Abstract
Rapid gonad development is an important concern in the Macrobrachium nipponense aquaculture industry. The process of sexual differentiation and development is influenced by environmental factors, including water temperature and illumination time. In this study, we aimed to identify the key metabolites and genes from the androgenic gland, in response to different water temperatures and illumination times based on the integrated metabolomic and transcriptomic analysis of the androgenic gland during the reproductive vs. non-reproductive seasons. Histological observations revealed that the division of the androgenic gland in the reproductive season is more active than that in the non-reproductive season. The metabolic profiling analysis revealed that glycerophospholipid metabolism and sphingolipid metabolism represented the most enriched metabolic pathways of differentially expressed metabolites (DEMs). Transcriptome profiling analysis revealed that phagosome, spliceosome, RNA degradation, ribosome biogenesis in eukaryotes, and oxidative phosphorylation represented the main enriched metabolic pathways of differentially expressed genes (DEGs). A total of 14 DEGs from these metabolic pathways were considered as strong candidate genes involved in the male sexual development in M. nipponense, based on the expression difference between the reproductive vs. non-reproductive seasons. qPCR verifications of these 14 DEGs indicate the accuracy of the RNA-Seq. In addition, eight genes out of 14 DEGs showed high expression levels in the testis or/and androgenic gland. Predicting these eight genes may play an essential role in male sexual development of M. nipponense. The results of this study contribute to our knowledge of sexual development in male M. nipponense, as well as other crustacean species.
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Data availability
The reads of M. nipponense transcriptome were submitted to NCBI with the accession number of SRX5805440-SRX5805445. The data of M. nipponense were submitted to MetaboLights with the accession number of MTBLS1025.
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Not applicable.
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Funding
This research was supported by grants from the National Key R&D Program of China (2018YFD0900201); Central Public-interest Scientific Institution Basal Research Fund CAFS (2021JBFM02; 2020TD36); Jiangsu Agricultural Industry Technology System; the China Agriculture Research System-48 (CARS-48); the New cultivar breeding Major Project of Jiangsu province (PZCZ201745). Thank you for the Jiangsu Province Platform for the Conservation and Utilization of Agricultural Germplasm.
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S.J. designed and wrote the manuscript. W.Z. performed the qPCR analysis. S.J. and Y.X. provided the experimental prawns. H.Q. selected the differentially expressed metabolites. Y.G. selected the differentially expressed genes. Y.W. performed the histological observations. H.F. supervised the experiment.
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Permission was obtained from the Tai Lake Fishery Management Council and the committee of Freshwater Fisheries Research Center during the experimental programs. All experiments were performed in accordance with relevant guidelines and regulations.
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Jin, S., Zhang, W., Xiong, Y. et al. Genetic regulation of male sexual development in the oriental river prawn Macrobrachium nipponense during reproductive vs. non-reproductive season. Aquacult Int 30, 2059–2079 (2022). https://doi.org/10.1007/s10499-022-00887-7
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DOI: https://doi.org/10.1007/s10499-022-00887-7