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Nitrite-dependent anaerobic methane oxidation bacteria and potential in permafrost region of Daxing’an Mountains

  • Applied Genetics and Molecular Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Nitrite-dependent anaerobic methane oxidation (n-damo) acts as a crucial link between biogeochemical carbon and nitrogen cycles. Nevertheless, very few studies have characterized n-damo microorganisms in high-latitude permafrost regions. Therefore, this study investigated the vertical distribution and diversity of n-damo bacterial communities in soil from three forest types in the permafrost regions of the Daxing'an Mountains. A total of 11 and 8 operational taxonomic units (OTUs) of n-damo 16S rRNA and pmoA genes were observed, respectively. Remarkable spatial variations in n-damo bacteria community richness, diversity, and structure were observed at different soil depths. Moreover, the abundances of n-damo bacteria (16S rRNA and pmoA genes) varied between 1.55 × 104 to 1.47 × 105 and 1.31 × 103 to 3.11 × 104 copies g–1 dry soil (ds), as demonstrated by quantitative PCR analyses. 13CH4 stable isotope tracer assays indicated that the potential n-damo rates varied from 0 to 1.26 nmol g–1 day–1, with the middle layers (20–40 cm and 40–60 cm) exhibiting significantly higher values than the upper (0–20 cm) and deeper layers (80–100 cm) in all three forest types. Redundancy analyses (RDA) indicated that total organic carbon (TOC), nitrate (NO3––N), and nitrite (NO2––N) were key modulators of the distribution of n-damo bacterial communities. This study thus demonstrated the widespread occurrence of n-damo bacteria in cold and high-latitude regions of forest ecosystems and provided important insights into the global distribution of these bacteria.

Key points

• This study detected n-damo bacteria in soil samples obtained from the permafrost region of three forest types in the Daxing'an Mountains.

• The community composition of n-damo bacteria was mainly affected by soil depth and not forest type.

• The abundances of n-damo bacteria first increased and then decreased at higher soil depths.

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Funding

This study was supported by the National Natural Science Foundation of China (42171127) and Harbin Normal University Graduate Innovative Research Project (HSDSSCX2021-20).

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Authors and Affiliations

  1. College of Geographical Sciences, Harbin Normal University, Harbin, 150025, China

    Lu Ren, Xiangwen Wu, Dalong Ma, Lin Liu, Xin Li & Dandan Song

  2. Heilongjiang Province Collaborative Innovation Center of Cold Region Ecological Safety, Harbin, 150025, China

    Lu Ren, Xiangwen Wu, Dalong Ma, Lin Liu, Xin Li & Dandan Song

Authors
  1. Lu Ren
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  2. Xiangwen Wu
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  3. Dalong Ma
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  4. Lin Liu
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  5. Xin Li
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  6. Dandan Song
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Contributions

All the authors contributed to the study conception and design. D. M. conceived and designed the study. L. R., X. W., and X. L. performed the research and analyzed the data. L. R., L. L., and D. S. wrote the manuscript and all the authors revised the manuscript.

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Correspondence to Dalong Ma.

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Ren, L., Wu, X., Ma, D. et al. Nitrite-dependent anaerobic methane oxidation bacteria and potential in permafrost region of Daxing’an Mountains. Appl Microbiol Biotechnol 106, 743–754 (2022). https://doi.org/10.1007/s00253-021-11739-4

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