Abstract
Linkage maps of the apricot accessions ‘Lito’ and ‘BO 81604311’ were constructed using a total of 185 simple sequence repeat (SSR) markers sampled from those isolated in peach, almond, apricot and cherry; 74 were derived from a new apricot genomic library enriched for AG/CT microsatellite repeats (UDAp series), and in total, 98 had never been mapped in Prunus before. Eight linkage groups putatively corresponding to the eight haploid apricot chromosomes were identified for each parent. The two maps were 504 and 620 cM long, respectively, with 96 anchor markers showing a complete co-linearity between the two genomes. As few as three gaps larger than 15 cM were present in ‘Lito’ and six in the male parent; the maps align well with all the available SSR-based Prunus maps through the many common anchor loci. Only occasionally inverted positions between adjacent markers were found, and this can be explained by the small size of cross populations analysed in these Prunus maps and in those reported in literature. The newly developed apricot SSRs will help saturating the existing Prunus maps and will extend the choice of markers in the development of genetic maps for new breeding populations.
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References
Aranzana MJ, Carbo J, Arus P (2002) Microsatellite variability in peach [Prunus persica (L.) Batsch]: cultivar identification, marker mutation, pedigree inferences and population structure. Theor Appl Genet 106:1341–1352
Aranzana MJ, Pineda A, Cosson P, Dirlewanger E, Ascasibar J, Cipriani G, Ryder CD, Testolin R, Abbott A, King GJ, Iezzoni AF, Arús P (2003) A set of simple-sequence repeat (SSR) markers covering the Prunus genome. Theor Appl Genet 106:819–825
Ballester J, Socias i Company R, Arus P, de Vicente MC (2001) Genetic mapping of a major gene delaying blooming time in almond. Plant Breed 120:268–270
Bliss FA, Arulsekar S, Foolad MR, Becerra V, Gillen AM, Warburton ML, Dandekar AM, Kocsisne GM, Mydin KK (2002) An expanded genetic linkage map of Prunus based on an interspecific cross between almond and peach. Genome 45:520–529
Cipriani G, Lot G, Huang WG, Marrazzo M-T, Peterlunger E, Testolin R (1999) AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L) Batsch]: isolation, characterisation and cross-species amplification in Prunus. Theor Appl Genet 99:65–72
Clarke JB, Tobutt KR (2003) Development and characterization of polymorphic microsatellites from Prunus avium ‘Napoleon’. Mol Ecol Notes 3:578–580
Decroocq V, Fave MG, Hagen L, Bordenave L, Decroocq S (2003) Development and transferability of apricot and grape EST microsatellite markers across taxa. Theor Appl Genet 106:912–922
Decroocq V, Foulongne M, Lambert P, Le Gall O, Mantin C, Pascal T, Schurdi-Levraud V, Kervella J (2005) Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana. Mol Genet Genomics 272:680–689
Dettori MT, Quarta R, Verde I (2001) A peach linkage map integrating RFLPs, SSRs, RAPDs and morphological markers. Genome 44:783–790
Dicenta F, Martinez-Gomez P, Burgos L, Egea J (2000) Inheritance of resistance to plum pox potyvirus (PPV) in apricot, Prunus armeniaca. Plant Breed 119:161–164
Dirlewanger E, Pronier V, Parvery C, Rothan C, Guye A, Monnet R (1998) Genetic linkage map of peach [Prunus persica (L.) Batsch] using morphological and molecular markers. Theor Appl Genet 97:888–895
Dirlewanger E, Moing A, Rothan C, Svanella L, Pronier V, Guye A, Plomion C, Monet R (1999) Mapping QTLs controlling fruit quality in peach (Prunus persica (L.) Batsch). Theor Appl Genet 98:18–31
Dirlewanger E, Cosson P, Tavaud M, Aranzana MJ, Poizat C, Zanetto A, Arús P, Laigret F (2002) Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theor Appl Genet 105:127–138
Dirlewanger E, Graziano E, Joobeur T, Garriga-Caldere F, Cosson P, Howad W, Arus P (2004a) Comparative mapping and marker-assisted selection in Rosaceae fruit crops. Proc Natl Acad Sci USA 101:9891–9896
Dirlewanger E, Cosson P, Howad W, Capdeville G, Bosselu N (2004b) Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid–location of root-knot nematode resistance genes. Theor Appl Genet 109:827–832
Etienne C, Rothan C, Moing A, Plomion C, Bodenes C, Svanella-Dumas L, Cosson P, Pronier V, Monet R, Dirlewanger E (2002) Candidate genes and QTLs for sugar and organic acid content in peach [Prunus persica (L.) Batsch]. Theor Appl Genet 105:145–159
Foulongne M, Pascal T, Arús P, Kervella J (2003) The potential of Prunus davidiana for introgression into peach [Prunus persica (L.) Batsch] assessed by comparative mapping. Theor Appl Genet 107:227–238
Geuna F, Toschi M, Bassi D (2003) The use of AFLP markers for cultivar identification in apricot. Plant Breed 122:526–531
Hagen LS, Chaib J, Fady B, Decroocq V, Bouchet JP, Lambert P, Audergon JM (2004) Genomic and cDNA microsatellites from apricot (Prunus armeniaca L.). Mol Ecol Notes 4:742–745
Howad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG, Arús P (2005) Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map. Genetics 171:1305–1309
Hurtado MA, Romero C, Vilanova S, Abbott AG, Llacer G, Badenes ML (2002) Genetic linkage map of two apricot cultivars (Prunus armeniaca L.) and mapping of PPV (sharka) resistance. Theor Appl Genet 105:182–191
Jáuregui B, de Vicente MC, Messeguer R, Felipe A, Bonnet A, Salesses G, Arús P (2001) A reciprocal translocation between ‘Garfi’ almond and ‘Nemared’ peach. Theor Appl Genet 102:1169–1176
Joobeur T, Viruel MA, de Vicente MC, Jauregui B, Ballester J, Dettori MT, Verde I, Truco MJ, Messeguer R, Battle I, Quarta R, Dirlewanger E, Arús P (1998) Construction of a saturated linkage map for Prunus using an almond × peach F2 progeny. Theor Appl Genet 97:1034–1041
Joobeur T, Periam N, de Vicente MC, King GJ, Arus P (2000) Development of a second generation linkage map for almond using RAPD and SSR markers. Genome 43:649–655
Lambert P, Hagen LS, Arus P, Audergon JM (2004) Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) compared with the almond Texas × peach Earlygold reference map for Prunus. Theor Appl Genet 108:1120–1130
Lopes MS, Sefc KM, Laimer M, Da Câmara Machado A (2002) Identification of microsatellite loci in apricot. Mol Ecol Notes 2:24–26
Lu ZX, Sosinski B, Reighard GL, Baird WV, Abbott AG (1998) Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks. Genome 41:199–207
Martinez-Gomez P, Dicenta F, Audergon JM (2000) Behaviour of apricot (Prunus armeniaca L.) cultivars in the presence of sharka (plum pox potyvirus): a review. Agronomie 20:407–422
Mercado JA, El Mansouri I, Jimenez-Bermudez S, Plieco-Alfaro F, Quesada MA (1999) A convenient protocol for extraction and purification of DNA from Fragaria. In Vitro Cell Dev Biol Plant 35:152–153
Messina R, Lain O, Marrazzo MT, Cipriani G, Testolin R (2004) New set of microsatellite loci isolated in apricot. Mol Ecol Notes 4:432–434
Rajapakse S, Belthoff LE, He G, Estager A-E, Scorza R, Verde I, Ballard RE, Baird WV, Callahan A, Monet R, Abbott AG (1995) Genetic mapping in peach using morphological, RFLP and RAPD markers. Theor Appl Genet 90:503–510
Sosinski B, Gannavarapu M, Hager LD, Beck LE, King GJ, Ryder CD, Rajapakse S, Baird WV, Ballard RE, Abbott AG (2000) Characterization of microsatellite markers in peach (Prunus persica L. Batsch). Theor Appl Genet 101:421–428
Testolin R, Marrazzo MT, Cipriani G, Quarta R, Verde I, Dettori MT, Pancaldi M, Sansavini S (2000) Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars. Genome 43:512–520
Testolin R, Messina R, Lain O, Marrazzo MT, Huang WG, Cipriani G (2004) Microsatellites isolated in almond from an AC-repeat enriched library. Mol Ecol Notes 4:459–461
van Ooijen JW, Voorrips RW (2002) Joinmap 3.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen
Verde I, Lauria M, Dettori MT, Vendramin E, Balconi C, Micali S, Wang Y, Marrazzo MT, Cipriani G, Hartings H, Testolin R, Abbott AG, Motto M, Quarta R (2005) Microsatellite and AFLP markers in the Prunus persica [L. (Batsch)] × P. ferganensis BC1 linkage map: saturation and coverage improvement. Theor Appl Genet 111:1013–1021
Vilanova S, Romero C, Abbott AG, Llacer G, Badenes ML (2003) An apricot (Prunus armeniaca L.) F2 progeny linkage map based on SSR and AFLP markers mapping plum pox virus resistance and self-incompatibility traits. Theor Appl Genet 107:239–247
Viruel MA, Messeguer R, de Vicente MC, Garcia-Mas J, Puigdomenech P, Vargas F, Arús P (1995) A linkage map with RFLP and isoenzyme markers for almond. Theor Appl Genet 91:964–971
Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLS. J Hered 93:77–78
Wang D, Karle R, Brettin TS, Iezzoni AF (1998) Genetic linkage map in sour cherry using RFLP markers. Theor Appl Genet 97:1217–1224
Yamamoto T, Takehiko S, Tsuyoshi I, Yaegaki H, Haji T, Matsuta N, Yamaguchi M, Hayashi T (2001) Characterization of morphological traits based on a genetic linkage map in peach. Breed Sci 51:271–278
Acknowledgements
This research work was funded by the Italian Ministry of University, Research and Technology in the framework of project PRIN 2003 ‘Resistance to PPV (Plum Pox Virus) in apricot: fine mapping of genes/QTLs and production of resistant breeding lines’.
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Communicated by P. Arús
An erratum to this article is available at http://dx.doi.org/10.1007/s11295-006-0079-4.
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Dondini, L., Lain, O., Geuna, F. et al. Development of a new SSR-based linkage map in apricot and analysis of synteny with existing Prunus maps. Tree Genetics & Genomes 3, 239–249 (2007). https://doi.org/10.1007/s11295-006-0059-8
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DOI: https://doi.org/10.1007/s11295-006-0059-8