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Colorimetric immunoassay for rapid detection of Vibrio parahaemolyticus

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Abstract

Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common food-borne pathogens. The authors describe a rapid colorimetric assay for V. parahaemolyticus that is based on a combination of a magnetic bead-based sandwich immunoassay and signal amplification via an enzmye mimic. MnO2 nanoparticles are used as an artificial oxidase that oxidizes 3,3′,5,5′-tetramethylbenzidine in the presence of oxygen to form a blue (and readily visible) product with an absorption maximum at 652 nm. By combining the superior capture efficiency of magnetic beads with the high catalytic activity of the enzmye mimic, this method can detect V. parahaemolyticus concentration in the range between 10 to 105 cfu·mL−1 without pre-enrichment, and the limit of detection is as low as 10 cfu·mL−1. Recoveries ranging from 87.5% to 106.0% are found when analyzing spiked oyster samples. The assay is rapid, sensitive, and specific and specific. In our perception, it shows promise in rapid instrumental and on-site visual detection of V. parahaemolyticus.

Schematic of colorimetric immunoassay for Vibrio parahaemolyticus detection that combines the superior capture efficiency of magnetic beads with the high catalytic activity of an enzyme mimic. The visual detection method shows high sensitivity and specificity.

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Acknowledgements

All the authors acknowledge the Jilin University shared instrumentation facility, and School of Public Health, Jilin University for providing bacteria. This work was supported by the Chinese National Natural Science Foundation (Grant No. 81473018, 81602894, and 81602895) and China Postdoctoral Science Foundation (2017 T100214, 2016 M591492).

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  1. Yushen Liu and Chao Zhao contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Jilin University, Changchun, Jilin, China

    Yushen Liu, Chao Zhao, Xiuling Song, Kun Xu, Juan Wang & Juan Li

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  1. Yushen Liu
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  2. Chao Zhao
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  3. Xiuling Song
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  4. Kun Xu
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  5. Juan Wang
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  6. Juan Li
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Correspondence to Juan Wang or Juan Li.

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Liu, Y., Zhao, C., Song, X. et al. Colorimetric immunoassay for rapid detection of Vibrio parahaemolyticus . Microchim Acta 184, 4785–4792 (2017). https://doi.org/10.1007/s00604-017-2523-6

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  • DOI: https://doi.org/10.1007/s00604-017-2523-6

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