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Disposable syringe-based visual immunotest for pathogenic bacteria based on the catalase mimicking activity of platinum nanoparticle-concanavalin A hybrid nanoflowers

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Abstract

Disposable syringes were used in a novel point-of-care visual test for detecting pathogenic bacteria (Escherichia coli O157:H7 and Salmonella typhimurium). Hybrid nanoflowers composed of platinum nanoparticles and concanavalin A (Pt-nanoflowers) were prepared through a one-pot reaction and were found to be viable catalase mimics. They catalyze the decomposition of hydrogen peroxide (H2O2) to generate O2. When used as labels in immunoassays, they integrate both the functions of biological recognition and signal amplification. The disposable syringe pressure readout was combined with Pt-nanoflower signal conversion and successfully applied to a visual bacteria detection scheme. Both Escherichia coli O157:H7 and Salmonella typhimurium can be quantified with detection limits of as low as 15 and 7 CFU·mL−1, respectively.

One-pot synthetic platinum nanoparticle (PtNP)-concanavalin A hybrid nanoflowers (Pt-nanoflowers), have been used as ideal signal labels for immunoassays and integrating both essential functions of biological recognition and signal amplification. Disposable syringes were used as a readout to detect pathogenic bacteria.

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Acknowledgments

This work was financially supported by the National Key Research and Development Program of China (No. 2016YFD0501001).

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  1. Kui-Yu Wang and Sheng-Jun Bu contributed equally to this paper

Authors and Affiliations

  1. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, 130122, China

    Kui-Yu Wang, Sheng-Jun Bu, Wen-Sen Liu, Zhong-Yi Li & Jia-Yu Wan

  2. The General Hospital of FAW, Changchun, 130011, China

    Chuan-Jing Ju

  3. The Fourth Hospital of Jilin University, Changchun, 130011, China

    Chuan-Jing Ju

  4. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China

    Ye Han

  5. College of Geo-Exploration Science and Technology, Jilin University, Changchun, 130026, China

    Cheng-You Ma

  6. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, China

    Chang-Tian Li

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Wang, KY., Bu, SJ., Ju, CJ. et al. Disposable syringe-based visual immunotest for pathogenic bacteria based on the catalase mimicking activity of platinum nanoparticle-concanavalin A hybrid nanoflowers. Microchim Acta 186, 57 (2019). https://doi.org/10.1007/s00604-018-3133-7

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