Abstract
Effect of pH on production of extracellular virulence factors ofPseudomonas aeruginosa grown on catheter in biofilm was determined. Alginate and proteinase production was higher at pH 8; in contrast, siderophores (pyochelin and pyoverdin) were synthesized more intensively at pH 5.
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References
Ankenbauer R., Sriyosachati S., Cox C.D.: Effects of siderophores on the growth ofPseudomonas aeruginosa in human serum and transferrin.Infect.Immun.49, 132–140 (1985).
CDC: Nosocomial infection surveillance (1980–1982), p. 155 inCDC Surveillance Summaries 32 (no. 455). Centers for Disease Control, Atlanta (USA) 1983.
Černohorská L., Votava M.: Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria.Folia Microbiol.49, 75–78 (2004).
Cox C.D.: Effect of pvochelin on the virulence ofPseudomonas aeruginosa.Inject.Immun.36, 17–23 (1982).
Delancy-Pulchini E.: Bacterial biofilms: a review of current research.Nephrologie22, 439–451 (2001).
Dworniczek E., Kuzko K., Mróz E., Wojciech Ł., Adamski R., Sobieszczańska B., Seniuk A.: Virulence factors andin vitro adherence ofEnterococcus strains to urinary catheters.Folia Microbiol.48, 671–678 (2003).
Gagnière H., Di Martino P.: α5β1 integrins and fibronectin are involved in adherence of thePseudomonas aeruginosa ER97314 clinical strain to A549 cells.Folia Microbiol.49, 757–762 (2004).
Gargan R.A., Hamilton-Miller J.M.T., Brumfitt W.: Effect of pH and osmolarity onin vivo phagocytosis and killing by neutrophils in urine.Infect.Immun.61, 8–12 (1993).
Heyde M., Coll J.L., Portalier R.: Regulation of major outer membrane porin proteins ofEscherichia coli K 12 by pH.Mol.Gen.Genet208, 511–517 (1987)
Hirakata Y., Furuya N., Tateda K., Matsumoto T., Yamaguchi K.: The influence of exoenzyme S and proteinase on endogenousPseudomonas aeruginosa bacteremia in mice.J.Med.Microbiol.43, 258–261 (1995).
Kadry A.A.: Lack of efflux mechanism in a clinical isolate ofPseudomonas aeruginosa highly resistant to β-lactams and imipenem.Folia Microbiol.48, 529–534 (2003).
Ladd T.I., Schmiek D., Nickel J.C., Costerton J.W.: Rapid method for detection of adherent bacteria on Foley urinary catheters.J.Clin.Microbiol.21, 1004–1006 (1985).
Lamont I.L., Beare P.A., Ochsner U., Vasil A.I., Vasil M.L.: Siderophore-mediated signaling regulates virulence factor production inPseudomonas aeruginosa.Proc.Nat.Acad.Sci.USA99, 7072–7077 (2002).
Majtan V., Hoštacká A., Majtánová L’., Trupl J.: Toxinogenicity and markers of pathogenicity ofPseudomonas aeruginosa strains isolated from patients with tumor diseases.Folia Microbiol.47, 445–449 (2002).
Mathee K., Ciofu O., Sternberg G., Lindum P.W., Campbell J.J., Jensen P., Johnsen A.H., Givskov M., Ohman D.E., Molin S., Hoiby N., Kharazmi A.: Mucoid conversion ofPseudomonas aeruginosa by H2O2: a mechanism for virulence activation in the cystic fibrosis lung.Microbiology145, 1349–1357 (1999).
Morihara K.: Production of elastase and protease byPseudomonas aeruginosa.J.Bacteriol.88, 745–757 (1964).
Ohman D.E., Chakraborty A.M.: Utilization of human respiratory secretion by mucoidPseudomonas aeruginosa of cystic fibrosis origin.Infect.Immun.37, 662–669 (1982).
Pratt L.A., Hsing W., Gibson K.E., Silhavy T.J.: From acids to OsmZ: multiple factors influence synthesis of the OmpF and OmpC porins inE. coli.Mol.Microbiol.20, 911–917 (1996).
Růžička F., Hola V., Votava M., Tejkalova R., Horvát R., Heroldova M., Woznicová V.: Biofilm detection and the clinical significance ofStaphylococcus epidermidis isolates.Folia Microbiol.49, 596–600 (2004).
Smith E.L., Hill R.L., Lehman I.R.: Nature and composition of urine, pp. 199–202 inPrinciples of Biochemistry: Mammalian Biochemistry. McGraw Hill International, Auckland (New Zealand) 1983.
Sriyosachati S., Cox C.D.: Siderophore mediated iron acquisition from transferrin byPseudomonas aeruginosa.Infect.Immun.52, 885–891 (1986).
Terry J.M., Pina S.E., Mattingly S.J.: Environmental conditions which influence mucoid conversion inPseudomonas aeruginosa PAOI.Infect.Immun.59, 471–477 (1991).
Todt J.C., Rocque W.J., Mcgroarty F.J.: Effects of pH on bacterial porin function.Biochemistry31, 10471–10478 (1992).
Visca P., Chiarini F., Mansi A., Vetriani C., Serino L., Orsi N.: Virulence determinants inPseudomonas aeruginosa strains from urinary tract infections.Epidemiol.Infectol.108, 323–336 (1992).
Woods D.E., Vasil M.L.: Pathogenesis ofPseudomonas aeruginosa infections, pp. 21–50 in A.L. Baltch, R.P. Smith (Eds): Pseudomonas aeruginosaInfections and Treatment. Marcel Dekker, New York 1993.
Woods D.E., Lam J.S., Parenchych W., Speet D.P., Campbell M., Godfrey A.J.: Correlation of virulence factors from clinical and environmental isolates with pathogenicity in the neutropenic mouse model.Can.J.Microbiol.43, 541–551 (1997).
Yadav V., Harjai K., Kaur R., Joshi K., Sharma S.: Urovirulence ofPseudomonas aeruginosa: planktonic cellsvs. biofilm cells.Folia Microbiol.49, 465–470 (2004).
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Harjai, K., Khandwaha, R.K., Mittal, R. et al. Effect of pH on production of virulence factors by biofilm cells ofPseudomonas aeruginosa . Folia Microbiol 50, 99–102 (2005). https://doi.org/10.1007/BF02931455
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DOI: https://doi.org/10.1007/BF02931455