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Visual and quantitative detection of E. coli O157:H7 by coupling immunomagnetic separation and quantum dot-based paper strip

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Abstract

Simple and visual quantitative detection of foodborne pathogens can effectively reduce the outbreaks of foodborne diseases. Herein, we developed a simple and sensitive quantum dot (QD)-based paper device for visual and quantitative detection of Escherichia coli (E. coli) O157:H7 based on immunomagnetic separation and nanoparticle dissolution-triggered signal amplification. In this study, E. coli O157:H7 was magnetically separated and labeled with silver nanoparticles (AgNPs), and the AgNP labels can be converted into millions of Ag ions, which subsequently quench the fluorescence of QDs in the paper strip, which along with the readout can be visualized and quantified by the change in length of fluorescent quenched band. Owing to the high capture efficiency and effective signal amplification, as low as 500 cfu mL−1 of E. coli O157:H7 could be easily detected by naked eyes. Furthermore, this novel platform was successfully applied to detect E. coli O157:H7 in spiked milk samples with good accuracy, indicating its potential in the detection of foodborne pathogens in real samples.

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Funding

This work was supported by the Zhejiang Provincial Natural Science Foundation of China (Grant No. LQ20C200008) and Ningbo civil Public Welfare Technology Application Research Project (Grant No. 202002N3064).

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Authors and Affiliations

  1. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, Zhejiang, China

    Zhaohui Qiao, Qiqi Cai & Wenge Yang

  2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Yingchun Fu

  3. State Key Laboratory of Chem/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, Zhejiang, China

    Chunyang Lei

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  1. Zhaohui Qiao
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Correspondence to Chunyang Lei or Wenge Yang.

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Qiao, Z., Cai, Q., Fu, Y. et al. Visual and quantitative detection of E. coli O157:H7 by coupling immunomagnetic separation and quantum dot-based paper strip. Anal Bioanal Chem 413, 4417–4426 (2021). https://doi.org/10.1007/s00216-021-03395-4

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