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Isolation and identification of a new lasso peptide cattlecin from Streptomyces cattleya based on genome mining

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Abstract

Lasso peptides are ribosomally synthesized and posttranslationally modified peptides with diverse biological functions. Recent genome mining has revealed that many species of actinomycetes possibly contain biosynthetic gene clusters of lasso peptides. With genome mining for lasso peptide biosynthesis, we screened several actinomycetes for lasso peptide production using high-performance liquid chromatography and electrospray ionization–mass spectrometry. Consequently, Streptomyces cattleya was identified as a producer of a new lasso peptide named cattlecin. Analysis of amino acid content on cattlecin indicated the presence of four moles each of Asp and His, three moles each of Gly and Tyr, and one mole of Ser. Tandem mass spectrometry (MS/MS) analysis of cattlecin revealed C-terminal sequence of WHHGWYGWWDD. The peptide sequence (SYHWGDYHDWHHGWYGWWDD) was the expected amino acid sequence of cattlecin based on genome mining. As a result of MS/MS analysis, the amine residue of the first Ser was proposed to form a macrolactam ring with the β-carboxyl residue of the ninth Asp. The biosynthetic gene cluster of cattlecin comprised four genes: catA, catC, catB1, and catB2, which is typical of a lasso peptide biosynthetic gene cluster in actinomycetes.

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Acknowledgment

This study was supported by the Japan Society for the Promotion of Science by Grants-in-Aid (Grant no. 25350964).

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Authors and Affiliations

  1. Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Shogo Sugai & Shinya Kodani

  2. Food Research Institute, NARO, 2-1-12 Kan-nondai, Tsukuba, Ibaraki, 305-8642, Japan

    Mayumi Ohnishi-Kameyama

  3. College of Agriculture, Academic Institute, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Shinya Kodani

  4. Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Shinya Kodani

Authors
  1. Shogo Sugai
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  2. Mayumi Ohnishi-Kameyama
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  3. Shinya Kodani
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Correspondence to Shinya Kodani.

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Sugai, S., Ohnishi-Kameyama, M. & Kodani, S. Isolation and identification of a new lasso peptide cattlecin from Streptomyces cattleya based on genome mining. Appl Biol Chem 60, 163–167 (2017). https://doi.org/10.1007/s13765-017-0268-x

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  • DOI: https://doi.org/10.1007/s13765-017-0268-x

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