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Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice

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Abstract

We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submergence, wounding, heavy metals as well as stress hormone Abscisic acid. OsiSAP8 protein fused to GFP was localized towards the periphery of the cells in the epidermal cells of infiltrated Nicotiana benthamiana leaves. Yeast two hybrid analysis revealed that A20 and AN1 type zinc-finger domains of OsiSAP8 interact with each other. Overexpression of the gene in both transgenic tobacco and rice conferred tolerance to salt, drought and cold stress at seed germination/seedling stage as reflected by percentage of germination and gain in fresh weight after stress recovery. Transgenic rice plants were tolerant to salt and drought during anthesis stage without any yield penalty as compared to unstressed transgenic plants.

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Acknowledgements

We would like to thank Dr Akhilesh. K. Tyagi, Delhi University South campus, India for providing us the rice root cDNA library, Dr J. J. Finer, Ohio state university, USA for providing pHX4 vector and Dr Yedidya Gafni, Agricultural Research Organization, Israel for helping in sub-cellular localization studies. Special thanks go to Eddy (ARO, Israel) for technical assistance with confocal microscopy. AKG likes to acknowledge the financial support from Department of Biotechnology (DBT)-India, Centre for potential for genomic sciences-University grants commission (CPSGS-UGC) and UGC-SAP programmes. K.V is the recipient of a research fellowship from the Council for Scientific and Industrial Research.

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  1. Vydehi Kanneganti

    Present address: Department of Genetics, Agricultural Research Organization, The Volcani Center, Post Box 6, Bet Dagan, 50250, Israel

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  1. Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625021, TamilNadu, India

    Vydehi Kanneganti & Aditya Kumar Gupta

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  1. Vydehi Kanneganti
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Correspondence to Aditya Kumar Gupta.

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OsiSAP8 is deposited in the Genbank with the Accession number AY345599.

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Kanneganti, V., Gupta, A.K. Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice. Plant Mol Biol 66, 445–462 (2008). https://doi.org/10.1007/s11103-007-9284-2

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