Abstract
The interest in producing pharmaceutical proteins in a nontoxic plant host has led to the development of an approach to express such proteins in transplastomic lettuce (Lactuca sativa L.). A number of therapeutic proteins and vaccine antigen candidates have been stably integrated into the lettuce plastid genome using biolistic DNA delivery. High levels of accumulation and retention of biological activity suggest that lettuce may provide an ideal platform for the production of biopharmaceuticals.
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Acknowledgments
Support was provided by the National Science Foundation (IOS-1027259) and the Fred G. Gloeckner Foundation.
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Ruhlman, T.A. (2014). Plastid Transformation in Lettuce (Lactuca sativa L.) by Biolistic DNA Delivery. In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 1132. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-995-6_21
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DOI: https://doi.org/10.1007/978-1-62703-995-6_21
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Publisher Name: Humana Press, Totowa, NJ
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