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Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil

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Abstract

Members of the Botryosphaeriaceae are well known fungi associated with dieback, canker and fruit rot on various hosts worldwide, including mango. The aim of this study was identify a large collection of Botryosphaeriaceae species associated with dieback and stem-end rot of mango in the semi-arid region of Northeastern Brazil, and compare the species in relation to mycelial growth, pathogenicity and virulence. A total of 115 isolates were sampled and based on morphology and DNA sequence data (ITS and EF1-α) seven taxa were identified, namely, Botryosphaeria dothidea, B. mamane, Fusicoccum fabicercianum, Neofusicoccum parvum, N. brasiliense sp. nov, Neoscytalidium dimidiatum and Pseudofusicoccum stromaticum. B. dothidea and P. stromaticum were the most commonly isolated species, which represented 37 % and 33 % of all isolates respectively. B. mamane is reported for the first time in association with mango diseases worldwide. There were significant differences among the species obtained in this study in relation to optimum temperature for mycelial growth and mycelial growth rates. All species were pathogenic on mango fruit. There were significant differences in virulence among the species, with Ne. dimidiatum and N. parvum being the most virulent species, while P. stromaticum was the least virulent.

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Acknowledgments

We are grateful to Sequencing Platform LABCEN/CCB in the Universidade Federal de Pernambuco for use of its facilities. This work was financed by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 141275/2009-0) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/BEX 0245/12-7). M. P. S. Câmara, Marcos A. de Morais Junior and S. J. Michereff also acknowledge the CNPq research fellowship. A.J.L. Phillips thanks Fundação para a Ciência e a Tecnologia (Portugal) for financial support through grant PEst-OE/BIA/UI0457/2011

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  1. Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900, Recife, Brazil

    Marília W. Marques, Nelson B. Lima, Sami J. Michereff & Marcos P. S. Câmara

  2. Departamento de Genética, Universidade Federal de Pernambuco, 50732-970, Recife, Brazil

    Marcos A. de Morais Jr

  3. Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal

    Alan J. L. Phillips

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  1. Marília W. Marques
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  2. Nelson B. Lima
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Correspondence to Marcos P. S. Câmara.

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Marques, M.W., Lima, N.B., de Morais, M.A. et al. Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil. Fungal Diversity 61, 195–208 (2013). https://doi.org/10.1007/s13225-013-0258-1

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