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Single-cell RNA sequencing to identify cellular heterogeneity and targets in cardiovascular diseases: from bench to bedside

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Abstract

The mechanisms of cardiovascular diseases (CVDs) remain incompletely elucidated. Single-cell RNA sequencing (scRNA-seq) has enabled the profiling of single-cell transcriptomes at unprecedented resolution and throughput, which is critical for deciphering cardiovascular cellular heterogeneity and underlying disease mechanisms, thereby facilitating the development of therapeutic strategies. In this review, we summarize cellular heterogeneity in cardiovascular homeostasis and diseases as well as the discovery of potential disease targets based on scRNA-seq, and yield new insights into the promise of scRNA-seq technology in precision medicine and clinical application.

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Acknowledgements

We thank Shen Song, Liang Chen for providing constructive suggestions. We appreciate Shengkang Huang, Sifei Chen, Xinyi Xiao, Yufei Yang, Yazhuo Wang for discussion, data collection and polishing the manuscript and figures. The figures were created with BioRender.com.

Funding

This work was supported by the National Natural Science Fund for Distinguished Young Scholars of China (82125004) and the National Natural Science Fund for General Program of China (81670376).

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  1. State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China

    Xinjie Xu, Xiumeng Hua, Shengshou Hu & Jiangping Song

  2. Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, 518057, China

    Han Mo

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SH, JS supervised the study. XX wrote the manuscript and prepared the tables and figures. XX, XH, HM, JS and SH participated in the revision of the manuscript. All authors read and approved the final manuscript.

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Xu, X., Hua, X., Mo, H. et al. Single-cell RNA sequencing to identify cellular heterogeneity and targets in cardiovascular diseases: from bench to bedside. Basic Res Cardiol 118, 7 (2023). https://doi.org/10.1007/s00395-022-00972-1

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