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A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass

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Abstract

The present study reports a highly thermostable β-glucosidase (GH3) from Rasamsonia emersonii that was heterologously expressed in Pichia pastoris. Extracellular β-glucosidase was purified to homogeneity using single step affinity chromatography with molecular weight of ~ 110 kDa. Intriguingly, the purified enzyme displayed high tolerance to inhibitors mainly acetic acid, formic acid, ferulic acid, vanillin and 5-hydroxymethyl furfural at concentrations exceeding those present in acid steam pretreated rice straw slurry used for hydrolysis and subsequent fermentation in 2G ethanol plants. Characteristics of purified β-glucosidase revealed the optimal activity at 80 °C, pH 5.0 and displayed high thermostability over broad range of temperature 50–70 °C with maximum half-life of ~ 60 h at 50 °C, pH 5.0. The putative transglycosylation activity of β-glucosidase was appreciably enhanced in the presence of methanol as an acceptor. Using the transglycosylation ability of β-glucosidase, the generated low cost mixed glucose disaccharides resulted in the increased induction of R. emersonii cellulase under submerged fermentation. Scaling up the recombinant protein production at fermenter level using temporal feeding approach resulted in maximal β-glucosidase titres of 134,660 units/L. Furthermore, a developed custom made enzyme cocktail consisting of cellulase from R. emersonii mutant M36 supplemented with recombinant β-glucosidase resulted in significantly enhanced hydrolysis of pretreated rice straw slurry from IOCL industries (India). Our results suggest multi-faceted β-glucosidase from R. emersonii can overcome obstacles mainly high cost associated enzyme production, inhibitors that impair the sugar yields and thermal inactivation of enzyme.

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Funding

Research grant from funding agency Department of Biotechnology with project “Novel concepts for developing efficient cellulolytic cocktail for hydrolysis of bio-refinery relevant pretreated lignocellulosics” [BT/PR31115/PBD/26/766/2019] and the pretreated lignocellulosics materials supplied by IOCL are duly acknowledged.

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Authors and Affiliations

  1. Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Yashika Raheja, Varinder Singh, Gaurav Sharma & Bhupinder Singh Chadha

  2. Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada

    Adrian Tsang

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YR involved in conceptualization, performed experiments, analyzed data, and wrote the manuscript. VS was involved in methodology and data analysis. GS contributed analytical tools and review the manuscript. AT performed data curation and validation. BSC involved in conceptualization, project administration, resources, supervision, Writing-review & editing. All authors read and approved the manuscript.

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Correspondence to Bhupinder Singh Chadha.

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Raheja, Y., Singh, V., Sharma, G. et al. A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass. Bioprocess Biosyst Eng 47, 567–582 (2024). https://doi.org/10.1007/s00449-024-02988-4

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