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RP-HPLC Method for the Simultaneous Estimation of Eight Cardiovascular Drugs

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Abstract

The present study describes a convenient method for the separation and simultaneous determination of eight drugs used in cardiovascular diseases, viz., atenolol (ATN), lisinopril (LISI), hydrochlorothiazide (HCTZ), enalapril maleate (ENA), amlodipine besylate (AMLO), losartan potassium (LOSA), valsartan (VAL) and atorvastatin calcium (ATOR) in pharmaceutical formulations. A ZORBAX Rx-C8 column (250 × 4.6 mm, 5 μm particle size) was used with mobile phase consisting of acetonitrile, 10 mM dipotassium hydrogen phosphate buffer (pH 2.2 adjusted with orthophosphoric acid) using a gradient program and quantitative evaluation was performed at 210 nm with a flow rate of 1.0 mL min−1. Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with International Conference on Harmonization guidelines. The method is selective, precise, robust, accurate and can be used for routine analysis of 15 combination pharmaceutical formulations in quality control.

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Acknowledgments

The authors wish to thank the management of United States Pharmacopeia-India Private Limited group, for supporting this work. The authors are obligated to NIPER-Hyderabad, for providing the support and encouragement to carry out this work.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, 500037, India

    Chiguru Vishnuvardhan & N. Satheeshkumar

  2. Research and Development Laboratory, United States Pharmacopeia-India Private Limited, ICICI Knowledge Park, Turkapally, Shameerpet, Hyderabad, India

    P. Radhakrishnanand & Sameer G. Navalgund

  3. Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, 500037, India

    Krishnam Raju Atcha

Authors
  1. Chiguru Vishnuvardhan
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  2. P. Radhakrishnanand
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  3. Sameer G. Navalgund
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  4. Krishnam Raju Atcha
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  5. N. Satheeshkumar
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Corresponding author

Correspondence to N. Satheeshkumar.

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Vishnuvardhan, C., Radhakrishnanand, P., Navalgund, S.G. et al. RP-HPLC Method for the Simultaneous Estimation of Eight Cardiovascular Drugs. Chromatographia 77, 265–275 (2014). https://doi.org/10.1007/s10337-013-2598-0

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  • DOI: https://doi.org/10.1007/s10337-013-2598-0

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