Skip to main content
SpringerLink
Log in

First report of mango (Mangifera indica) stem-end rot caused by two Diaporthe species and their susceptibility to procymidone

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

In July 2013, a severe postharvest rot of mango fruits (cv. Lippens), with symptoms identical to stem-end rot caused by Lasiodiplodia theobromae or Neofusicoccum parvum, was found in Okinawa Prefecture, Japan. From affected fruit, Diaporthe species were consistently isolated. The isolates reproduced the original symptoms in healthy mango fruits after inoculation and were re-isolated from the inoculated fruits. Based on morphology and a phylogenetic analysis of rDNA-ITS, CAL, H3, EF1-α, and TUB, the isolates were classified into two Diaporthe species (Clade I and Clade II). In terms of susceptibility to procymidone, a registered fungicide against mango stem-end rot in Japan, the two Diaporthe species were less sensitive than previously reported mango stem-end rot pathogens and had MIC and EC50 values higher than the standard concentration applied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  Google Scholar 

  • Crous PW, Groenewald JZ, Risede J-M, Hywel-Jones NL (2004) Calonectria species and their Cylindrocladium anamorphs: species with sphaeropedunculate vesicles. Stud Mycol 50:415–430

    Google Scholar 

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Dissanayake AJ, Phillips AJL, Hyde KD, Yan JY, Li XH (2017) The current status of species in Diaporthe. Mycosphere 8:1106–1156

    Article  Google Scholar 

  • Fujimura M (2010) Mechanism of action of dicarboximide and phenylpyrrole on the stress-response signal transduction pathway (in Japanese). Jan J Pestic Sci 35:363–369

    Article  CAS  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    Article  CAS  Google Scholar 

  • Gomes RR, Glienke C, Videira SIR, Lombard L, Groenewald JZ, Crous PW (2013) Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia 31:1–41

    Article  CAS  Google Scholar 

  • Hisada Y, Kawase Y, Fujinami A (1983) Development of a new fungicide, procymidone (in Japanese with English summary). J Pesticide Sci 8:243–255

    Article  CAS  Google Scholar 

  • Horita H, Tanii A (1991) Occurrence of Botrytis cinerea resistant to dicarboximide fungicides in bean and vegetable fields of Tokachi district (in Japanese). Bull Hokkaido Pref Agric Exp Stn 63:53–60

    Google Scholar 

  • Huang F, Udayanga D, Wang X, Hou X, Mei X, Fu Y, Hyde KD, Li H (2015) Endophytic Diaporthe associated with Citrus: a phylogenetic reassessment with seven new species from China. Fungal Biol 119:331–347

    Article  Google Scholar 

  • Izumitsu K, Yoshimi A, Tanaka C (2007) Two-component response regulators ssk1p and skn7p additively regulate high-osmolarity adaptation and fungicide sensitivity in Cochliobolus heterostrophus. Eukaryot Cell 6:171–181

    Article  CAS  Google Scholar 

  • Johnson GI (1994) Stem-end rot. In: Ploetz RC, et al. (eds) Compendium of tropical fruit diseases. APS Press, St. Paul, pp 39–41

    Google Scholar 

  • Kayamori M, Mino K, Inagawa Y (2014) Dicarboximide resistance of Monilinia fructicola, the cause of brown rot of cherry, in Japan (in Japanese with English summary). Ann Rept Plant Prot North Jpn 65:98–100

    Google Scholar 

  • Kiso A, Nomura Y, Kanegae Y (1982) Control by certain fungicides of southern blight, Corticium rolfsii Curzi, on vegetables (in Japanese). Kyushu Pl Prot Res 28:66–69

    Article  CAS  Google Scholar 

  • Lim TH, Cha B (2003) Distribution of Monilinia fructicola isolates resistant to dicarboximide or to both procymidone and carbendazim in Korea. Plant Pathol J 19:46–50

    Article  Google Scholar 

  • Marques MW, Lima NB, de Morais MA, Michereff SJ, Phillips AJL, Câmara MPS (2013) Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil. Fungal Divers 61:195–208

    Article  Google Scholar 

  • Pitt WM, Sosnowski MR, Huang R, Qiu Y, Steel CC, Savocchia S (2012) Evaluation of fungicides for the management of Botryosphaeria canker of grapevines. Plant Dis 96:1303–1308

    Article  CAS  Google Scholar 

  • Punithalingam E (1974) New species of Phomopsis. Trans Brit Mycol Soc 63:229–236

    Article  Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  Google Scholar 

  • Serrato-Diaz LM, Rivera-Vargas LI, French-Monar RD (2014) First report of Diaporthe pseudomangiferae causing inflorescence rot, rachis canker and flower abortion of mango. Plant Dis 98:1004

    Article  CAS  Google Scholar 

  • Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (and other methods), version 4. Sinaur Associates, Sunderland

    Google Scholar 

  • Takushi T, Taba S, Moromizato Z, Sato T (2013) Stem-end rot of mango (Mangifera indica) caused by Lasiodiplodia theobromae sensu stricto (in Japanese with English summary). Jpn J Phytopathol 79:15–19

    Article  Google Scholar 

  • Takushi T, Arasaki C, Ajitomi A, Ooshiro A (2016) Stem-end rot of mango caused by Diaporthe pseudophoenicicola and Neofusicoccum parvum (abstract in Japanese). Jpn J Phytopathol 82:81

    Google Scholar 

  • Takushi T, Ajitomi A, Arasaki C, Ooshiro A, Sato T (2017) Stem-end rot of mango (Mangifera indica) caused by Neofusicoccum parvum in Japan (in Japanese with English summary). Jpn J Phytopathol 83:102–106

    Article  Google Scholar 

  • Takushi T, Ajitomi A, Arasaki C, Ooshiro A, Taba T (2018) Systematic application technique several effective fungicides for simultaneous control of stem-end rot and anthracnose disease of mango (in Japanese with English summary). Jpn J Trop Agric 11:43–52

    Google Scholar 

  • Udayanga D, Liu X, Crous PW, McKenzie EHC, Chukeatirote E, Hyde KD (2012) A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis). Fungal Divers 56:157–171

    Article  Google Scholar 

  • Udayanga D, Castlebury LA, Rossman AY, Hyde KD (2014) Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporellaD foeniculina and D rudis. Persoonia 32:83–101

    Article  CAS  Google Scholar 

  • Yonemoto Y (ed) (2008) Sin-tokusan-sirizu mango: the true of cultivation of ripened fruit (in Japanese). Noubunkyo, Tokyo, pp 37–44

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Snisky JJ, White TJ (eds) PCR protocols: a guide to methods and amplifications. Academic Press, San Diego, pp 315–322

    Google Scholar 

Download references

Acknowledgements

We thank Mr. M. Uechi (Okinawa Prefectural Department of Agriculture, Forestry and Fisheries, Agricultural Planning Division), Ms. M. Tokuyama (Okinawa Prefectural Department of Agriculture, Forestry and Fisheries, Miyako Agriculture, Forestry and Fisheries Promotion Center), Ms. S. Miyagi (Okinawa Prefectural Department of Agriculture, Forestry and Fisheries, Agricultural Support Division), and Mr. S. Tamashiro (Okinawa Prefectural Department of Agriculture, Forestry and Fisheries, Chubu Agricultural Development Center) for assistance in sample collection. We also thank Mr. M. Matsumura (Okinawa Prefectural Agricultural Research Center Nago Branch) for providing mango fruits for inoculation. This work was supported by Okinawa Special Promotion grant.

Author information

Authors and Affiliations

  1. Okinawa Prefectural Agricultural Research Center, Ishigaki Branch, 1178-6, Chisokobaru, Hirae, Ishigaki, Okinawa, 907-0003, Japan

    Atsushi Ajitomi

  2. Department of Clinical Plant Science, Faculty of Bioscience, Hosei University, Koganei, Tokyo, 184-8584, Japan

    Ayaka Minoshima, Hanh. H. Truong & Yuuri Hirooka

  3. Okinawa Prefectural Agricultural Research Center, 820 Makabe, Itoman, Okinawa, 901-0336, Japan

    Tetsuya Takushi & Maki Yamashiro

  4. Okinawa Prefectural Department of Agriculture, Forestry and Fisheries, Yaeyama Agriculture, Forestry and Fisheries Promotion Center, 438-1 Maezato, Ishigaki, Okinawa, 907-0002, Japan

    Atsushi Ooshiro

  5. Okinawa Prefectural Department of Agriculture, Forestry and Fisheries, Nanbu Agricultural Development Center, 517 Yamakawa, Haebaru, Okinawa, 901-1115, Japan

    Chiemi Arasaki

Authors
  1. Atsushi Ajitomi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ayaka Minoshima
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tetsuya Takushi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hanh. H. Truong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Atsushi Ooshiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Maki Yamashiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chiemi Arasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yuuri Hirooka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atsushi Ajitomi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ajitomi, A., Minoshima, A., Takushi, T. et al. First report of mango (Mangifera indica) stem-end rot caused by two Diaporthe species and their susceptibility to procymidone. J Gen Plant Pathol 86, 237–244 (2020). https://doi.org/10.1007/s10327-020-00921-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-020-00921-7

Keywords

Search

Navigation