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Cell Culture Methods

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Abstract

The restoration of osteochondral defects presents great challenges that have not been fully solved by the current therapies. Therefore, this field continues to expand, bridging the gap between palliative care and defects reconstruction. In the last few years, tissue engineering and regenerative medicine have been offering advanced strategies and some of which have successfully reached clinical application and the market. Beyond the origin and source of cells, the development of culture conditions remains an important step to further clinical applications. Several approaches have been focused on good manufacturing practice (GMP) conditions. The aim is the creation of advanced therapy medicinal products (ATMPs). The up-to-date state of the culture protocols for osteochondral tissue engineering with respect to different cells, growth factors, and biomaterial scaffolds, as well as the strategies employed in clinical trials for the restoration and repair of osteochondral defects, will be the focus of this book chapter.

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Author information

Author notes

  1. Alain da Silva Morais and Fátima Raquel Maia contributed equally to this work.

Authors and Affiliations

  1. 3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark—Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco GMR, 4805-017, Portugal

    Alain da Silva Morais, F. Raquel Maia, Rui L. Reis & Joaquim M. Oliveira

  2. ICVS/3B’s—PT Government Associate Laboratory, Braga, Portugal

    Alain da Silva Morais, F. Raquel Maia, Rui L. Reis & Joaquim M. Oliveira

Authors
  1. Alain da Silva Morais
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  2. F. Raquel Maia
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  3. Rui L. Reis
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  4. Joaquim M. Oliveira
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Corresponding authors

Correspondence to Alain da Silva Morais or F. Raquel Maia .

Editor information

Editors and Affiliations

  1. O.A.S.I. Bioresearch Foundation NPO, Milan, Italy

    Alberto Gobbi

  2. Clínica do Dragão, Espregueira-Mendes Sports Centre – FIFA Medical Centre of Excellence, Porto, Portugal

    João Espregueira-Mendes

  3. Musculoskeletal and Joint Research Foundation, San Diego, California, USA

    John G. Lane

  4. Department of Orthopedics, Acibadem University, Istanbul, Turkey

    Mustafa Karahan

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© 2017 ISAKOS

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da Silva Morais, A., Maia, F.R., Reis, R.L., Oliveira, J.M. (2017). Cell Culture Methods. In: Gobbi, A., Espregueira-Mendes, J., Lane, J., Karahan, M. (eds) Bio-orthopaedics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-54181-4_49

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  • DOI: https://doi.org/10.1007/978-3-662-54181-4_49

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-54180-7

  • Online ISBN: 978-3-662-54181-4

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