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Genetic Diversity in Indian Mustard [Brassica juncea (L.) Czernj & Cosson] as Revealed by Agronomic Traits and RAPD Markers

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Abstract

In present study, an attempt has been made to assess the genetic diversity among 50 genotypes of Indian mustard using agronomic traits and random amplified polymorphic DNA (RAPD) markers. Variability was observed for agronomic traits viz plant height (cm), main shoot length (cm), days to flower initiation, number of siliquae on main raceme, number of seeds per siliqua, seed yield per plot (g), biological yield per plot (g), harvest index (%), days to maturity and 1,000-seed weight (g). Cluster analysis based on agronomic traits revealed a high degree of diversity among the accessions. Maximum variability was recorded for biological yield followed by seed yield, while minimum variability was observed for days to maturity followed by days to flower initiation. RAPD marker OPB8 was found to be the most informative primer followed by OPB14, OPB11 and OPB2. Both agronomic traits and molecular markers were effective in discriminating genotypes into different clusters, however, clustering pattern varied for both agronomic traits and molecular markers. Different clustering pattern based on agronomic data and RAPD markers indicated lack of association between genetic and phenotypic diversity implying that polymorphism due to RAPD is not linked to phenotypic variation. There was no relationship between place of origin and clustering of genotypes, however, diverse genotypes could be identified on the basis of both and are likely to produce more variability in segregating generations as well as to serve as parents for high heterosis.

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References

  1. USDA (2012) United States department of agriculture-rapeseed area, yield and production table no. 15. http://www.fas.usda.gov/psdonline/psdreport.aspx. Accessed 12 June 2012

  2. Ministry of Agriculture (2011) Statistics at a glance. Directorate of Economics and Statistics, Department of Agriculture and Cooperation, Ministry of Agriculture, Government of India, New Delhi

  3. Singh KH, Kumar A, Kumar PR, Chauhan JS, Misra AK, Singh VV, Meena SS, Srivastava KK, Meena ML (2009) Notification of crop varieties and registration of germplasm-Indian mustard hybrid NRCHB 506. Indian J Genet 69:165

    Google Scholar 

  4. Xiao J, Li J, Yuan L, McCouch SR, Tanksley SD (1996) Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers. Theor Appl Genet 92:637–643

    Article  Google Scholar 

  5. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18:6531–6535

    Article  Google Scholar 

  6. Rafalski JA, Tingey SV (1993) Genetic diagnosis in plant breeding: RAPDs, microsatellites and machines. Trends Genet 9:275–280

    Article  Google Scholar 

  7. Betal S, Chowdhury PR, Kundu S, Raychaudhary SS (2004) Estimation of genetic variability of Vigna radiata cultivars by RAPD analysis. Bio Plant 48:205–209

    Article  Google Scholar 

  8. Cordeiro AI, Sanchez-Sevilla JF, Alvarez-Tinaut MC, Gomez-Jimenez MC (2008) Genetic diversity assessment in Portugal accessions of Olea europea by RAPD markers. Bio Plant 52:642–647

    Article  Google Scholar 

  9. Pandey S, Kumar S, Mishra U, Rai A, Singh M, Rai M (2008) Genetic diversity in Indian ash gourd (Benincasa hispida) accessions as revealed by quantitative traits and RAPD markers. Sci Hortic 118:80–86

    Article  Google Scholar 

  10. Choudhury PR, Singh IP, George B, Verma AK, Singh NP (2008) Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis. Bio Plant 52:648–653

    Article  Google Scholar 

  11. Alam MA, Gulati P, Gulati AK, Mishra GP, Naik PK (2009) Assessment of genetic diversity among Podophyllum hexandrum genotypes of the north-western Himalayan region for podophyllotoxin production. Indian J Biotechnol 8:391–399

    Google Scholar 

  12. Jordan-Pla A, Estrelles E, Boscaiu M, Soriano P, Vicente O, Mateu-Andres I (2009) Genetic variability in the endemic Leucojum valentinum. Bio Plant 53:317–320

    Article  Google Scholar 

  13. Padmesh P, Reji JV, Paul B, Mukunthakumar S, Praveen G, Seeni S (2009) Analysis of genetic variability in two diploid Musa cultivars using RAPD. Bio Plant 53:711–714

    Article  Google Scholar 

  14. Ikbal BKS, Dhillon RS (2010) Evaluation of genetic diversity in Jatropha curcas L. using RAPD markers. Indian J Biotechnol 9:50–57

    Google Scholar 

  15. Viana AJC, Souza MM, Araujo IS, Correa RX, Ahnert D (2010) Genetic diversity in Passiflora species determined by morphological and molecular characteristics. Bio Plant 54(3):535–538

    Article  Google Scholar 

  16. Rai N, Singh PK, Rai AC, Rai VP, Singh M (2011) Genetic diversity in Indian bean (Lablab purpureus) germplasm based on morphological traits and RAPD markers. Ind J Agric Sci 81(9):801–806

    Google Scholar 

  17. Batth GS, Brar PS, Chhuneja P, Cheema GS, Kumar H, Vikal Y (2011) Genetic diversity analysis of Indian garlic germplasm using RAPD markers and agro-morphological traits. Crop Improv 389(2):171–178

    Google Scholar 

  18. Kumar A, Shirkot P, Thakur AK, Raina R (2012) Assessment of genetic diversity in Valeriana jatamansi Jones. germplasm using RAPD markers. Natl Acad Sci Lett 35(3):221–226

    Article  Google Scholar 

  19. Jain A, Bhatia S, Banga SS, Prakash S, Lakshmikumaran M (1994) Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor Appl Genet 88:116–122

    Article  Google Scholar 

  20. Fu J, Ming-Fang Z, Xiao-Hua Q (2006) Genetic diversity of traditional Chinese mustard crops Brassica juncea as revealed by phenotypic differences and RAPD markers. Genet Resour Crop Evol 53:1513–1519

    Article  Google Scholar 

  21. Prakash S, Kirti PB, Bhat SR (1998) A Moricandia arvensis based cytoplasmic male sterility and fertility restoration system in B. juncea. Theor Appl Genet 97:488–492

    Article  Google Scholar 

  22. Perrier X, Jacquemoud-Collet JP (2006) DARwin software. http://darwin.cirad.fr/darwin

  23. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  24. Nei M (1987) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    Google Scholar 

  25. Teklewold A, Becker HC (2006) Geographic pattern of genetic diversity among 43 Ethiopian mustard (Brassica carinata A. Braun) accessions as revealed by RAPD analysis. Genet Resour Crop Evol 53:1173–1185

    Article  Google Scholar 

  26. Anand IJ, Rawat DS (1984) Genetic diversity, combining ability and heterosis in brown mustard. Ind J Genet Plant Breed 44:226–234

    Google Scholar 

  27. Gupta VP, Sekhon MS, Satija DR (1991) Studies on genetic diversity, heterosis and combining ability in Indian mustard [Brassica juncea L. (Czern & Coss)]. Ind J Genet Plant Breed 51:448–453

    Google Scholar 

  28. Pooler MR, Simon PW (1993) Characterization and classification of isozyme and morphological variation in a diverse collection of garlic clones. Euphytica 68:121–130

    Article  Google Scholar 

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Acknowledgments

The authors are thankful to the breeders of different institutions who provided seeds of genotypes used in this study.

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Authors and Affiliations

  1. Directorate of Rapeseed-Mustard Research, Bharatpur, 321 303, Rajasthan, India

    K. H. Singh, R. Shakya, A. K. Thakur, D. K. Chauhan & J. S. Chauhan

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  1. K. H. Singh
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  2. R. Shakya
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  3. A. K. Thakur
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  4. D. K. Chauhan
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  5. J. S. Chauhan
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Correspondence to K. H. Singh.

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Singh, K.H., Shakya, R., Thakur, A.K. et al. Genetic Diversity in Indian Mustard [Brassica juncea (L.) Czernj & Cosson] as Revealed by Agronomic Traits and RAPD Markers. Natl. Acad. Sci. Lett. 36, 419–427 (2013). https://doi.org/10.1007/s40009-013-0149-8

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