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Anti-inflammatory and anti-oxidant activities of mesenchymal stem cells in chemically induced arthritic rats

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Abstract

Background

Mesenchymal stem cells (MSCs) have been extensively used as cell-based treatments for decades due to their anti-inflammatory, immunomodulatory, and healing abilities. The intent of our study was to determine the efficacy of MSCs in alleviating rheumatoid arthritis (RA) induced by Complete Freund's adjuvant (CFA) and to investigate the anti-inflammatory and antioxidant characteristics of MSCs.

Methods and results

Intrapedally injecting 0.1 ml of CFA directly into the footpad of the right hind paw daily for 2 days was used to induce RA. Arthritic rats received four doses of MSCs (1 × 106 cells/rat/dose) intravenously through the lateral tail vein. Our results showed that arthritic rats treated with MSCs exhibited reduced levels of paw edema. Furthermore, arthritic rats treated with MSCs exhibited a significant decrease in the levels of RF, CRP, IL-1β, TNF-α, IL-17 and ADAMTS-5, along with a significant increase in the levels of IL-4 and TIMP-3. Additionally, MSCs significantly reduced the expression of TGF-β. Both the glutathione (GSH) content and antioxidant activity of GST were enhanced by MSCs, while LPO levels were suppressed.

Conclusion

These findings provide further evidence that MSCs are valuable in treating RA, possibly due to their anti-inflammatory and anti-oxidative properties. Thus, MSCs have potential as a more effective therapeutic strategy for treating RA.

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Data availability

All data generated or analyzed during this study are included in the article.

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Funding

Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).

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Authors and Affiliations

  1. Molecular Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, P.O. Box 62521, Beni-Suef, Egypt

    Mohamed Y. Zaky & Eman E. Mohamed

  2. Department of Chemistry Department, Faculty of Science, Beni-Suef University, 62511, Beni-Suef, Egypt

    Rehab Mahmoud

  3. Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Beni-Suef University, 62511, Beni-Suef, Egypt

    Fatma Mohamed Halfaya

  4. Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, 62511, Beni-Suef, Egypt

    Ahmed Farghali

  5. Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, 62511, Beni-Suef, Egypt

    Fatma I. Abo El-Ela

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  1. Mohamed Y. Zaky
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  2. Eman E. Mohamed
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  6. Fatma I. Abo El-Ela
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Contributions

MYZ, RM, and AF contributed to the study conception and design. Material preparation, data collection and analysis were performed by EEM, MYZ, FMH, and FIAE. The manuscript was written and revised by MYZ and EEM. All authors read and approved the final manuscript.

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Correspondence to Mohamed Y. Zaky.

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All experiments using animals in this work were authorized by the Animal Ethics Committee of the Faculty of Science at Beni Suef University in Egypt (approval number: 022-424).

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Zaky, M.Y., Mohamed, E.E., Mahmoud, R. et al. Anti-inflammatory and anti-oxidant activities of mesenchymal stem cells in chemically induced arthritic rats. Mol Biol Rep 50, 9951–9961 (2023). https://doi.org/10.1007/s11033-023-08905-4

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