Summary
In order to study the in vitro culture and expansion of bone marrow mesenchymal stem cells in rats (rMSCs) and the possibility of rMSCs differentiation into retinal neural cells, the bone marrow-derived cells in SD rats were isolated and cultured in vitro. The retinal neural cells in SD rats were cultured and the supernatants were collected to prepare conditioned medium. The cultured rMSCs were induced to differentiate by two steps. Immunofluorescence method and anti-nestin, anti-NeuN, anti-GFAP and anti-Thy1.1 antibodies were used to identify the cells derived from the rMSCs. The results showed that the in vitro cultured rMSCs grew well and expanded quickly. After induction with two conditioned media, rMSCs was induced to differentiate into neural progenitor cells, then into retinal neural-like cells which were positive for nestin, NeuN, GFAP and Thy1.1 detected by fluorescence method. The findings suggested that rMSCs could be culture and expanded in vitro, and induced to differentiate into retinal neural-like cells.
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References
Pittenger M F, Mackay A M, Beck S C et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999,284:143–146
Jiang Y, Jahagirdar B N, Reinhardt R T et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002,418:41–49
Woodbury D, Schwarz E J, Prockop D J et al. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res, 2000,61:364–370
Tropepe V, Brenda L K, Bernard J C et al. Retinal stem cells in the adult mammalian eye. Science, 2000,287:2032–2036
Van Hoffelen S J, Young M J. Incorporation of murine brain progenitor cells into the developing mammalian retina. Invest Ophthalmol Vis Sci, 2003,44:426
Joanna E, Emanuela B, Diane S et al. Plasticity of bone marrow-derived stem cells. Stem Cells, 2004,22(4):487–500
Anthony K, Wei-Yong S, Ann S W et al. Differentiation of marrow stromal cells into photoreceptors in the rat eye. Neuroscience, 2003,23(21):7742–7749
Andreas H, Regina G, Stefan L et al. Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells. J Cell Science, 2004,117:4411–4422
Shiou S H, Kao C L, Peng C H et al. A novel in vitro differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells. Science, 2005,326:578–585
Ciccolini F, Svendsen C N. Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouse striatal precursor cells: Identification of neural precursor responding to both EGF and FGF-2. Neuroscience, 1998,18(19):7869–7880
Wu W, Wang K, Chen J H et al. Directional guidance of neuronal migration in the olfactory system by the protein slit. Nature, 1999,400:331–336
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This project was supported by a grant from National Natural Sciences Foundation of China (No. 30400488).
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Sun, X., Jiang, H. & Yang, H. In vitro culture of bone marrow mesenchymal stem cells in rats and differentiation into retinal neural-like cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 27, 598–600 (2007). https://doi.org/10.1007/s11596-007-0531-1
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DOI: https://doi.org/10.1007/s11596-007-0531-1