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Tissue-Culture-Mediated Biotechnological Intervention in Centella asiatica: A Potential Antidiabetic Plant

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Biotechnology of Anti-diabetic Medicinal Plants

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Abstract

Centella asiatica L. (Apiaceae) commonly known as Indian Pennywort is a medicinally important perennial herb widely used in traditional Indian and Chinese systems of medicine for treating various disorders. Bioactive compounds such as asiaticoside possess antidiabetic properties by regulating insulin sensitizer receptors responsible for the treatment of diabetic mellitus. The conventional mode of propagation of C. asiatica using stem cuttings is quite slow and is not sufficient to meet the increasing market demands. Therefore, micropropagation is being used as a reliable and alternative method for propagation. The success of any tissue culture depends on the survival of plantlets after transfer to field conditions. Another important factor that is vital for conservation is the regeneration of plants with uniform genetic makeup. This chapter provides a comprehensive analysis of the plant growth regulator combinations employed for culture initiation, proliferation, and eventual protocol optimization required for successful large-scale in vitro regeneration of C. asiatica. Besides, this chapter offers a detailed analysis of various acclimatization strategies used till date for successful establishment of in vitro regenerated C. asiatica plantlets under in vivo conditions. This chapter also summarizes the employment of different molecular, morphological, biochemical, and cytological markers used for assessing the clonal fidelity among donor plants and tissue-culture-derived plantlets of C. asiatica.

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References

  • Ahmad FB, Ismail G (2003) Medicinal plants used by Kadazandusun communities around Crocker Range. ASEAN Rev Biodivers Environ Conserv (ARBEC) 1:1–10

    Google Scholar 

  • Akin-Idowu PE, Ibitoye DO, Ademoyegun OT (2009) Tissue culture as a plant production technique for horticultural crops. Afr J Biotechnol 8:3782–3788

    Google Scholar 

  • Alagumanian S, Jahirhussain G, Rao MV (2015) High frequency shoot tip multiplication and ex vitro rooting of Centella asiatica (L.) Urb.—a industrial medicinal herb. World J Pharm Res 4:1983–1998

    CAS  Google Scholar 

  • Alqahtani A, Cho JL, Wong KH, Li KM, Razmovski-Naumovski V, Li GQ (2017) Differentiation of three centella species in Australia as inferred from morphological characteristics, ISSR molecular fingerprinting and phytochemical composition. Front Plant Sci 8:1980

    Article  PubMed  PubMed Central  Google Scholar 

  • Arora D, Kumar M, Dubey SD (2002) Centella asiatica—a review of its medicinal uses and pharmacological effects. J Nat Remed 2:143–149

    Google Scholar 

  • Asadi-Samani M, Moradi MT, Mahmoodnia L, Alaei S, Asadi-Samani F, Luther T (2017) Traditional uses of medicinal plants to prevent and treat diabetes; an updated review of ethnobotanical studies in Iran. J Nephropathol 6:118

    Article  PubMed  PubMed Central  Google Scholar 

  • Awang DV (1998) Gotu Kola. Can J Pharmacol 131:42–46

    Google Scholar 

  • Bandara MS, Lee EL, Thomas JE (2011) Gotu Kola (Centella asiatica L.): an under utilized herb. Am J Plant Sci Biotechnol 5:20–31

    Google Scholar 

  • Banerjee S, Zehra M, Kumar S (1999) In vitro multiplication of Centella asiatica, a medicinal herb from leaf explants. Curr Sci 76:147–148

    Google Scholar 

  • Bhandari AK, Baunthiyal M, Bisht VK, Singh N, Negi JS (2013) A quick bud breaking response of a surface model for rapid clonal propagation in Centella asiatica (L.). Int J Biotechnol Mol Biol Res 4(6):93–97

    Article  Google Scholar 

  • Bhavan BV (1992) Selected medicinal plants of India: a monograph of identity, safety and clinical usage. Tata Press, Bombay, India, p 387

    Google Scholar 

  • Bhavna D, Jyoti K (2011) Centella asiatica: the elixir of life. Int J Res Ayur Pharma 2:431–438

    Google Scholar 

  • Bibi Y, Zia M, Nisa S, Habib D, Waheed A, Chaudhary FM (2011) Regeneration of Centella asiatica plants from non-embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plants. J Biol Eng 5:3–8

    Article  Google Scholar 

  • Biradar SR (2017) Somatic embryogenesis of medicinally important herb Centella asiatica L. Biosci Discov 8:295–299

    Google Scholar 

  • Biradar SR, Rachetti BD (2016) Qualitative and quantitative analysis of micropropagated Centella asiatica L. Urb. IOSR J Pharm 6(2):72–76

    CAS  Google Scholar 

  • Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Dańczak-Pazdrowska A, Brzezińska M (2014) Centella asiatica in dermatology: an overview. Phytotherapy Res 28(8):1117–1124

    Article  CAS  Google Scholar 

  • da Rocha Corrêa L, Paim DC, Schwambach J, Fett-Neto AG (2005) Carbohydrates as regulatory factors on the rooting of Eucalyptus saligna Smith and Eucalyptus globulus Labill. Plant Growth Regul 45(1):63–73

    Article  Google Scholar 

  • Dhar ML, Dhar MM, Dhavan BN, Mehrotra BN, Ray C (1968) Screening of Indian medicinal plants for biological activity. Indian J Exp Biol 6:232

    CAS  PubMed  Google Scholar 

  • Fitrianda E, Sukandar EY, Elfahmi E, Adnyana IK (2017) Antidiabetic activity of extract, fractions, and asiaticoside compound isolated from Centella asiatica Linn. Leaves in alloxan-induced diabetic mice. Asian J Pharm Clin Res 10:268–272

    Article  CAS  Google Scholar 

  • Gaddaguti V, Reddy KS, Deepthi RS, Lavanya K, Rama C, Talluri VR, Allu PR (2013) In vitro flower induction and multiple shoot regeneration studies in Centella asiatica from nodal and leaf explants. Ann Plant Sci 2:55–58

    Google Scholar 

  • Gandi S, Giri A (2013) Production and quantification of asiatic acid from in vitro raised shoots and callus cultures of Centella asiatica (L.) Urban. Ann Phytomed 2:95–101

    CAS  Google Scholar 

  • Gantait S, Kundu S, Ali N, Sahu NC (2015) Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix. Acta Physiol Plant 37:98

    Article  Google Scholar 

  • Govarthanan M, Rajinikanth R, Kamala-Kannan S, Selvankumar T (2015) A comparative study on bioactive constituents between wild and in vitro propagated Centella asiatica. J Gen Eng Biotechnol 13:25–29

    Article  Google Scholar 

  • Grundy SM (2006) Drug therapy of the metabolic syndrome: minimizing the emerging crisis in polypharmacy. Nat Rev Drug Discov 5:295–309

    Article  CAS  PubMed  Google Scholar 

  • Gupta AK, Sharma M (2007) Reviews on Indian medicinal plants, vol 5. Indian Council of Medicinal Research 79, pp 965–985

    Google Scholar 

  • Habib MA, Akter S, Banu NA, Rahman RB, Banu TA, Khan S, Islam S (2012) In-vitro regeneration of an important medicinal plant Centella asiatica L. Urban. Bangladesh J Sci Ind Res 47:269–272

    Article  Google Scholar 

  • Hashim P, Sidek H, Helan MHM, Sabery A, Palanisamy UD, Ilham M (2011) Triterpene composition and bioactivities of Centella asiatica. Molecules 16:1310–1322

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hossain SN, Rahman S, Joydhar A, Islam S, Hossain M (2000) In vitro propagation of thankuni (Centella asiatica L.). Plant Tissue Cult 10:17–23

    Google Scholar 

  • Hossain, MA, Hossain MT, Ali MR, Rahman SM (2005) Effect of different carbon sources on in vitro regeneration of Indian pennywort (Centella asiatica L.) (No. Research)

    Google Scholar 

  • Hu X, Sato J, Oshida Y, Xu M, Bajotto G, Sato Y (2003) Effect of Gosha-jinki-gan (Chinese herbal medicine: Niu-Che-Sen-Qi-Wan) on insulin resistance in streptozotocin-induced diabetic rats. Diabetes Res Clin Pract 59:103–111

    Article  PubMed  Google Scholar 

  • Hung TM, Hoang DM, Kim JC, Ahn JS, Min BS (2009) Protein tyrosine phosphatase 1B inhibitory by dammaranes from Vietnamese Giao-Co-Lam tea. J Ethnopharmacol 124:240–245

    Article  CAS  PubMed  Google Scholar 

  • James J, Tugizimana F, Steenkamp P, Dubery I (2013) Metabolomic analysis of methyl jasmonate-induced triterpenoid production in the medicinal herb Centella asiatica (L.) Urban. Molecules 18:4267–4281

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Joshee N, Biswas BK, Yadav AK (2007) Somatic embryogenesis and plant development in Centella asiatica L., a highly prized medicinal plant of the tropics. HortSci 42:633–637

    Article  CAS  Google Scholar 

  • Joshi K, Chaturvedi P, Shubhpriya S (2013) Efficient in vitro regeneration protocol of Centella asiatica (L.) Urban: an endemic and underutilized nutraceutical herb. Afr J Biotechnol 12:5164–5172

    Article  CAS  Google Scholar 

  • Kaensaksiri T, Soontornchainaksaeng P, Soonthornchareonnon N, Prathanturarug S (2011) In vitro clonal propagation of Centella asiatica (L.) Urban using shoot tip explants pre-treated with thidiazuron. Med Plants-Int J Phytomed Relat Ind 3(1):47–52

    Google Scholar 

  • Karthikeyan K, Chandran C, Kulothungan S (2009) Rapid clonal multiplication through in vitro axillary shoots proliferation of Centella asiatica L. Ind J Biotechnol 8:232–235

    CAS  Google Scholar 

  • Kartnig T, Hoffmann-Bohm K (1992) Centella. Hager’s Handbuch der Pharmazeutischen. Praxis 4:764–771

    Google Scholar 

  • Kim OT, Kim MY, Huh SM, Ahn JC, Seong NS, Hwang B (2004a) Effect of growth regulators on asiaticoside production in whole plant cultures of Centella asiatica (L.) Urban. J Plant Biol 47:361–365

    Article  CAS  Google Scholar 

  • Kim OT, Kim MY, Hong MH, Ahn JC, Hwang B (2004b) Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors. Plant Cell Rep 23:339–344

    Article  CAS  PubMed  Google Scholar 

  • Kim OT, Bang KH, Shin YS, Lee MJ, Jung SJ, Hyun DY, Kim YC, Seong NS, Cha SW, Hwang B (2007) Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) Urban elicited by methyl jasmonate. Plant Cell Rep 6:1941–1949

    Article  Google Scholar 

  • Krishnan ML, Roy A, Bharadvaja N (2019) Elicitation effect on the production of asiaticoside and asiatic acid in shoot, callus and cell suspension culture of Centella asiatica. J Appl Pharm Sci 9(06):067–074

    Article  CAS  Google Scholar 

  • Kumar MS (2017) Rapid in vitro multiplication of Centella asiatica (L.) Urban through multiple shoots from leaf explants. Eur J Biotechnol Biosci 5:41–47

    Google Scholar 

  • Kumar N (ed) (2018) Biotechnological approaches for medicinal and aromatic plants: conservation, genetic improvement and utilization. Springer, Singapore

    Google Scholar 

  • Kumari S, Kumar N, Trivedi MP (2018a) In vitro culture and production of secondary metabolites in Centella asiatica. In: Kumar N (ed) Biotechnological approaches for medicinal and aromatic plants. Springer, Singapore, pp 157–173

    Chapter  Google Scholar 

  • Kumari R, Abbas SA, Singh A (2018b) Micropropagation of Centella asiatica through tissue culture an attempt to develop an efficient protocol for large scale production. Indian J Sci Res 8:41–45

    CAS  Google Scholar 

  • Kundu K, Roy A, Saxena G, Kumar L, Bharadvaja N (2016) Effect of different carbon sources and elicitors on shoot multiplication in accessions of Centella asiatica. Med Aromat Plant 5:2167-0412

    Article  Google Scholar 

  • Lai KS, Abdullah P, Yusoff K, Mahmood M (2011) An efficient protocol for particle bombardment-mediated transformation of Centella asiatica callus. Acta Physiol Plant 33(6):2547

    Article  Google Scholar 

  • David LB, L.Ac (1998) Medicine at your feet: healing plants of the Hawaiian kingdom Centella asiatica (Pohe kula)

    Google Scholar 

  • Leung AY, Foster S (1996) Encyclopedia of common natural ingredients used in food drugs and cosmetics, 2nd edn. Wiley, New York, p 848

    Google Scholar 

  • Ling APK, Chin MF, Hussein S (2009) Adventitious root production of Centella asiatica in response to plant growth regulators and sucrose concentration. Med Aromat Plant Sci Biotechnol 3:36–41

    Google Scholar 

  • Loc NH, Giang NT (2012) Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiatica L. Urban). Chem Papers 66(7):642–648

    Article  CAS  Google Scholar 

  • Long HS, Stander MA, Van Wyk BE (2012) Notes on the occurrence and significance of triterpenoids (asiaticoside and related compounds) and caffeoyl quinic acids in Centella species. S Afr J Bot 82:53–59

    Article  CAS  Google Scholar 

  • Mahato RB, Chaudhary RP (2005) Ethnomedicinal study and antibacterial activities of selected plants of Palpa district, Nepal. Ram Chandra Sharma and Madhusudhan Upadhyaya 3:1

    Google Scholar 

  • Mali RG, Hatapakki BC (2008) An in vitro study of effect of Centella asiatica on phagocytosis by human neutrophils. Int J Pharm Sci Nano 1(3):297–302

    Google Scholar 

  • Mangas S, Bonfill M, Osuna L, Moyano E, Tortoriello J, Cusido RM, Piñol MT, Palazón J (2006) The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants. Phytochemistry 67:2041–2049

    Article  CAS  PubMed  Google Scholar 

  • Martin KP (2004) Plant regeneration through somatic embryogenesis in medicinally important Centella asiatica L. In Vitro Cell Dev Biol Plant 40:586–591

    Article  CAS  Google Scholar 

  • Moghaddam SS, Jaafar HB, Aziz MA, Ibrahim R, Rahmat AB, Philip E (2011) Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. Molecules 16:8981–8991

    Article  CAS  PubMed  Google Scholar 

  • Mohapatra H, Barik DP, Rath SP (2008) In vitro regeneration of medicinal plant Centella asiatica. Biol Plantarum 52:339–342

    Article  CAS  Google Scholar 

  • Monaco LC, Lopes CR, Carelli ML (1977) Isomeros de acido chloragenicoem species de coffea. Cinecia Cultura, Recife 26:240

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Naidu TB, Rao SN, Mani NS, Mohan YJ, Pola S (2010) Conservation of an endangered medicinal plant Centella asiatica through plant tissue culture. Drug Invent Today 2:17–21

    Google Scholar 

  • Nath S, Buragohain AK (2003) In vitro method for propagation of Centella asiatica (L) Urban by shoot tip culture. J Plant Biochem Biotechnol 12(2):167–169

    Article  CAS  Google Scholar 

  • Nath S, Buragohain AK (2005) Establishment of callus and cell suspension cultures of Centella asiatica. Biol Plant 49:411–413

    Article  Google Scholar 

  • Nitsch JP, Nitsch C (1956) Studies on the growth of coleoptile and first internode sections. A new, sensitive, straight-growth test for auxins. Plant Physiol 31:94

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ohwi J (1965) Flora of Japan. National Science Museum, Tokyo, Japan. Smithsonian Institution, Washington, DC, p 1067

    Google Scholar 

  • Padmalatha K, Prasad MNV (2008) Genetic diversity in Centella asiatica (L.) Urb., a memory-enhancing neutraceutical herb, using RAPD Markers. Med Aromat Plant Sci Biotechnol 2:90–95

    Google Scholar 

  • Palengara D (2017) Foliar regeneration in Centella asiatica (L.) Urban (Apiaceae) an important threatened medicinal herb. Int J Adv Res 5:970–974

    Article  CAS  Google Scholar 

  • Panathula CS, Mahadev MD, Naidu CV (2014a) Effect of different carbohydrates on in vitro plant regeneration of Centella asiatica (L.)—an important antijaundice medicinal plant. Int J Med Aromat Plants 4:41–47

    Google Scholar 

  • Panathula CS, Mahadev MDN, Naidu CV (2014b) The stimulatory effects of the antimicrobial agents bavistin, cefotaxime and kanamycin on in vitro plant regeneration of Centella asiatica (L.)—an important antijaundice medicinal plant. Am J Plant Sci 5:279–285

    Article  CAS  Google Scholar 

  • Paramageetham CH, Babu GP, Rao JVS (2004) Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India. Plant Cell Tissue Organ Cult 79:19–24

    Article  CAS  Google Scholar 

  • Patra A, Rai B, Rout GR, Das P (1998) Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regul 24:13–16

    Article  CAS  Google Scholar 

  • Paula PC, Oliveira JTA, Sousa DOB, Alves BGT, Carvalho AFU, Franco OL, Vasconcelos IM (2017) Insulin-like plant proteins as potential innovative drugs to treat diabetes—the Moringa oleifera case study. N Biotechnol 39:99–109

    Article  CAS  PubMed  Google Scholar 

  • Peiris KHS, Kays SJ (1996) Asiatic pennywort [Centella asiatica(L.) Urb.]: a little known vegetable crop. Horttechnology 6:13–18

    Article  Google Scholar 

  • Prasad A, Mathur A, Singh M, Gupta MM, Uniyal GC, Lal RK, Mathur AK (2012) Growth and asiaticoside production in multiple shoot cultures of a medicinal herb, Centella asiatica (L.) Urban, under the influence of nutrient manipulations. J Nat Med 66:383–387

    Article  CAS  PubMed  Google Scholar 

  • Prasad A, Mathur A, Kalra A, Gupta MM, Lal RK, Mathur AK (2013) Fungal elicitor-mediated enhancement in growth and asiaticoside content of Centella asiatica L. shoot cultures. Plant Growth Regul 69:265–273

    Article  CAS  Google Scholar 

  • Prasad A, Singh M, Yadav NP, Mathur AK, Mathur A (2014) Molecular, chemical and biological stability of plants derived from artificial seeds of Centella asiatica (L.) Urban—an industrially important medicinal herb. Ind Crops Prod 60:205–211

    Article  CAS  Google Scholar 

  • Prasad A, Prakash O, Mehrotra S, Khan F, Mathur AK, Mathur A (2017) Artificial neural network-based model for the prediction of optimal growth and culture conditions for maximum biomass accumulation in multiple shoot cultures of Centella asiatica. Protoplasma 254:335–341

    Article  CAS  PubMed  Google Scholar 

  • Rahman MM, Sayeed MS, Haque MA, Hassan MM, Islam SM (2012) Phytochemical screening, antioxidant, anti-Alzheimer and anti-diabetic activities of Centella asiatica. J Nat Prod Plant Resour 2(4):504–511

    Google Scholar 

  • Rahmatullah M, Ferdausi D, Mollik A, Jahan R, Chowdhury MH, Haque WM (2010) A survey of medicinal plants used by Kavirajes of Chalna area, Khulna district, Bangladesh. Afr J Tradit Complement Altern Med 7:91–97

    Article  Google Scholar 

  • Ramírez-Espinosa JJ, Rios MY, López-Martínez S, López-Vallejo F, Medina-Franco JL, Paoli P, Camici G, Navarrete-Vázquez G, Ortiz-Andrade R, Estrada-Soto S (2011) Antidiabetic activity of some pentacyclic acid triterpenoids, role of PTP–1B: in vitro, in silico, and in vivo approaches. Eur J Med Chem 46:2243–2251

    Article  PubMed  Google Scholar 

  • Rodier-Goud M, Rivallan R, Lussert A, Danthu P, de Lamotte F, Ralambofetra E, Ramavovololona P, Noyer JL, Baurens FC (2013) Insight into the biology, genetics and evolution of the Centella asiatica polyploid complex in Madagascar. Ind Crops Prod 47:118–125

    Article  Google Scholar 

  • Roy A, Bharadvaja N (2017) Effect of different culture medias on shoot multiplication and stigmasterol content in accessions of Centella asiatica. Int J Ayurvedic Herbal Med 7:2643–2650

    Google Scholar 

  • Satheesan J, Narayanan AK, Sakunthala M (2012) Induction of root colonization by Piriformospora indica leads to enhanced asiaticoside production in Centella asiatica. Mycorrhiza 22:195–202

    Article  CAS  PubMed  Google Scholar 

  • Seevaratnam V, Banumathi P, Premalatha MR, Sundaram SP, Arumugam T (2012) Functional properties of Centella asiatica (L.): a review. Int J Pharm Pharm Sci 4:8–14

    Google Scholar 

  • Shashikala CM, Shashidhara S, Rajshekharan PE (2009) In vitro callus culture of Centella asiatica L.(Urb.) and its HPLC analysis. Biosci Biotechnol Res Asia 6:863–868

    CAS  Google Scholar 

  • Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhawan BN (1999) In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J Ethnopharmacol 65:1–11

    Article  CAS  PubMed  Google Scholar 

  • Singh S, Gautam A, Sharma A, Batra A (2010) Centella asiatica (L.): a plant with immense medicinal potential but threatened. Int J Pharma Sci Rev Res 4:9–17

    CAS  Google Scholar 

  • Singh G, Kaur B, Sharma N, Bano A, Kumar S, Dhaliwal HS, Sharma V (2014) In vitro micropropagation and cytomorphological evaluation of Centella asiatica (L.) Urban (Mandukparni) from Himachal Pradesh, India-an endemic, endangered and threatened herb. Plant Tissue Cult Biotechnol 24:155–171

    Article  Google Scholar 

  • Sivakumar G, Alagumanian S, Rao MV (2006) High frequency in vitro multiplication of Centella asiatica: an important industrial medicinal herb. Eng Life Sci 6:597–601

    Article  CAS  Google Scholar 

  • Skrzypczak-Pietraszek E, Urbańska A, Żmudzki P, Pietraszek J (2019) Elicitation with methyl jasmonate combined with cultivation in the Plantform™ temporary immersion bioreactor highly increases the accumulation of selected centellosides and phenolics in Centella asiatica (L.) Urban shoot culture. Eng Life Sci 19:931–943

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Solet JM, Simon-Ramiasa A, Cosson L, Guignard JL (1998) VI Centella asiatica (L.) Urban. (Pennywort): cell culture, production of terpenoids, and biotransformation capacity. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, medicinal and aromatic plants X, vol 41. Springer, Berlin

    Google Scholar 

  • Subban R, Veerakumar A, Manimaran R, Hashim KM, Balachandran I (2008) Two new flavonoids from Centella asiatica (Linn.). J Nat Med 62(3):369–373

    Article  CAS  PubMed  Google Scholar 

  • Sudhakaran MV (2017) Botanical pharmacognosy of Centella asiatica (Linn.) Urban. Pharmacogn J 9:546–558

    Article  Google Scholar 

  • Swargiary A, Hankhray B, Brahma BK, Rahman S (2013) Ethno-botanical study of anti-diabetic medicinal plants used by the local people of Kokrajhar district of Bodoland Territorial Council India. J Med Plants Stud 1:51–58

    Google Scholar 

  • Thong-On W, Arimatsu P, Pitiporn S, Soonthornchareonnon N, Prathanturarug S (2014) Field evaluation of in vitro-induced tetraploid and diploid Centella asiatica (L.) Urban. J Nat Med 68:267–273

    Article  CAS  PubMed  Google Scholar 

  • Tiwari KN, Sharma NC, Tiwari V, Singh BD (2000) Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tissue Organ Cult 63:179–185

    Article  CAS  Google Scholar 

  • Tiwari C, Bakshi M, Vichitra A (2013) A rapid two step protocol of in vitro propagation of an important medicinal herb Centella asiatica Linn. Afr J Biotechnol 12:1084–1080

    CAS  Google Scholar 

  • Wang Y, Wang Y, Li K, Song X, Chen J (2016) Characterization and comparative expression profiling of browning response in Medinilla formosana after cutting. Front Plant Sci 7:1–16

    Article  PubMed  PubMed Central  Google Scholar 

  • Wankahar B, Tripathi RS (1990) Competitive fitness of Centella asiatica populations cut from stem cuttings and seedlings. Proc Ind Acad Sci Plant Sci 100:239–245

    Article  Google Scholar 

  • Watkins PJ (2003) ABC of diabetes, cardiovascular disease, hypertension and lipids. Brit Med J (Middle East) 10:82–84

    Google Scholar 

  • Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and Eastern Africa being an account of their medicinal and other uses, chemical composition, pharmacological effects and toxicology in man and animal, 2nd edn. E & S Livingstone, Edinburgh, p 1457

    Google Scholar 

  • Wild S, Roglic G, Green A, Sicree R, King H (2004) Global prevalence of diabetes mellitus, estimates for the year 2000 and projections for 2030. Diabetes Care 27:1047–1053

    Article  Google Scholar 

  • Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40:2837–2849

    Article  CAS  PubMed  Google Scholar 

  • Yoo NH, Kim OT, Kim JB, Kim SH, Kim YC, Bang KH, Hyun DY, Cha SW, Kim MY, Hwang B (2011) Enhancement of centelloside production from cultured plants of Centella asiatica by combination of thidiazuron and methyl jasmonate. Plant Biotechnol Rep 5:283–287

    Article  Google Scholar 

  • Yoosook C, Bunyapraphatsara N, Boonyakiat Y, Kantasuk C (2000) Anti-herpes simplex virus activities of crude water extracts of Thai medicinal plants. Phytomedicine 6:411–419

    Article  CAS  PubMed  Google Scholar 

  • Zhang XG, Han T, He ZG, Zhang QY, Zhang L, Rahman K, Qin LP (2012) Genetic diversity of Centella asiatica in China analyzed by inter-simple sequence repeat (ISSR) markers: combination analysis with chemical diversity. J Nat Med 66(1):241–247

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Centre for Biotechnology, Siksha‘O’Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India

    Priyanka Mohapatra, Asit Ray, I. Sriram Sandeep & Sanghamitra Nayak

  2. Ramadevi Women’s University, Bhubaneswar, Odisha, India

    Sujata Mohanty

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  1. Priyanka Mohapatra
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  2. Asit Ray
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  3. I. Sriram Sandeep
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  4. Sanghamitra Nayak
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  1. Crop Research Unit (Genetics and Plant Breeding), Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal, India

    Saikat Gantait

  2. Institute of Biological Science, SAGE University, Indore, Madhya Pradesh, India

    Sandeep Kumar Verma

  3. Plantation Spices Medicinal & Aromatic Crops, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal, India

    Amit Baran Sharangi

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Mohapatra, P., Ray, A., Sandeep, I.S., Nayak, S., Mohanty, S. (2021). Tissue-Culture-Mediated Biotechnological Intervention in Centella asiatica: A Potential Antidiabetic Plant. In: Gantait, S., Verma, S.K., Sharangi, A.B. (eds) Biotechnology of Anti-diabetic Medicinal Plants. Springer, Singapore. https://doi.org/10.1007/978-981-16-3529-8_4

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