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Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae

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Abstract

A number of members of the SAP (“stress-associated protein”) gene family have been implicated in the plant stress response. Here, a SAP gene has been isolated using PCR RACE from the perennial grass Leymus chinensis, a species which has reputation for ecological adaptability. The 17.6 kDa LcSAP product comprised 161 residues, including both an A20 domain and an AN1 domain, a feature of type I SAPs. Using a semi-quantitative RT-PCR assay to profile its transcription, it was shown that LcSAP was more strongly transcribed in the leaf than in the root under control conditions. The level of LcSAP transcription began to rise 6 h after the plant’s exposure to 400 mM NaCl, and the abundance of transcript remained stable for at least 24 h. Exposing the plant to 100 mM Na2CO3 also induced LcSAP transcription, but the abundance of SAP transcript faded after 6 h. When LcSAP was introduced into yeast cells, the transgenic cells grew better than wild type ones when the medium contained 1.4 M NaCl. The ability of LcSAP to respond to salinity stress in yeast suggests that it also makes a contribution to the stress tolerance shown by L. chinensis.

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References

  1. Giri J, Dansana PK, Kothari KS, Sharma G, Vij S et al (2013) SAPs as novel regulators of abiotic stress response in plants. Bioessays 35:639–648

    Article  CAS  PubMed  Google Scholar 

  2. Kim GD, Cho YH, Yoo SD (2015) Regulatory functions of evolutionarily conserved AN1/A20-like Zinc finger family proteins in Arabidopsis stress responses under high temperature. Biochem Biophys Res Commun 457:213–220

    Article  CAS  PubMed  Google Scholar 

  3. Dansana PK, Kothari KS, Vij S, Tyagi AK (2014) OsiSAP1 overexpression improves water-deficit stress tolerance in transgenic rice by affecting expression of endogenous stress-related genes. Plant Cell Rep 33:1425–1440

    Article  CAS  PubMed  Google Scholar 

  4. Hozain M, Abdelmageed H, Lee J, Kang M, Fokar M et al (2012) Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress. J Plant Physiol 169:1261–1270

    Article  CAS  PubMed  Google Scholar 

  5. Tyagi H, Jha S, Sharma M, Giri J, Tyagi AK (2014) Rice SAPs are responsive to multiple biotic stresses and overexpression of OsSAP1, an A20/AN1 zinc-finger protein, enhances the basal resistance against pathogen infection in tobacco. Plant Sci 225:68–76

    Article  CAS  PubMed  Google Scholar 

  6. Diatchenko L, Romanov S, Malinina I, Clarke J, Tchivilev I et al (2005) Identification of novel mediators of NF-kappa B through genome-wide survey of monocyte adherence-induced genes. J Biol Chem 78:1366–1377

    CAS  Google Scholar 

  7. Dixit VM, Green S, Sarma V, Holzman LB, Wolf FW et al (1990) Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin. J Biol Chem 265:2973–2978

    CAS  PubMed  Google Scholar 

  8. Opipari AWJ, Boguski MS, Dixit VM (1990) The A20 cDNA induced by tumor necrosis factor alpha encodes a novel type of zinc finger protein. J Biol Chem 265:14705–14708

    CAS  PubMed  Google Scholar 

  9. Rebagliati MR, Weeks DL (1985) Identification and cloning of localized maternal RNAs from Xenopus eggs. Cell 42:769–777

    Article  CAS  PubMed  Google Scholar 

  10. Jin Y, Wang M, Fu J, Xuan N, Zhu Y et al (2007) Phylogenetic and expression analysis of ZnF-AN1 genes in plants. Genomics 90:265–275

    Article  CAS  PubMed  Google Scholar 

  11. Vij S, Tyagi AK (2006) Genome-wide analysis of the stress associated protein (SAP) gene family containing A20/AN1 zinc-finger(s) in rice and their phylogenetic relationship with Arabidopsis. Mol Genet Genomics 276:565–575

    Article  CAS  PubMed  Google Scholar 

  12. Solanke AU, Sharma MK, Tyagi AK, Sharma AK (2009) Characterization and phylogenetic analysis of environmental stress-responsive SAP gene family encoding A20/AN1 zinc finger proteins in tomato. Mol Genet Genomics 282:153–164

    Article  CAS  PubMed  Google Scholar 

  13. Vij S, Tyagi AK (2008) A20/AN1 zinc-finger domain-containing proteins in plants and animals represent common elements in stress response. Funct Integr Genomics 8:301–307

    Article  CAS  PubMed  Google Scholar 

  14. Takatsuji H (1998) Zinc finger transcription factors in plants. Cell Mol Life Sci 54:582–596

    Article  CAS  PubMed  Google Scholar 

  15. Jin H, Plaha P, Park JY, Hong CP, Lee IS et al (2006) Comparative EST profiles of leaf and root of Leymus chinensis, a xerophilous grass adapted to high pH sodic soil. Plant Sci 170:1081–1086

    Article  CAS  Google Scholar 

  16. Ma P, Liu J (2012) Isolation and characterization of a novel plasma membrane intrinsic protein gene, LcPIP1, in Leymus chinensis that enhances salt stress tolerance in Saccharomyces cerevisiae. Appl Biochem Biotechnol 166:479–485

    Article  CAS  PubMed  Google Scholar 

  17. Huang J, Wang MM, Jiang Y, Bao YM, Huang X et al (2008) Expression analysis of rice A20/AN1-type zinc finger genes and characterization of ZFP177 that contributes to temperature stress tolerance. Gene 420:135–144

    Article  CAS  PubMed  Google Scholar 

  18. Kang M, Fokar M, Abdelmageed H, Allen RD (2011) Arabidopsis SAP5 functions as a positive regulator of stress responses and exhibits E3 ubiquitin ligase activity. Plant Mol Biol 75:451–466

    Article  CAS  PubMed  Google Scholar 

  19. Dixit AR, Dhankher OP (2011) A novel stress-associated protein ‘AtSAP10’ from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress. PLoS One 6:e20921

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Wang Y, Zhang L, Zhang L, Xing T, Peng J et al (2013) A novel stress-associated protein SbSAP14 from Sorghum bicolor confers tolerance to salt stress in transgenic rice. Mol Breed 32:437–449

    Article  CAS  Google Scholar 

  21. Paul A, Kumar S (2015) An A20/AN1-zinc-finger domain containing protein gene in tea is differentially expressed during winter dormancy and in response to abiotic stress and plant growth regulators. Plant Gene 1:1–7

    Article  CAS  Google Scholar 

  22. Li XJ, Wu YL, Yang BP, Wang JG, Zhang SZ et al (2014) Function analysis of sugarcane A20/AN1 zinc-finger protein gene ShSAP1 in transgenic tobacco. Crop Sci 54:2724–2734

    Article  Google Scholar 

  23. Saad RB, Zouari N, Ramdhan WB, Azaza J, Meynard D et al (2010) Improved drought and salt stress tolerance in transgenic tobacco overexpressing a novel A20/AN1 zinc-finger “AlSAP” gene isolated from the halophyte grass Aeluropus littoralis. Plant Mol Biol 72:171–190

    Article  PubMed  Google Scholar 

  24. Mukhopadhyay A, Vij S, Tyagi AK (2004) Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proc Natl Acad Sci USA 1:6309–6314

    Article  Google Scholar 

  25. Kanneganti V, Gupta AK (2008) Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice. Plant Mol Biol 66:445–462

    Article  CAS  PubMed  Google Scholar 

  26. Gimeno GC, Gervais ML, Planchet E, Satour P, Limami AM et al (2011) A stress-associated protein containing A20/AN1 zing-finger domains expressed in Medicago truncatula seeds. Plant Physiol Biochem 43:3303–3310

    Google Scholar 

  27. Martin RC, Glover CK, Baldwin JC, Dombrowski JE (2012) Identification and characterization of a salt stress-inducible zinc finger protein from Festuca arundinacea. BMC Res Notes 5:66

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Sreedharan S, Shekhawat UK, Ganapathi TR (2012) MusaSAP1, a A20/AN1 zinc finger gene from banana functions as a positive regulator in different stress responses. Plant Mol Biol 80:503–517

    Article  CAS  PubMed  Google Scholar 

  29. Giri J, Vij S, Prasant K, Dansana PK, Tyagi AK (2011) Rice A20/AN1 zinc-finger containing stress-associated proteins (SAP1/11) and a receptor-like cytoplasmic kinase (OsRLCK253) interact via A20 zinc-finger and confer abiotic stress tolerance in transgenic Arabidopsis plants. New Phytol 191:721–732

    Article  CAS  PubMed  Google Scholar 

  30. Charrier A, Planchet E, Cerveau D, Gimeno GC, Verdu I et al (2012) Overexpression of a Medicago truncatula stress-associated protein gene (MtSAP1) leads to nitric oxide accumulation and confers osmotic and salt stress tolerance in transgenic tobacco. Planta 236:567–577

    Article  CAS  PubMed  Google Scholar 

  31. Charrier A, Lelièvre E, Limami AM, Planchet E (2013) Medicago truncatula stress associated protein 1 gene (MtSAP1) overexpression confers tolerance to abiotic stress and impacts proline accumulation in transgenic tobacco. J Plant Physiol 170:74–877

    Article  Google Scholar 

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Acknowledgements

This work was supported by Chinese Universities Scientific Fund (2452016006, 2014YB044 and 2452016006), Specialized Research Fund for the Doctoral Program of Higher Education (20130204120018 and 20130204120038), Natural Science Basic Research Plan in Shaanxi Province of China (2013JQ3006), National Natural Science Foundation of China (31401839 and 31670295), Young Talent Funding (A314021402-1522) provided by the Institute of Soil and Water Conservation, NWAFU.

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    Authors and Affiliations

    1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, 712100, People’s Republic of China

      Jingying Liu

    2. College of Life Sciences, Northwest A&F University, Yangling, 712100, People’s Republic of China

      Jingying Liu, Xiangna Yang, Xizhe Yang, Mingyue Xu, Jie Liu, Mengmeng Xue & Pengda Ma

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    1. Jingying Liu
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    Correspondence to Pengda Ma.

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    Jingying Liu and Xiangna Yang have contributed equally to this work.

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    Liu, J., Yang, X., Yang, X. et al. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae . Mol Biol Rep 44, 5–9 (2017). https://doi.org/10.1007/s11033-016-4091-y

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