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Modulation in growth, photosynthetic efficiency, activity of antioxidants and mineral ions by foliar application of glycinebetaine on pea (Pisum sativum L.) under salt stress

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Abstract

A pot experiment was carried out to explore the role of glycinebetaine (GB) as foliar spray foliar on two pea (Pisum sativum L.) varieties (Pea 09 and Meteor Fsd) under saline and non-saline conditions. Thirty-two-day-old plants were subjected to two levels 0 and 150 mM of NaCl stress. Salt treatment was applied in full strength Hoagland’s nutrient solution. Three levels 0, 5 and 10 mM of GB were applied as foliar treatment on 34-day-old pea plants. After 2 weeks of foliar treatment with GB data for various growth and physiochemical attributes were recorded. Rooting-medium applied salt (150 mM NaCl) stress decreased growth, photosynthesis, chlorophyll, chlorophyll fluorescence and soluble protein contents, while increasing the activities of enzymatic (POD and CAT) and non-enzymatic (ascorbic acid and total phenolics) antioxidant enzymes. Foliar application of GB decreased root and shoot Na+ under saline conditions, while increasing shoot dry matter, root length, root fresh weight, stomatal conductance (g s), contents of seed ascorbic acid, leaf phenolics, and root and shoot Ca2+ contents. Of three GB (0, 5, 10 mM) levels, 10 mM proved to be more effective in mitigating the adverse effects of salinity stress. Overall, variety Pea 09 showed better performance in comparison to those of var. Meteor Fsd under both normal and salinity stress conditions. GB-induced modulation of seed ascorbic acid, leaf phenolics, g s, and root Ca2+ values might have contributed to the increased plant biomass, reduction of oxidative stress, increased osmotic adjustment and better photosynthetic performance of pea plants under salt stress.

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  1. Department of Botany, University of Agriculture, Faisalabad, Pakistan

    Nida Nusrat & Muhammad Shahbaz

  2. Department of Botany, Government College University, Faisalabad, Pakistan

    Shagufta Perveen

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  1. Nida Nusrat
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  2. Muhammad Shahbaz
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Correspondence to Muhammad Shahbaz.

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Communicated by L.A.Kleczkowski.

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Nusrat, N., Shahbaz, M. & Perveen, S. Modulation in growth, photosynthetic efficiency, activity of antioxidants and mineral ions by foliar application of glycinebetaine on pea (Pisum sativum L.) under salt stress. Acta Physiol Plant 36, 2985–2998 (2014). https://doi.org/10.1007/s11738-014-1670-1

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