment contents, the safe exposure concentrations of individual CHN and CHL were ≤ 800 and ≤ 400 mg L−1, respectively. For binary mixture, the safe exposure concentration was ≤ 200 + 100 mg L−1 CHN + CHL. Both chemicals induced decreased trichome length at all concentrations, but trichomes were large enough to be harvested efficiently by gravity filtration using filter membranes with a pore size of 25–40 μm under safe exposure. The nitrogen removal rate showed no difference in CHN and CHL treatment under safe exposure concentrations compared with the control, and the maximal nitrogen removal rate was 18.66 mg L−1 d−1. Besides, A. platensis catalyzed interconversion between CHN and CHL which preferred to convert CHN into CHL when chemicals coexisted. Results highlight the potential of A. platensis for the nitrogen removal from caprolactam wastewater when CHN and CHL were under the safe exposure concentrations.

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Funding

This work was supported by the SINOPEC Technology Development Program (Grant NO. 21807).

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Authors and Affiliations

  1. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, People’s Republic of China

    Caixia Liu, Yeguang Li & Xiaobin Wen

  2. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, 430074, People’s Republic of China

    Caixia Liu, Yeguang Li & Xiaobin Wen

  3. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Caixia Liu

  4. Research Center of Renewable Energy, SINOPEC Research Institute of Petroleum Processing, Beijing, 100083, People’s Republic of China

    Xu Li

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  1. Caixia Liu
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Liu, C., Li, X., Li, Y. et al. The Influence of Cyclohexanone/Cyclohexanol on the Growth and Nitrogen Removal of Arthrospira platensis and the Cyclohexanone/Cyclohexanol Biotransformation. Water Air Soil Pollut 231, 331 (2020). https://doi.org/10.1007/s11270-020-04672-1

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