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An electrochemical aptasensor for the detection of chloramphenicol based on ultra-small Au-inserted hollow PCN-222 MOF

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Abstract

The excessive utilization of antibiotics has led to significant water contamination and posed severe threats to human well-being. Consequently, the pressing imperative to identify antibiotics in the environment arises. In this study, we have successfully synthesized a hollow PCN-222 MOF distinguished by its substantial surface area and abundant functional groups, particularly the porphyrin cores. To augment the electrical conductivity of the hollow PCN-222 (HPCN-222), gold (Au) particles were incorporated within the porphyrin core using a fundamental hydrothermal method. This modification facilitated the effective immobilization of aptamer strands through π-π stacking and electrostatic interactions. As a result, the Au@HPCN-222 composite demonstrated exceptional efficacy as a substrate for immobilizing the aptamer (Apt) onto the GCE surface. By employing differential pulse voltammetry (DPV) we successfully achieved the detection of chloramphenicol (CAP) with a remarkably low limit of detection of 0.0138 ng mL−1 and the peak DPV currents at 0.18 V (vs. Ag/AgCl) were used for calibration. Furthermore, this aptasensor exhibited high selectivity and reproducibility.

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Acknowledgements

The authors gratefully acknowledge the financial support of this work by the National Natural Science Foundation of China (Grant No. 22008011 and 22108170), Project of Yulin Science and Technology Bureau (Grant No. CXY-2020-023), Shaanxi Province Postdoctoral Science Foundation (No. 2018BSHGZZHQYXMZZ27), National College Student’s Innovation and Entrepreneurship Training Program (No. S202210710261).

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Author notes

  1. Jiang Li, Lingli Qu and Huitong Li contributed equally to this work.

Authors and Affiliations

  1. School of Materials Science and Engineering, Chang’ an University, Xi’an, 710062, Shaanxi, China

    Jiang Li, Huitong Li, Lu Zhao, Tongdan Chen, Jiaying Liu & Yuting Gao

  2. Shanghat University of Medicine Health Sciences Affiliated Zhoupu Hospital, Shanghai, 201300, China

    Lingli Qu & Hongzhi Pan

  3. Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China

    Lingli Qu & Hongzhi Pan

Authors
  1. Jiang Li
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  2. Lingli Qu
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Contributions

Jiang Li: Formal analysis, Writing—review & editing, Writing—original draft. Lingli Qu: Methodology, Formal analysis, Investigation. Huitong Li: Methodology, Investigation. Lu Zhao: Validation, Formal analysis. Tongdan Chen, Jiaying Liu and Yuting Gao: Visualization, Resources. Hongzhi Pan: Review & editing.

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Correspondence to Jiang Li or Hongzhi Pan.

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Li, J., Qu, L., Li, H. et al. An electrochemical aptasensor for the detection of chloramphenicol based on ultra-small Au-inserted hollow PCN-222 MOF. Microchim Acta 190, 366 (2023). https://doi.org/10.1007/s00604-023-05949-y

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