Summary
A plasmid library of chloroplast (Cp) DNA fromChlamydomonas reinhardtii was used to screen for transcripts which respond to light. A transcript of R03, a 1,300 byEcoRI fragment, was identified as a message which accumulates in darkness. The transcribed region of R03 showed extensive sequence homology with theEscherichia coli elongation factor gene,tufA. A genespecific probe was constructed. Northern blots were used to study the extent and kinetics of accumulation of this transcript in darkness and in the presence of antibiotic inhibitors of Cp ribosomes. For comparison, the effects of darkness and antibiotics on the steady state levels ofpsbA, rbeL, and 16S rRNA were also studied. We conclude that thetuf transcript shows the greatest increase in darkness and in the presence of antibiotics.
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References
Ambulos NP, Duvall EJ, Lovett PS (1987) Gene 51:281–286
Blamire J, Flechtner VR, Sager R (1971) Proc Natl Acad Sci USA 71:2867–2871
Chua NH, Bloebel G, Siekevitz P, Palada GE (1976) J Cell Biol 71:497–514
Chiang K-S, Sueoka N (1967) Proc Natl Sci USA 57:1506–1513
Dorman L, Carvill EP, Gilliland TJ, John PC (1985) New Phytol 99:1–40
Dron M, Rahire M, Rochaix J-D (1982a) J Mol Biol 162:775–793
Dron M, Rahire M, Rochaix J-D (1982b) Nucleic Acids Res 10:7609–7620
Erickson JM, Rahire M, Rochaix J-D (1984) EMBO J 3:2753–2762
Glisin V, Crkvenjakov C, Byus C (1974) Biochemistry 13:2633–2637
Grant D, Swinton DC, Chiang K-S (1978) Planta 141:259–267
Hanahan D (1983) J Mol Biol 166:557–580
Hoober JK (1984) Chloroplasts. Plenum Press, NY
Kaziro Y (1978) Biochem Biophys Acta 505:95–127
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Matsuda Y, Surzycki SJ (1980) Mol Gen Genet 180:463–474
McAteer M, Donnan L, John PC (1984) New Phytol 99:42–56
Rochaix J-D (1978) J Mol Biol 126:597–617
Rochaix J-D (1981) Experentia 37:323–340
Schloss JA, Siflow CD, Rosenbaum JL (1984) Mol Cell Biol 4:424–432
Surzycki S (1970) Methods Enzymol 22:67–70
Thompson RJ, Mosig G (1984) Plant Physiol 76:1–6
Thompson RJ, Mosig G (1987) Cell 48:281–287
Van Delft JHM, Schmidt DS, Bosch L (1987) J Mol Biol 197:647–657
Vazquez D (1979) Inhibitors of protein synthesis. Springer, Berlin Heidelberg New York
Watson JC, Surzycki SJ (1982) Proc Natl Acad Sci USA 79:2264–2267
Woessner JP, Gillham NW, Boyton JE (1987) Plant Mol Biol 8:151–158
Yokota T, Sugisaki H, Takanami M, Kaziro Y (1980) Gene 12:25–31
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Silk, G.W., Wu, M. Darkness and antibiotics increase the steady-state transcripts of the elongation factor gene (tuf) inChlamydomonas reinhardtii . Curr Genet 14, 119–126 (1988). https://doi.org/10.1007/BF00569335
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DOI: https://doi.org/10.1007/BF00569335