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Effect of iron, zinc and manganese shortage-induced change on photosynthetic pigments, some osmoregulators and chlorophyll fluorescence parameters in lettuce

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Photosynthetica

Abstract

Although the beneficial role of Fe, Zn, and Mn on many physiological and biochemical processes is well established, effects of each of these elements on chlorophyll (Chl) a fluorescence and photosynthetic pigment contents is not well studied. The objective of this study was to evaluate effects of Fe, Zn, and Mn deficiency in two lettuce cultivars. The parameters investigated could serve also as physiological and biochemical markers in order to identify stress-tolerant cultivars. Our results indicated that microelement shortage significantly decreased contents of photosynthetic pigments in both lettuce cultivars. Chl a fluorescence parameters including maximal quantum yield of PSII photochemistry and performance index decreased under micronutrient deficiency, while relative variable fluorescence at J-step and minimal fluorescence yield of the dark-adapted state increased under such conditions in both cultivars. Micronutrient deficiency also reduced all parameters of quantum yield and specific energy fluxes excluding quantum yield of energy dissipation, quantum yield of reduction of end electron acceptors at the PSI, and total performance index for the photochemical activity. Osmoregulators, such as proline, soluble sugar, and total phenols were enhanced in plants grown under micronutrient deficiency. Fe, Zn, and Mn deficiency led to a lesser production of dry mass. The Fe deficiency was more destructive than that of Zn and Mn on the efficiency of PSII in both lettuce cultivars. Our results suggest that the leaf lettuce, which showed a higher efficiency of PSII, electron transport, quantum yield, specific energy fluxes, and osmoregulators under micronutrient deficiency, was more tolerant to stress conditions than crisphead lettuce.

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Abbreviations

Area:

area above the OJIP curve, it expresses the size of the reduced PQ pool

Chl:

chlorophyll

DM:

dry mass

ET0/RC:

electron transport flux per RC

FeD:

iron deficiency

FM:

fresh mass

Fm :

maximal fluorescence of the dark-adapted state

Fo :

minimal fluorescence yield of the dark-adapted state

Fv :

maximal variable fluorescence

Fv/Fm :

maximal quantum yield of PSII photochemistry

MnD:

manganese deficiency

PI:

performance index

PIabs :

performance index for the photochemical activity

PItot :

total performance index for the photochemical activity

RC:

reaction center

SM:

normalized area related to the number of electron carriers per electron transport chain

TChl:

total chlorophyll

TR0/RC:

trapped energy flux per RC

Vi :

relative variable fluorescence at time 30 ms I-step after start of actinic light pulse

Vj :

relative variable fluorescence at J-step

ZIP:

zinc transporters proteins

Φdo :

quantum yield of energy dissipation

φET20 :

quantum yield of electron transport from QA to QB in PSII

Φpo :

maximum quantum yield of primary PSII photochemistry

Φre10 :

quantum yield of reduction of end electron acceptors at the PSI

Ψo :

trapped exiton moves an electron in to the electron transport chain beyond QA

ZnD:

zinc deficiency.

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Authors and Affiliations

  1. Department of Horticultural Sciences, Faculty of Agriculture, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran

    H. R. Roosta & A. Estaji

  2. Department of Agronomy, Faculty of Agriculture, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran

    F. Niknam

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  1. H. R. Roosta
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  2. A. Estaji
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  3. F. Niknam
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Correspondence to A. Estaji.

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Roosta, H.R., Estaji, A. & Niknam, F. Effect of iron, zinc and manganese shortage-induced change on photosynthetic pigments, some osmoregulators and chlorophyll fluorescence parameters in lettuce. Photosynthetica 56, 606–615 (2018). https://doi.org/10.1007/s11099-017-0696-1

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  • DOI: https://doi.org/10.1007/s11099-017-0696-1

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