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Simultaneous, rapid, and sensitive quantification of 8-hydroxy-2′-deoxyguanosine and cotinine in human urine by on-line solid-phase extraction LC-MS/MS: correlation with tobacco exposure biomarkers NNAL

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Abstract

Cigarette smoke can increase oxidative DNA damage. The main component in cigarette smoke is nicotine. Nicotine is metabolized to cotinine, which can be regarded as a biomarker for measuring exposure to tobacco smoke. A sensitive, simple, and robust method based on on-line solid-phase extraction liquid chromatography with tandem mass spectrometry (on-line SPE LC-MS/MS) has been developed and validated for the simultaneous determination of 8-OHdG and cotinine. The matrix effects of 8-OHdG and cotinine were measured at 97.1 and 91.7 %, with values for CV at 4.4 and 4.2 %, respectively. The limits of detection of 8-OHdG and cotinine were 10.0 and 5.5 pg mL−1, and the limits of quantification were 40.0 and 20.0 pg mL−1, respectively. The total run time was 12 min. We quantified 8-OHdG and cotinine in the urine of 80 male subjects. The results showed the levels of 8-OHdG and cotinine in smokers were significantly higher than that in non-smokers. Furthermore, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronide conjugate (defined as total NNAL) are the nitrosation metabolites of nicotine. In this study, urinary levels of 8-OHdG and cotinine were well correlated with urinary levels of total NNAL. This is also the first study to focus on the future risk of oxidative stress from exposure to cigarette smoke based on the relationship between 8-OHdG levels, cotinine levels, and total NNAL concentrations in the urine of humans.

On-line SPE LC-MS/MS for the simultaneous determination of 8-OHdG and cotinine in human urine

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Acknowledgments

This study was supported by grants number MOST104-2113-M-030-004 from the Ministry of Science and Technology, Taiwan.

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  1. Department of Chemistry, Fu Jen Catholic University, No. 510 Zhongzheng Road, Xinzhuang District, New Taipei City, 24205, Taiwan

    Chao-Yu Chen, Yu-Ting Jhou & Hui-Ling Lee

  2. Department of Public Health, Fu Jen Catholic University, No. 510 Zhongzheng Road, Xinzhuang District, New Taipei City, 24205, Taiwan

    Yu-Wen Lin

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Chen, CY., Jhou, YT., Lee, HL. et al. Simultaneous, rapid, and sensitive quantification of 8-hydroxy-2′-deoxyguanosine and cotinine in human urine by on-line solid-phase extraction LC-MS/MS: correlation with tobacco exposure biomarkers NNAL. Anal Bioanal Chem 408, 6295–6306 (2016). https://doi.org/10.1007/s00216-016-9741-3

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