Skip to main content
SpringerLink
Log in

A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

  • Published:
Fungal Diversity Aims and scope Submit manuscript

Abstract

The genus Diaporthe (Phomopsis) includes important plant pathogenic fungi with wide host ranges and geographic distributions. In the present study, phylogenetic species recognition in Diaporthe is re-evaluated using a multi-locus phylogeny based on a combined data matrix of rDNA ITS, and partial sequences from the translation elongation factor 1-α (EF 1-α), β tubulin (TUB) and calmodulin (CAL) molecular markers. DNA sequences of available ex-type cultures have been included, providing a multi-locus backbone tree for future studies on Diaporthe. Four utilizable loci were analyzed individually and in combination, and ITS, EF 1-α and multi-locus phylogenetic trees are presented. The phylogenetic tree inferred by combined analysis of four loci provided the best resolution for species as compared to single gene analysis. Notes are provided for nine species previously known in Phomopsis that are transferred to Diaporthe in the present study. The unraveling of cryptic species complexes of Diaporthe based on Genealogical Concordance Phylogenetic Species Recognition (GCPSR) is emphasized.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Alves A, Correia A, Phillips AJL (2006) Multi-gene genealogies and morphological data support Diplodia cupressi sp. nov., previously recognized as D. pinea f. sp. cupressi, as a distinct species. Fungal Divers 23:1–15

    Google Scholar 

  • Aoki T, O'Donell K, Scandiani MM (2005) Sudden death syndrome of soybean in South America is caused by four species of Fusarium: Fusarium brasiliense sp. nov., F. cuneirostrum sp. nov., F. tucumaniae, and F. virguliforme. Mycoscience 46:162

    Article  Google Scholar 

  • Avise JC, Arnold J, Ball RM, Bermingham E, Lamb T, Neigel JE, Reeb CA, Saunders NC (1987) Intraspecific Phylogeography: the Mitochondrial DNA Bridge between Population Genetics and Systematics. Ann Rev Ecol Syst 18:489–522

    Google Scholar 

  • Botella L, Diez JJ (2011) Phylogenic diversity of fungal endophytes in Spanish stands of Pinus halepensis. Fungal Divers 47:9–18

    Article  Google Scholar 

  • Cai L, Giraud T, Zhang N, Begerow D, Cai G, Shivas RG (2011a) The evolution of species concepts and species recognition criteria in plant pathogenic fungi. Fungal Divers 50:121–133

    Article  Google Scholar 

  • Cai L, Udayanga D, Manamgoda DS, Maharachchikumbura SSN, Liu XZ, Hyde KD (2011b) The need to carry out re-inventory of tropical plant pathogens. Trop Plant Pathol 36:205–213

    Article  Google Scholar 

  • Carbone I, Kohn L (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Castlebury L (2005) The Diaporthe vaccinii complex of fruit pathogens. Inoculum 56:12

    Google Scholar 

  • Castlebury LA, Farr DF, Rossman AY (2001) Phylogenetic distinction of Phomopsis isolates from cucurbits. Inoculum 52:25

    Google Scholar 

  • Castlebury LA, Farr DF, Rossman AY, Jaklitsch WJ (2003) Diaporthe angelicae comb. nov., a modern description and placement of Diaporthopsis in Diaporthe. Mycoscience 44:203–208

    Google Scholar 

  • Catalano V, Rekab D, Firrao G, Vannacci G, Vergara EM (2012) An endopolygalacturonase gene of Diaporthe helianthi. Phytopathol Mediterr 51:23–36

    Google Scholar 

  • Choi YW, Hyde KD, Ho WH (1999) Single spore isolation of fungi. Fungal Divers 3:29–38

    Google Scholar 

  • Chomnunti P, Schoch CL, Aguirre-Hudson B, Ko-Ko TW, Hongsanan S, Jones EBG, Kodsueb R, Phookamsak R, Chukeatirote E, Bahkali AH, Hyde KD (2011) Capnodiaceae. Fungal Divers 51:103–134

    Article  PubMed  Google Scholar 

  • Coetzee MPA, Wingfield BD, Bloomer P, Wingfield MJ (2005) Phylogenetic analyses of DNA sequences reveal species partitions amongst isolates of Armillaria from Africa. Mycol Res 109:1223–1234

    Article  PubMed  CAS  Google Scholar 

  • Cowley RB, Ash GJ, J Harper JDI, Luckett DJ (2012) Evaluation of resistance to Phomopsis stem blight (caused by Diaporthe toxica) in Lupinus albus. Eur J Plant Pathol. 133:631–644

  • Crouch JA, Clarke BB, Hillman BI (2009) What is the value of ITS sequence data in Colletotrichum systematics and species diagnosis? A case study using the falcate-spored graminicolous Colletotrichum group. Mycologia 101:648–656

    Article  PubMed  Google Scholar 

  • Crous PW (2005) Impact of molecular phylogenetics on the taxonomy and diagnostics of fungi. Bull OEPP/EPPO 35:47–51

    Google Scholar 

  • Crous PW, Groenewald JZ (2005) Hosts, species and genotypes: opinions versus data. Australas Plant Pathol 34:463–470

    Article  Google Scholar 

  • Crous PW, Gams W, Stalpers JA, Robert V, Stegehuis G (2004) MycoBank: an online initiative to launch mycology into the 21st century. Stud Mycol 50:19–22

    Google Scholar 

  • Crous PW, Groenewald JZ, Groenewald M, Caldwell P, Braun U, Harrington TC (2006) Species of Cercospora associated with grey leaf spot of maize. Stud Mycol 55:189–197

    Article  PubMed  Google Scholar 

  • Crous PW, Groenewald JZ, Shivas RG, Edwards J, Seifert KA et al (2011) Fungal planet description sheets: 69–91. Persoonia 26:108–156

    Article  PubMed  CAS  Google Scholar 

  • Damm U, Cannon PF, Woudenberg JHC, Johnston PR, Weir BS, Tan YP, Shivas RG, Crous PW (2012) The Colletotrichum boninense species complex. Stud Mycol 73:1–36

    Google Scholar 

  • de Guido MA, Pollastro S, Carlucci A, de Miccolis Angelini RM, Faretra F (2003) Phomopsis viticola is easily transformed with hph and Bmlr genes. J Plant Pathol 85:43–52

    Google Scholar 

  • Diogo ELF, Santos JM, Phillips AJL (2010) Phylogeny, morphology and pathogenicity of Diaporthe and Phomopsis species on almond in Portugal. Fungal Divers 44:107–115

    Article  Google Scholar 

  • Einax E, Voigt K (2003) Oligonucleotide primers for the universal amplification of β-tubulin genes facilitate phylogenetic analyses. Organ Divers Evol 3:185–194

    Article  Google Scholar 

  • Farr DF, Castlebury LA, Rossman AY, Putnam ML (2002b) A new species of Phomopsis causing twig dieback of Vaccinium vitis-idaea (lingonberry). Mycol Res 106:745–752

    Google Scholar 

  • Farr DF, Castlebury LA, Rossman AY (2002b) Morphological and molecular characterization of Phomopsis vaccinii and additional isolates of Phomopsis from blueberry and cranberry in the eastern United States. Mycologia 94:494–504

    Article  PubMed  CAS  Google Scholar 

  • Farris JS, Kallersjo M, Kluge AG, Bult C (1994) Testing significance of incongruence. Cladistics 10:315–320

    Article  Google Scholar 

  • Garcia-Guzman G, Morales E (2007) Life-history strategies of plant pathogens: distribution patterns and phylogenetic analysis. Ecology 88:589–596

    Article  PubMed  Google Scholar 

  • Garcia-Reyne A, López-Medrano F, Morales JM, García Esteban C, Martín I, Eraña I, Meije Y, Lalueza A, Alastruey-Izquierdo A, Rodríguez-Tudela JL, Aguado JM (2011) Cutaneous infection by Phomopsis longicolla in a renal transplant recipient from Guinea: first report of human infection by this fungus. Transpl Infect Dis 13:204–207

    Article  PubMed  CAS  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    PubMed  CAS  Google Scholar 

  • Glienke C, Pereira OL, Stringari D, Fabris J, Kava-Cordeiro V, Galli-Terasawa L, Cunnington J, Shivas RG, Groenewald JZ, Crous PW (2011) Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot. Persoonia 26:47–56

    Article  PubMed  CAS  Google Scholar 

  • Groenewald M, Groenewald JZ, Crous PW (2005) Distinct species exist within the Cercospora apii morphotype. Phytopathology 95:951–959

    Article  PubMed  CAS  Google Scholar 

  • Hawksworth DL (2011) A new dawn for the naming of fungi: impacts of decisions made in Melbourne in July 2011 on the future publication and regulation of fungal names. IMA Fungus 2:155–162

    Article  PubMed  Google Scholar 

  • Hofstetter V, Miadlikowska J, Kauff F, Lutzoni F (2007) Phylogenetic comparison of protein-coding versus ribosomal RNA-coding sequence data: a case study of the Lecanoromycetes (Ascomycota). Mol Phylogenet Evol 44:412–426

    Article  PubMed  CAS  Google Scholar 

  • Hudson RR, Coyne JA (2002) Mathematical consequences of the genealogical species concept. Evolution 56:1557–1565

    PubMed  Google Scholar 

  • Hyde KD, McKenzie EHC, KoKo TW (2011) Towards incorporating anamorphic fungi in a natural classification — checklist and notes for 2010. Mycosphere 2:1–88

    Google Scholar 

  • Iriart I, Binois R, Fior R, Blanchet R, Berry A, Cassaing S, Amazan E, Papot E, Carm B, Aznar C, Couppié P (2011) Eumycetoma caused by Diaporthe phaseolorum (Phomopsis phaseoli): a case report and a mini-review of Diaporthe/Phomopsis spp invasive infections in humans. Clin Microbiol Infec 17:1492–1494

    Article  CAS  Google Scholar 

  • James TY, Kauff F, Schoch CL, Matheny PB, Hofstetter V, Cox CJ, Celio G, Gueidan C, Fraker E, Miadlikowska J, Lumbsch HT, Rauhut A, Reeb V et al (2006) Reconstructing the early evolution of Fungi using a six-gene phylogeny. Nature 443:818–822

    Article  PubMed  CAS  Google Scholar 

  • Jiang SX, Ma HB (2010) A new species of Phomopsis on Castanea mollisima. Mycosystema 29:467–471

    CAS  Google Scholar 

  • Jurković D, Vrandečić K, Ćosić J, Riccioni L, Duvnjak T (2007) Morphological identification of Diaporthe/Phomopsis sp. isolated from Xanthium italicum. Poljoprivreda (Osijek) 13:10–14

  • Kanematsu S, Adachi Y, Ito T (2007) Mating-type loci of heterothallic Diaporthe spp. homologous genes are present in opposite mating-types. Curr Genet 52:11–22

    Article  PubMed  CAS  Google Scholar 

  • Katoh K, Toh H (2008) Recent developments in the MAFFT multiple sequence alignment program. Brief Bioinform 9:276–285

    Article  Google Scholar 

  • Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066

    Article  PubMed  CAS  Google Scholar 

  • Kishino H, Hasegawa M (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. J Mol Evol 29:170–179

    Article  PubMed  CAS  Google Scholar 

  • Lombard L, Crous PW, Wingfield BD, Wingfield MJ (2010) Phylogeny and systematics of the genus Calonectria. Stud Mycol 66:31–69

    Article  PubMed  CAS  Google Scholar 

  • Lumbsch HT, Schmitt I, Lindemuth R, Miller A, Mangold A, Fernandez F, Huhndorf S (2005) Performance of four ribosomal DNA regions to infer higher-level phylogenetic relationships of inoperculate euascomycetes (Leotiomyceta). Mol Phylogenet Evol 34:512–524

    Article  PubMed  CAS  Google Scholar 

  • Manamgoda DS, Cai L, Bahkali AH, Chukeatirote E, Hyde KD (2011) Cochliobolus: an overview and current status of species. Fungal Divers 51:3–42

    Article  Google Scholar 

  • McNeill J, Turland NJ, Monro A, Lepschi BJ (2011) XVIII International Botanical Congress: preliminary mail vote and report of Congress action on nomenclature proposals. Taxon 60:1507–1520

    Google Scholar 

  • Mejía LC, Castlebury LA, Rossman AY, Sogonov MV, White JF Jr (2011) A systematic account of the genus Plagiostoma (Gnomoniaceae, Diaporthales) based on morphology, host-associations, and a four-gene phylogeny. Stud Mycol 68:211–235

    Article  PubMed  Google Scholar 

  • Mejia LC, Rossman AY, Castlebury LA, White JF Jr (2011) New species, phylogeny, host-associations, and geographic distribution of the genus Cryptosporella (Gnomoniaceae, Diaporthales). Mycologia 103:379–399

    Article  PubMed  Google Scholar 

  • Moleleki N, Preisig O, Wingfield MJ, Crous PW, Wingfield BD (2002) PCR-RFLP and sequence data delineate three Diaporthe species associated with stone and pome fruit trees in South Africa. Eur J Plant Pathol 108:909–912

    Article  CAS  Google Scholar 

  • Mostert L, Crous PW, Kang JC, Phillips AJL (2001) Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia 93:146–167

    Article  Google Scholar 

  • Murali TS, Suryanarayanan TS, Geeta R (2006) Endophytic Phomopsis species: host range and implications for diversity estimates. Can J Microbiol 52:673–680

    Article  PubMed  CAS  Google Scholar 

  • Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  • Phillips AJL, Crous PW, Alves A (2007) Diplodia seriata, the anamorph of Botryosphaeria obtusa. Fungal Divers 25:141–155

    Google Scholar 

  • Phoulivong S, Cai L, Chen H, McKenzie EHC, Abdelsalam K, Chukeatirote E, Hyde KD (2010) Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Divers 44:33–43

    Article  Google Scholar 

  • Rehner SA, Uecker FA (1994) Nuclear ribosomal internal transcribed spacer phylogeny and host diversity in the coelomycetes Phomopsis. Can J Bot 72:166–167

    Article  Google Scholar 

  • Rocha ACS, Garcia D, Uetanabaro APT, Carneiro RTO, Isabela S, Araújo IS, Carlos Mattos CRR, Góes-Neto A (2011) Foliar endophytic fungi from Hevea brasiliensis and their antagonism on Microcyclus ulei. Fungal Divers 47:75–84

    Article  Google Scholar 

  • Rokas A, Williams BL, King N, Carroll SB (2003a) Genome scale approaches to resolving incongruence in molecular phylogenies. Nature 425:798–804

    Article  PubMed  CAS  Google Scholar 

  • Rokas A, King N, Finnerty JR, Carroll SB (2003b) Conflicting phylogenetic signals at the base of the metazoan tree. Dev Evol 5:346–359

    Article  CAS  Google Scholar 

  • Rossman AY, Farr DF, Castlebury LA (2007) A review of the phylogeny and biology of the Diaporthales. Mycoscience 48:135–144

    Article  Google Scholar 

  • Santos JM, Phillips AJL (2009) Resolving the complex of Diaporthe (Phomopsis) species occurring on Foeniculum vulgare in Portugal. Fungal Divers 34:111–125

    Google Scholar 

  • Santos JM, Correia VG, Phillips AJL (2010) Primers for mating-type diagnosis in Diaporthe and Phomopsis: their use in teleomorph induction in vitro and biological species definition. Fungal Biol 114:255–270

    Article  PubMed  CAS  Google Scholar 

  • Santos JM, Vrandečić K, Ćosić J, Duvnjak T, Phillips AJL (2011) Resolving the Diaporthe species occurring on soybean in Croatia. Persoonia 27:9–19

    Article  PubMed  CAS  Google Scholar 

  • Schmitt I, Crespo A, Divakar PK, Fankhauser JD, Herman-Sackett E, Kalb K, Nelsen MP, Nelson NA, Rivas-Plata E, Shimp AD, Widhelm T, Lumbsch HT (2009) New primers for promising single copy genes in fungal phylogenetics and systematic. Persoonia 23:35–40

    Article  PubMed  CAS  Google Scholar 

  • Schoch CL, Shoemaker RA, Seifert KA, Hambleton S, Spatafora JW, Crous PW (2006) A multigene phylogeny of the Dothideomycetes using four nuclear loci. Mycologia 98:1041–1052

    Article  PubMed  CAS  Google Scholar 

  • Schoch CL, Seifert KA, Huhndorf S et al (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. P Natl Acad Sci 109:6241–6246

    Article  CAS  Google Scholar 

  • Shivas RG, Cai L (2011) Cryptic fungal species unmasked. Microbiology Australia 33:36–37

    Google Scholar 

  • Summerell BA, Leslie JF (2011) Fifty years of Fusarium: how were nine species ever enough? Fungal Divers 50:135–144

    Article  Google Scholar 

  • Summerell BA, Laurence MH, Liew ECY, Leslie JF (2010) Biogeography and phylogeography of Fusarium: a review. Fungal Divers 44:3–13

    Article  Google Scholar 

  • Sun X, Guo LD, Hyde KD (2011) Community composition of endophytic fungi in Acer truncatum and their role in decomposition. Fungal Divers 47:85–95

    Article  Google Scholar 

  • Sun S, Van K, Kim MY, Min KH, Lee YW, Lee SH (2012) Diaporthe phaseolorum var. caulivora, a causal agent for both stem canker and seed decay on soybean. Plant Pathol J 28:55–59

    Article  Google Scholar 

  • Sutton DA, Timm WD, Morgan-Jones G, Rinaldi MG (1999) Human phaeohyphomycotic osteomyelitis caused by the coelomycete Phomopsis Saccardo 1905: criteria for identification, case history, and therapy. J Clin Microbiol 37:807–811

    PubMed  CAS  Google Scholar 

  • Swofford DL (2002) PAUP 4.0b10: phylogenetic analysis using parsimony. Sinauer Associates, Sunderland

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS, Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol 31:21–32

    Article  PubMed  CAS  Google Scholar 

  • Templeton AR (1989) The meaning of species and speciation: a genetic perspective. In: Otte D, Endler JA (eds) Speciation and its consequences. Sinauer, Sunderland, pp 3–27

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Thompson SM, Tan YP, Young AJ, Neate SM, Aitken EA, Shivas RG (2011) Stem cankers on sunflower (Helianthus annuus) in Australia reveal a complex of pathogenic Diaporthe (Phomopsis) species. Persoonia 27:80–89

    Article  PubMed  CAS  Google Scholar 

  • Udayanga D, Liu XX, McKenzie EHC, Chukeatirote E, Bahkali AH, Hyde KD (2011) The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Divers 50:189–225

    Article  Google Scholar 

  • Udayanga D, Liu XX, Crous PW, McKenzie EHC, Chukeatirote E, Hyde KD (2012) Multilocus phylogeny of Diaporthe reveals three new cryptic species from Thailand. Cryptogamie Mycologie. In Press

  • Uecker FA (1988) A world list of Phomopsis names with notes on nomenclature, morphology and biology. Mycol Mem 13:1–231

    Google Scholar 

  • van der Aa HA, Noordeloos ME, Gruyter J (1990) Species concepts in some larger genera of the coelomycetes. Stud Mycol 32:3–19

    Google Scholar 

  • van Niekerk JM, Groenewald JZ, Farr DF, Fourie PH, Halleen F, Crous PW (2005) Reassessment of Phomopsis species on grapevine. Australas Plant Path 34:27–39

    Article  Google Scholar 

  • van Rensburg JCJ, Lamprecht SC, Groenewald JZ, Castlebury LA, Crous PW (2006) Characterisation of Phomopsis spp. associated with die-back of rooibos (Aspalathus linearis) in South Africa. Stud Mycol 55:65–74

    Article  PubMed  Google Scholar 

  • van Warmelo KT, Marasas WFO, Adelaar TF, Kellerman TS, van Rensburg IBJ, Minne JA (1970) Experimental evidence that lupinosis of sheep is a mycotoxicosis caused by the fungus Phomopsis leptostromiformis (Kuhn) Bubak. J S Afr Vet Med Assoc 41:235–247

    Google Scholar 

  • Walker DM, Castlebury LA, Rossman AY, White JF (2012) New molecular markers for fungal phylogenetics: two genes for species level systematics in the Sordariomycetes (Ascomycota). Molecular Phylogenetics and Evolution. Accepted. Manuscript MPE-11-256

  • Wang XH, Chen GQ, Huang F, Zhang JZ, Hyde KD, Li HG (2011) Phyllosticta species associated with citrus diseases in China. Fungal Divers 52:209–224

    Article  Google Scholar 

  • Wehmeyer LE (1933) The genus Diaporthe Nitschke and its segregates. University of Michigan Studies Scientific Series 9:1–34

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. New York Academic Press. pp 315–322

    Google Scholar 

  • Wikee S, Udayanga D, Crous PW, McKenzie EHC, Chukeatirote E, Bahkali AH, Hyde KD (2011) Phyllostica- an overview of current status of species recognition. Fungal Divers 51:46–60

    Article  Google Scholar 

  • Wingfield MJ, de Beer ZW, Slippers B, Wingfield BD, Groenewald JZ, Lombard L, Crous PW (2012) One fungus one name promotes progressive plant pathology. Molecular Plant Pathology 13:604–613

    Google Scholar 

  • Wu ZH, Wang TH, Huang W, Qu YB (2001) A simplified method for chromosome DNA preparation from filamentous fungi. Mycosystema 20:575–577

    CAS  Google Scholar 

  • Zhang N (2002) Molecular phylogeny of Melanospora and Diaporthales, and population genetics of dogwood anthracnose fungus. PhD Thesis. Department of Biological Sciences, Lousiana State University

  • Zhang AW, Hartman GL, Riccioni L, Chen WD, Ma RZ, Pedersen WL (1997) Using PCR to distinguish Diaporthe phaseolorum and Phomopsis longicolla from other soybean fungal pathogens and to detect them in soybean tis-sues. Plant Dis 81:1143–1149

    Article  CAS  Google Scholar 

  • Zhang AW, Riccioni L, Pedersen WL, Kollipara KP, Hartman GL (1998) Molecular identification and phylogenetic grouping of Diaporthe phaseolorum and Phomopsis longicolla isolates from soybean. Phytopathology 88:1306–1314

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This project is supported by the State Key Laboratory of Mycology, Institute of Microbiology by grant NFSC Y2JJ011002 and Thailand Research fund BRG 52800002. Dhanushka Udayanga thanks the State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing and the Mushroom Research Foundation, Chiang Mai, Thailand for a postgraduate scholarship. Lei Cai (CAS-Beijing), Nilam F. Wulandari (Chiang Mai University, Thailand) and Samantha C. Karunarathna, Dimuthu S. Manamgoda (Mae Fah Luang University, Thailand) are thanked for providing fungal specimens

Author information

Authors and Affiliations

  1. Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Dhanushka Udayanga, Ekachai Chukeatirote & Kevin D. Hyde

  2. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Dhanushka Udayanga, Ekachai Chukeatirote & Kevin D. Hyde

  3. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No 3 1st West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China

    Dhanushka Udayanga & Xingzhong Liu

  4. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands

    Pedro W. Crous

  5. Landcare Research, Private Bag 92170, Auckland, New Zealand

    Eric H. C. McKenzie

Authors
  1. Dhanushka Udayanga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xingzhong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pedro W. Crous
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eric H. C. McKenzie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ekachai Chukeatirote
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kevin D. Hyde
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Dhanushka Udayanga or Xingzhong Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Udayanga, D., Liu, X., Crous, P.W. et al. A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis). Fungal Diversity 56, 157–171 (2012). https://doi.org/10.1007/s13225-012-0190-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13225-012-0190-9

Keywords

Search

Navigation