Summary
Electrophoretic analysis of total membrane proteins of Escherichia coli cells grown at neutral or acid pH showed that ompF, ompC and lamB porin gene expression was regulated by changes in extracellular pH. Growth at acid pH was correlated with a decrease in outer membrane proteins OmpF and LamB and an increase in protein OmpC. pH-induced effects were confirmed and quantitatively estimated by using strains carrying ompF-lacZ, ompC-lacZ and lamB-lacZ fusions. Our studies showed that the pH-dependent regulation acted at a transcriptional level on ompF and ompC gene expression and also at a post-transcriptional level on ompF gene expression. Similar studies carried out with envZ strains showed that the pH-controlled transducing signal was mediated via the EnvZ protein, although other pH-dependent pathways were also involved.
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References
Bachmann BJ (1983) Linkage map of Escherichia coli K 12. Edition 7. Microbiol Rev 47:180–230
Costerton JW, Ingram JM, Cheng KJ (1974) Structure and function of the cell envelope of gram negative bacteria. Bacteriol Rev 38:87–110
Garrett S, Taylor RK, Silhavy TJ (1983) Isolation and characterization of chain-terminating nonsense mutations in a porin regulator gene, envZ. J Bacteriol 156:62–69
Hall MN, Silhavy TJ (1979) Transcriptional regulation of Escherichia coli K 12 major outer membrane protein 1 b. J Bacteriol 140:342–350
Hall MN, Silhavy TJ (1981a) The ompB locus and the regulation of the outer membrane porin proteins of Escherichia coli K 12. J Mol Biol 146:23–43
Hall MN, Silhavy TJ (1981b) Genetic analysis of the ompB locus in Escherichia coli K 12. J Mol Biol 151:1–15
Heyde M, Portalier R (1983) Isolation and characterization of a new type of Escherichia coli K 12 phoB mutants. Mol Gen Genet 190:122–127
Kawaji H, Mizuno T, Mizushima S (1979) Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins 0–8 and 0–9 of Escherichia coli K 12. J Bacteriol 140:843–847
Lugtenberg B, Peters R, Bernheimer H, Berendsen W (1976) Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Mol Gen Genet 147:251–262
Lundrigan MD, Earhart CF (1984) Gene envY of Escherichia coli K 12 affects thermoregulation of major porin expression. J Bacteriol 157:262–268
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mizuno T, Wurtzel ET, Inouye M (1982) Cloning of the regulatory genes (ompR and envZ) for the matrix proteins of the Escherichia coli outer membrane. J Bacteriol 150:1462–1466
Moreno F, Fowler AV, Hall MN, Silhavy TJ, Zabin I, Schwartz M (1980) A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm. Nature 286:356–359
Ozawa Y, Mizushima S (1983) Regulation of outer membrane porin protein synthesis in Escherichia coli K 12: ompF regulates the expression of ompC. J Bacteriol 154:669–675
Pugsley AP (1985) Escherichia coli K 12 strains for use in the identification and characterization of colicins. J Gen Microbiol 131:369–376
Taylor RK, Hall MN, Silhavy TJ (1983) Isolation and characterization of mutations altering expression of the outer membrane porin proteins using the local anaesthetic procaine. J Mol Biol 166:273–282
Tommassen J, Lugtenberg B (1980) Outer membrane protein of Escherichia coli K 12 is co-regulated with alkaline phosphatase. J Bacteriol 143:151–157
Torriani AM (1967) Alkaline phosphatase from Escherichia coli. In: Cantoni GL, Davies DL (eds) Procedures in nucleic acid research. Harper and Row New York, pp 224–235
Van Alphen W, Lugtenberg B (1977) Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli K 12. J Bacteriol 131:623–630
Verhoef C, Lugtenberg B, Van Boxtel R, DeGraaf P, Verheij H (1979) Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K 12. Mol Gen Genet 169:137–146
Wandersman C, Moreno F, Schwartz M (1980) Pleiotropic mutations rendering Escherichia coli K 12 resistant to bacteriophage TP1. J Bacteriol 143:1374–1383
Wanner BL, Sarthy A, Beckwith J (1979) Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins. J Bacteriol 140:229–239
Worcel A, Burgi E (1974) Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol 82:91–105
Yagil E, Bracha M, Lifshitz Y (1975) The regulatory nature of the phoB gene for alkaline phosphatase synthesis in Escherichia coli. Mol Gen Genet 137:11–16
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Communicated by J. Lengeler
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Heyde, M., Portalier, R. Regulation of major outer membrane porin proteins of Escherichia coli K 12 by pH. Mol Gen Genet 208, 511–517 (1987). https://doi.org/10.1007/BF00328148
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DOI: https://doi.org/10.1007/BF00328148