Abstract
Simultaneous enantioseparation and rapid determination method of atenolol (AN) and amlodipine besylate (AMB) was firstly established using diphenylmethane diisocyanate-β-CD-modified COF (MDI-β-CD-modified COF) as chiral solid phases (CSP) by capillary electrochromatography (CEC). The optimal separation conditions were explored and the concentration of the acetate buffer solution was 20 mmol/L, pH = 5.0, operating voltage 20 kV, column temperature 20 °C, and then the four enantiomers of AN and AMB were baseline separated less than 5 min. The method validation was assessed by applying standard addition technique. The results showed that the concentration of each enantiomer exhibited a good linear relationship with the chromatographic peak area (r2 > 0.9918); the limit of detection (LOD) was 0.55–0.98 mg/L; the limit of quantification (LQD) was 1.83–3.27 mg/L; the average recoveries were in the range of 95.4–103.0%; the relative standard deviation (RSD) was less than 3.8%. The method established is rapid, simple, sensitive, accurate and reliable, and it can be used for routine analysis and quality control of the two chiral drugs separately or in combinations.
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The present work was partially supported by Young Creative Program of the Fundamental Research Funds in Heilongjiang Provincial Universities (2020), Grant/Award Number: No. 135509206.
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Gao, L., Xing, Z., Zhang, S. et al. Simultaneous Enantioseparation and Rapid Determination of Atenolol and Amlodipine Besylate by Capillary Electrochromatography. Chromatographia 85, 373–382 (2022). https://doi.org/10.1007/s10337-022-04141-3
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DOI: https://doi.org/10.1007/s10337-022-04141-3