Abstract
Soil sterilization integrated with agronomic measures is an effective method to reduce soilborne replant diseases. However, the effect of vermicompost or biochar application after soil sterilization on soilborne diseases is poorly understood. A pot experiment was conducted in American ginseng to investigate the effects of vermicompost (VF), biochar (BF), and a combination of vermicompost and biochar (VBF) applied after soil sterilization on the incidence of Fusarium root rot using natural recovery (F) as control. After one growing season, the disease index of root rot, the phenolic acids, and the microbial communities of American ginseng rhizosphere soil were analyzed. The disease index of VF, BF, and VBF decreased by 33.32%, 19.03%, and 80.96%, respectively, compared with F. The highest bacterial richness and diversity were observed in the rhizosphere soil of VBF. Besides, VF and VBF significantly increased the relative abundance of beneficial bacteria (Pseudomonas, Lysobacter, and Chryseolinea) in the rhizosphere soil. Higher concentrations of vanillin, one of the phenolic acids in the roots exudates, were recorded in the rhizosphere soils of BF and VBF. The vanillin concentration showed a significant negative correlation with the disease index. To conclude, vermicompost improved the beneficial bacteria of the rhizosphere soil, while biochar regulated the allelopathic effect of the phenolic acids. The study proposes a combined application of biochar and vermicompost to the rhizosphere soil to control Fusarium root rot of replanted American ginseng effectively.
Key points
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Vermicompost improves the relative abundance of rhizosphere beneficial bacteria.
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Biochar inhibits the degradation of phenolic acids by adsorption.
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The combination of vermicompost and biochar enhances the disease control effect.
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This work was supported by the Natural Science Foundation of Shandong Province of China (ZR2017MH111), the Chinese Academy of Medical Science Innovation Fund for Medical Sciences (2016-I2M-1-012), and the Key Technology Research and Development Program of Shandong Province of China (2018GSF117032).
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WWG and GLT conceived and designed the research. GLT, XLJ, XMZ, and JFL performed the experiments. GLT, YMB, and FBN analyzed data. GLT, YMB, and WWG wrote the manuscript. All authors read and approved the paper.
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Tian, GL., Bi, YM., Jiao, XL. et al. Application of vermicompost and biochar suppresses Fusarium root rot of replanted American ginseng. Appl Microbiol Biotechnol 105, 6977–6991 (2021). https://doi.org/10.1007/s00253-021-11464-y
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DOI: https://doi.org/10.1007/s00253-021-11464-y