Abstract
Purpose
Biogas slurry flooding (BSF) treatment of continuous cropping soil has been demonstrated to effectively inhibit soil-borne pathogens and alleviate root rot in Panax notoginseng. However, it is essential to further explore whether biogas slurry infiltration (BSI) treatment, which creates an anaerobic soil environment similar to that made by BSF that inhibits pathogenic fungi, exerts comparable suppressive effects.
Methods
The effectiveness of BSF and BSI treatments, at two concentrations, in reducing pathogen abundance and alleviating root rot in continuous cropping soil was compared. Changes in soil physicochemical properties were evaluated, and absolute abundances of microbes and root rot ratios were estimated. The fungal community structure was assessed through fungal ITS sequencing, comparing treated and untreated soils.
Results
Both BSF and BSI treatments established an anaerobic/reductive soil environment, resulting in alterations in the fungal community structure. Consequently, pathogen levels were inhibited, leading to reduced soil root rot pathogenicity. In biogas slurry–treated soils, the relative abundance of pathogenic fungi (e.g., Fusarium and Cylindrocarpon) and absolute abundance of fungi, including the pathogenic fungus F. solani, significantly decreased. Moreover, these microbial indices exhibited a significant and positive correlation with the root rot ratio. Notably, potentially inhibitory substances, such as ammonium nitrogen and acetic acid, did not exhibit significant effects on F. solani.
Conclusions
The establishment of an anaerobic environment and changes in the microbial community played a crucial role in inhibiting pathogenic fungi and reducing soil pathogenicity. Both BSF and BSI treatments can be considered appropriate approaches for improving continuous cropping soil.
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Funding
This research was supported by Yunnan Ten Thousand Talents Plan Industrial Technology Leading Talent Project (20191096), Yunnan Basic Research Plan (202001AT070094), Yunnan International Science and Technology Cooperation Project (202003AF140001), Yunnan Basic Research Special Youth Project (202201AU070058), Yunnan Provincial Education Department Scientific Research Fund Project (2022J0127), Yunnan Normal University Doctoral Initiation Project (XJ2021102918), Kunming International (Foreign) Science and Technology Cooperation Base (GHJD-202026), Yunnan Model Worker Innovation Studio, and Yunnan Key Laboratory of Rural Energy Engineering.
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Wang, C., Zhao, X., Li, Q. et al. Alleviation of root rot pathogenicity in the continuous cropping soil of Panax notoginseng by biogas slurry flooding and infiltration treatments. J Soils Sediments 24, 337–348 (2024). https://doi.org/10.1007/s11368-023-03634-4
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DOI: https://doi.org/10.1007/s11368-023-03634-4