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Non-aerated liquid culture promotes shoot organogenesis in Eucalyptus globulus Labill

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Abstract

Eucalyptus is very recalcitrant to in vitro culture. In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium to improve shoot proliferation. Cultures were initiated with hypocotyls and leaf segments from plantlets cultivated on semisolid ½ MS modified medium supplemented with 4.44 µM 6-Benzyladenine (BA) and 16.1 µM 1-Naphthaleneacetic acid (NAA). Calli were transferred to shoot induction medium, with either 0.5 or 2.7 µM NAA. Shoot multiplication was carried out on 4.44 µM BA + 0.5 µM NAA medium, and semisolid and non-aerated liquid systems were compared for improving shoot proliferation. Rooting of adventitious shoots was evaluated on medium containing NAA or Indole-3-butyric acid -IBA (5 and 16 µM). Callogenesis was obtained from both types of explants, although shoot formation was only obtained from leaf-derived calli. Shoot proliferation on 4.44 µM BA + 0.5 µM NAA resulted in the most shoots/callus. Non-aerated liquid medium was more efficient in promoting shoot multiplication (53.5 shoots/callus) than was semisolid medium (28.5 shoots/callus). Levels of phenolic compounds were significantly reduced in the shoots cultivated in liquid medium. Efficient rooting (76%) was obtained using 16 µM IBA.

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Acknowledgements

This work was supported by the National Council for Scientific and Technological Development (CNPq)/Brazil, under Grant 477538/2013-4. The authors are grateful to Suzano Papel e Celulose (former RioCell, Brazil) for providing seeds of E. globulus and to Janaína Belquis da S. P. Langois for technical assistance.

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Authors and Affiliations

  1. Laboratory of Plant Biotechnology, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga 6681, 12A, Porto Alegre, RS, 90619-900, Brazil

    T. D. Salla, C. dos S. Silva, K. L. de G. Machado, L. V. Astarita & E. R. Santarém

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  1. T. D. Salla
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  2. C. dos S. Silva
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  3. K. L. de G. Machado
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  4. L. V. Astarita
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  5. E. R. Santarém
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Correspondence to E. R. Santarém.

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The authors declare that they have no conflict of interest.

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Project funding: This work was supported by the National Council for Scientific and Technological Development (CNPq)/Brazil, under Grant 477538/2013-4.

The online version is available at http://www.springerlink.com

Corresponding editor: Chai Ruihai.

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Salla, T.D., Silva, C.S., Machado, K.L.G. et al. Non-aerated liquid culture promotes shoot organogenesis in Eucalyptus globulus Labill. J. For. Res. 29, 623–629 (2018). https://doi.org/10.1007/s11676-017-0485-5

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  • DOI: https://doi.org/10.1007/s11676-017-0485-5

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