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Genome mining for lasso peptides: past, present, and future

  • Natural Products - Review
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Over the course of roughly a decade, the lasso peptide field has been transformed. Whereas new compounds were discovered infrequently via activity-driven approaches, now, the vast majority of lasso peptide discovery is driven by genome-mining approaches. This paper starts with a historical overview of the first genome-mining approaches for lasso peptide discovery, and then covers new tools that have emerged. Several examples of novel lasso peptides that have been discovered via genome mining are presented as are examples of new enzymes found associated with lasso peptide gene clusters. Finally, this paper concludes with future directions and unsolved challenges in lasso peptide genome mining.

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Acknowledgements

Lasso peptide research in the Link Lab is supported by the NIH (GM107036) and a grant from the Princeton University School of Engineering and Applied Sciences (SEAS) for the Focused Research Team on Precision Antibiotics.

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  1. Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, 08544, USA

    Wai Ling Cheung-Lee & A. James Link

  2. Department of Chemistry, Princeton University, Princeton, NJ, 08544, USA

    A. James Link

  3. Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA

    A. James Link

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Cheung-Lee, W.L., Link, A.J. Genome mining for lasso peptides: past, present, and future. J Ind Microbiol Biotechnol 46, 1371–1379 (2019). https://doi.org/10.1007/s10295-019-02197-z

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