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Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS

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Clinical Applications of Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 603))

Abstract

We describe a method for the quantitative analysis of nicotine, cotinine, trans-3′-hydroxy cotinine, nornicotine, and anabasine in urine, serum, and plasma using liquid chromatography-tandem mass spectrometry. A mix of deuterium-labeled internal standards (IS) is added to a specimen aliquot. The aliquot is extracted using mixed-mode solid phase extraction and eluted into an autosampler vial for injection into an LC-MS-MS system. An Atlantis silica column is used for LC separation in hydrophilic interaction mode. Tandem mass spectrometry detection is performed in positive ion mode with electrospray ionization and two multiple reaction monitoring (MRM) transitions monitored for each analyte and IS.

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Authors and Affiliations

  1. ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA

    Bingfang Yue, Mark M. Kushnir, Francis M. Urry & Alan L. Rockwood

  2. Department of Pathology, University of Utah, Salt Lake City, UT, USA

    Francis M. Urry & Alan L. Rockwood

Authors
  1. Bingfang Yue
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  2. Mark M. Kushnir
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  3. Francis M. Urry
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  4. Alan L. Rockwood
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Editor information

Editors and Affiliations

  1. Dept. Pathology & Laboratory Medicine, Children's Mercy Hospital, Gillham Road 2401, Kansas City, 64108, U.S.A.

    Uttam Garg

  2. Dept. Pathology & Laboratory Medicine, University of North Carolina, Chapel Hill, 27599-7525, U.S.A.

    Catherine A. Hammett-Stabler

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Yue, B., Kushnir, M.M., Urry, F.M., Rockwood, A.L. (2010). Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS. In: Garg, U., Hammett-Stabler, C. (eds) Clinical Applications of Mass Spectrometry. Methods in Molecular Biology, vol 603. Humana Press. https://doi.org/10.1007/978-1-60761-459-3_38

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  • DOI: https://doi.org/10.1007/978-1-60761-459-3_38

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-458-6

  • Online ISBN: 978-1-60761-459-3

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