Abstract
2,4,6-Trinitrotoluene (TNT) is a prominent environmental pollutant due to its widespread use in military, industrial, and mining activities. Numerous documented physical and chemical abiotic treatment technologies for its disposal exist, but microbial remediation emerged as a green substitute for TNT disposal administration proficiently and ecologically. This chapter systematically examines the biotic conversion and diminution of TNT through microbial degradation, focusing on bacterial breakdown and the synergistic interaction of microbes and TNT in this process. We analyze the biochemical pathways and mechanisms employed by various bacteria in TNT degradation, highlighting the role of enzymes and metabolic intermediates. This chapter aims to provide a detailed discussion of TNT attenuation through microbes in both aerobic and anaerobic environments in the aqueous and soil strata. Additionally, it comprehends the strength of microbial strains in isolation, consortium, and under immobilized conditions. Furthermore, the synergistic interaction of microbes is explored, emphasizing their potential for accelerating the biotransformation of TNT. Additionally, we discuss the factors influencing bacterial degradation. This chapter focuses on the comprehensive discussion of TNT attenuation through microbial remediation, covering degradation mechanisms, microbial strains, and treatment arrangements.
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References
Agency for Toxic Substances and Disease Registry (ATSDR) (1995) Toxicological Profile for 2,4,6-Trinitrotoluene (TNT). www.atsdr.cdc.gov/toxprofiles/TP.asp?id=677&tid=125
Alexander M (1999) Biodegradation and bioremediation, 2nd. Academic Press, London, England
Alnaizy R, Akgerman A (1999) Oxidative treatment of high explosives contaminated wastewater. Water Res 33(9):2021–2030. https://doi.org/10.1016/S0043-1354(98)00424-2
Alothman ZA, Bahkali AH, Elgorban AM, Al-Otaibi MS, Ghfar AA, Gabr SA, Ahmed AYBH (2020) Bioremediation of explosive TNT by trichoderma viride. Molecules 25(6):1393. https://doi.org/10.3390/molecules25061393
Alvarez MA, Kitts CL, Botsford JL, Unkefer PJ (1995) Pseudomonas aeruginosa strain MA01 aerobically metabolizes the amino-di-nitrotoluenes produced by 2,4,6-Trinitrotoluene nitro group reduction. Can J Microbiol 41:984–991. http://www.ncbi.nlm.nih.gov/pubmed/7497356
Anand S, Celin SM, Bhalla R, Sahai S, Hooda L (2016) Bioremediation potential of Pseudomonas sp. isolated from a 2,4,6-trinitrotoulene contaminated site. Int J Environ Waste Manag 18(2):145. https://doi.org/10.1504/IJEWM.2016.080401
Bodeau D (1993) Occupational health: the soldier and the industrial base, Chapter 9, Military energetic materials: explosives and propellant
Boopathy R, Kulpa CF (1992) Trinitrotoluene (TNT) as a sole nitrogen source for a sulfate-reducing bacterium Desulfovibrio sp. (B strain) isolated from an anaerobic digester. Curr Microbiol 25(4):235–241. http://www.ncbi.nlm.nih.gov/pubmed/1368976
Boopathy R, Kulpa CF (1994) Biotransformation of 2, 4, 6-trinitrotoluene (TNT) by a Methanococcus sp (strain B) isolated from a lake sediment. Can J Microbiol 40(4):273–278. http://www.ncbi.nlm.nih.gov/pubmed/8039051
Boopathy R, Wilson M, Kulpa CF (1993) Anaerobic removal of 2,4,6-trinitrotoluene (TNT) under different electron accepting conditions: laboratory study. Water Environ Res 65(3): 271–275. http://www.jstor.org/stable/25044298
Bordeleau G, Martel R, Ampleman G, Thiboutot S (2013) Photolysis of RDX and nitroglycerin in the context of military training ranges. Chemosphere 93(1):14–19. https://doi.org/10.1016/j.chemosphere.2013.04.048
Brentner LB, Mukherji ST, Merchie KM, Yoon JM, Schnoor JL, Aken BV (2008) Expression of glutathione S-transferases in poplar trees (Populustrichocarpa) exposed to 2, 4, 6-trinitrotoluene (TNT). Chemosphere 73(5):657–662. https://doi.org/10.1016/j.chemosphere.2008.07.059
Bui ND, Minh TT (2021) Investigation of TNT red wastewater treatment technology using the combination of advanced oxidation processes. Sci Total Environ 756:143852. https://doi.org/10.1016/j.scitotenv.2020.143852
Burken JG, Shanks JV, Thompson PL (2000) Phytoremediation and plant metabolism of explosives and nitroaromatic compounds. In: Spain JC, Hughes JB, Knackmuss H (eds) Biodegradation of nitroaromatic compounds and explosives. CRC Press, Boca Raton, Florida, pp 239–276
Caballero A, Lázaro JJ, Ramos JL, Esteve-Núñez A (2005) PnrA, a new nitroreductase-family enzyme in the TNT-degrading strain Pseudomonas putida JLR11. Environ Microbiol 7:1211–1219. https://doi.org/10.1111/j.1462-2920.2005.00801.x
Cabrera ÁM, Márquez LS, Osorio IM, Castro-Nallar E, González-Nilo DF, Pérez-Donoso MJ (2020) Biotransformation of 2,4,6-trinitrotoluene by Pseudomonas sp. TNT3 isolated from Deception Island, Antarctica. Environ Pollut 262:113922. https://doi.org/10.1016/j.envpol.2020.113922
Cai X, Servinsky M, Kiel J, Sund C, Bennett GN (2013) Analysis of redox responses during TNT transformation by Clostridium acetobutylicum ATCC 824 and mutants exhibiting altered metabolism. Appl Microbiol Biotechnol 97:4651–4663. https://doi.org/10.1007/s00253-012-4253-3
Chatterjee S, Deb U, Datta S, Walther C, Gupta DK (2017) Common explosives (TNT, RDX, HMX) and their fate in the environment: emphasizing bioremediation. Chemosphere 184:438–451. https://doi.org/10.1016/j.chemosphere.2017.06.008
Chaudhary DK, Kim J (2019) New insights into bioremediation strategies for oil-contaminated soil in cold environments. Int Biodeterior Biodegradation 142:58–72. https://doi.org/10.1016/j.ibiod.2019.05.001
Claus H, Bausinger T, Lehmler I, Perret N, Fels G, Dehner U, Preuß J, König H (2007) Transformation of 2,4,6- trinitrotoluene (TNT) by Raoultella. Biodegradation 18(1):27–35. https://doi.org/10.1007/s10532-005-9033-7
Claus H (2014) Microbial degradation of 2,4,6- Trinitrotoluene in vitro and in natural environments, In: Singh SN (ed) Biological remediation of explosive residues, environmental science and engineering. Springer International Publishing, pp 15–38. https://doi.org/10.1007/978-3-319-01083-0_2
Crawford R (1995) The microbiology and treatment of nitroaromatic compounds. Curr Opin Biotechnol 6(1995):329–336. https://doi.org/10.1016/0958-1669(95)80055-7
Cupples AM (2013) RDX degrading microbial communities and the prediction of microorganisms responsible for RDX bioremediation. Int Biodeterior Biodegradation 85:260–270. https://doi.org/10.1016/j.ibiod.2013.08.002
Drzyzga O, Bruns-Nagel D, Gorontzy T, Blotevogel KH, Gemsa D (1998) Mass balance studies with 14C-labeled 2,4,6-trinitrotoluene (TNT) mediated by an Anaerobic desulfovibrio species and an Aerobic serratia species. Curr Microbiol 37:380–386. http://www.ncbi.nlm.nih.gov/pubmed/9806975
Duque E, Haidour A, Godoy F, Ramos LJ (1993) Construction of a Pseudomonas hybrid strain that mineralizes 2,4,6- trinitrotoluene. J Bacteriol 175(8):2278–2283. https://doi.org/10.1128/jb.175.8.2278-2283.1993
Ederer MM, Lewis TA, Crawford RL (1997) 2, 4, 6-Trinitrotoluene (TNT) transformation by clostridia isolated from a munition-fed bioreactor: comparison with non-adapted bacteria. J Ind Microbiol Biotechnol 18(2–3):82–88. https://doi.org/10.1038/sj.jim.2900257
EPA (2013) Superfund information systems, superfund site information. www.epa.gov/superfund/sites/cursites/
Esteve-Nunez A, Caballero A, Ramos JL (2001) Biological degradation of 2,4,6-trinitrotoluene. Microbiol Mol Biol Rev 65(3):335–352. https://doi.org/10.1128/mmbr.65.3.335-352.2001
Fiorella PD, Spain JC (1997) Transformation of 2,4,6-trinitrotoluene by Pseudomonas pseudoalcaligenes JS52. Appl Microbiol Biotechnol 63:2007–2015. https://doi.org/10.1128/aem.63.5.2007-2015.1997
French CE, Nicklin S, Bruce NC (1998) Aerobic degradation of 2,4,6-trinitrotoluene by Enterobacter cloacae PB2 and by pentaerythritol tetranitrate reductase. Appl Environ Microbiol 64(8):2864–2868. https://doi.org/10.1128/AEM.64.8.2864-2868.1998.PMID:9687442;PMCID:PMC106784
Gad CS (2014) Trinitrotoluene, Encyclopedia of. Toxicology 4:855–857. https://doi.org/10.1016/b978-0-12-386454-3.00957-X
Gilcrease PC, Murphy VG (1995) Bioconversion of 2,4-diamino-6-nitrotoluene to a novel metabolite under anoxic and aerobic conditions. Appl Microbiol Biotechnol 61:4209–4214. https://doi.org/10.1128/aem.61.12.4209-4214.1995,CorpusID:12700897
Gök ZG, Inal M, Yiğitoğlu M (2018) Effective degradation of 2,4,6-trinitrotoluene (TNT) with a bacterial consortium developed from high TNT-degrading bacteria Isolated from TNT-contaminated soil. Hacettepe J Biol Chem 45(3):445–455. https://doi.org/10.15671/HJBC.2018.252
Gumuscu B, Cekmecelioglu D, Tekinay T (2015) Complete dissipation of 2,4,6-trinitrotoluene by in-vessel composting. RSC Adv 5(64):51812–51819. https://doi.org/10.1039/C5RA07997G
Gunning V, Tzafestas K, Sparrow H, Johnston EJ, Brentnall AS, Potts JR, Rylott EL, Bruce NC (2014) Arabidopsis glutathione transferases U24 and U25 exhibit a range of detoxification activities with the environmental pollutant and explosive, 2, 4, 6-trinitrotoluene. Plant Physiol 165(2):854–865. https://doi.org/10.1104/pp.114.237180
Gupta S, Siebner H, Ramanathan G, Ronen Z (2022) Inhibition effect of 2,4,6-trinitrotoluene (TNT) on RDX degradation by rhodococcus strains isolated from contaminated soil and water. Environ Pollut 311:2022. https://doi.org/10.1016/j.envpol.2022.120018
Gupta S, Goel SS, Siebner H, Ronen Z, Ramanathan G (2023) Transformation of 2, 4, 6-trinitrotoluene by Stenotrophomonas strain SG1 under aerobic and anaerobic conditions. Chemosphere 311(1).https://doi.org/10.1016/j.chemosphere.2022.137085
HaÏdour A, Ramos JL (1996) Identification of products resulting from the biological reduction of 2,4,6-trinitrotoluene, 2,4-dinitrotoluene, and 2,6-dinitrotoluene by Pseudomonas spp. Environ Sci Technol 30(7):2365–2370. https://doi.org/10.1021/es950824u
Hannink NK, Rosser SJ, Bruce NC (2002) Phytoremediation of explosives. Crit Rev Plant Sci 21(5):511–538. https://doi.org/10.1080/0735-260291044340
Hawari J (2000) Biodegradation of RDX and HMX: from basic research to field application In: biodegradation of nitroaromatic by compounds and explosives. Biodegrad Nitroaromatic Compd Explos 277–310. https://doi.org/10.1201/9781420032673.ch11
Higson FK (1992) Microbial degradation of nitroaromatic compounds. Adv Appl Microbiol 37:1–19. https://doi.org/10.1016/s0065-2164(08)70250-8
Hinshaw GD, Fansk CB, Fiscus DE, Sorenson SA (1987) Granular activated carbon (GAC) system performance and capabilities and optimization, Report AMXTH-TE-GR87111, U.S. Army Toxic and Hazardous Materials Agency, Aberdeen Proving Ground, MD, U.S
HSDB (2012) 2,4,6-Trinitrotoluene (TNT), www.atsdr.cdc.gov/toxprofiles/TP.asp?id=677&tid=125. Hazardous Substances Data Bank (HSDB)
Jones AM, Greer CW, Hawari J, Ampleman G, Lavigne J (1995) Biodegradability of selected highly energetic pollutants under aerobic conditions. http://www.osti.gov/scitech/biblio/490985-biodegradability-selected-highly-energetic-pollutants-under-aerobic-conditions
Kalafut T, Wales ME, Rastogi VK, Naumov PR, Zaripova KS, Wild RJ (1998) Biotransformation patterns of 2,4,6-trinitrotoluene by aerobic bacteria. Curr Microbiol 36:45–54. https://doi.org/10.1007/s002849900278
Kalderis D, Juhasz LA, Boopathy R, Comfort S (2011) Soils contaminated with explosives: environmental fate and evaluation of state-of-the-art remediation processes (IUPAC Technical Report). Pure Appl Chem 83(7):1407–1484. https://doi.org/10.1351/PAC-REP-10-01-05
Kao C-M, Lin B-H, Chen S-C, Wei S-F, Chen C-C, Yao C-L, Chien C-C (2016) Biodegradation of trinitrotoluene (TNT) by indigenous microorganisms from TNT-contaminated soil, and their application in TNT bioremediation. Bioremediat J 1–9.https://doi.org/10.1080/10889868.2016.1148007
Kim HY, Song HG (2003) Transformation and mineralization of 2,4,6-trinitrotoluene by the white rot fungus Irpex lacteus. Appl Microbiol Biotechnol 61:150–156. https://doi.org/10.1007/s00253-002-1211-5
Kim HY, Song HG (2005) Purification and characterization of NAD(P)H-dependent nitroreductase I from Klebsiella sp. C1 and enzymatic transformation of 2,4,6-trinitrotoluene. Appl Microbiol Biotechnol 68:766–773. https://doi.org/10.1007/s00253-005-1950-1
Kirk-Othmer RE (1993) Kirk-Othmer encyclopedia of chemical technology, chlorocarbons and chlorohydrocarbons-CSUB 2/SUB to combustion technology
Koske D, Goldenstein IN, Kammann U (2019) Nitroaromatic compounds damage the DNA of zebrafish embryos (Danio rerio). Aquat Toxicol 217. https://doi.org/10.1016/j.aquatox.2019.105345
Koutsospyros A, Pavlov J, Fawcett J, Strickland D, Smolinski B, Braida W (2012) Degradation of high energetic and insensitive munitions compounds by Fe/Cu bimetal reduction. J Hazard Mater 219:75–81. https://doi.org/10.1016/j.jhazmat.2012.03.048
Kumar V, Sehgal R, Gupta R (2023) Microbes and wastewater treatment. In: Shah MP, Rodriguez-Couto S, Nadda AK, Daverey A (eds) Development in wastewater treatment research and processes. Elsevier, pp 239–255. https://doi.org/10.1016/B978-0-323-88505-8.00010-3
Kundu K, Landuyt VJ, Mattelin V, Martin B, Neyts M, Parmentier K, Boon N (2023) Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome. Mar Pollut Bull 190. https://doi.org/10.1016/j.marpolbul.2023.114866
Kyprianou D, Berglund M, Emma G, Rarata G, Anderson D, Diaconu G, Exarchou V (2020) Synthesis of 2,4,6-trinitrotoluene (TNT) using flow chemistry. Molecules 25:3586. https://doi.org/10.3390/molecules25163586
Lamba J, Anand S, Dutta J, Chatterjee S, Nagar S, Celin SM, Rai PK (2021) Study on aerobic degradation of 2,4,6-trinitrotoluene (TNT) using Pseudarthrobacter chlorophenolicus collected from the contaminated site. Environ Monit Assess 193(2):80. https://doi.org/10.1007/s10661-021-08869-7
Lamba J, Anand S, Dutta J, Rai PK (2022) 2,4,6-trinitrotoluene (TNT) degradation by Indiicoccus explosivorum (S5-TSA-19). Arch Microbiol 204:447. https://doi.org/10.1007/s00203-022-03057-8
Lata K, Kushwaha A, Ramanathan G (2021) Bacterial enzymatic degradation and remediation of 2,4,6-trinitrotoluene. In: Das S, Dash HR (eds) Microbial and natural macromolecules. Academic Press, pp 623–659.https://doi.org/10.1016/B978-0-12-820084-1.00024-7
Lewis TA, Ederer MM, Crawford RL, Crawford DL (1997) Microbial transformation of 2, 4, 6-trinitrotoluene. J Ind Microbiol Biotechnol 18(2):89–96. https://doi.org/10.1038/sj.jim.2900258
Li C, Li W, Ding Y, Zhou J, Ziao Z (2023) Progress and future of green explosives and propellants. Chem Energy Sour Chin Sci Bull 68(25):3311–3321. https://doi.org/10.1360/TB-2023-0203
Lynch JC, Myers KF, Brannon JM et al (2001) Effects of pH and temperature on the aqueous solubility and dissolution rate of 2,4,6-Trinitrotoluene (TNT), Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX). J Chem Eng Data 46(6):1549–1555. https://doi.org/10.1021/je0101496
Mahbub P, Nesterenko PN (2016) Application of photo degradation for remediation of cyclic nitramine and nitroaromatic explosives. Royal Soc Chem (great Britain) 6(81):77603–77621. https://doi.org/10.1039/C6RA12565D
Martin LJ, Comfort DS, Shea JP, Drijber AR, Kokjohn AT (1997) Denitration of 2,4,6-trinitrotoluene by Pseudomonas savastanoi. Can J Microbiol 43:447–455. https://doi.org/10.1139/m97-063
Massachusetts Military Reservation (MMR) (2001) Impact area groundwater study program, chemical fact sheet–TNT, Fact Sheet 2001–05
McCormick NG, Feeherry FE, Levinson HS (1976) Microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds. Appl Environ Microbiol 31:949–958. https://doi.org/10.1128/aem.31.6.949-958.1976
McFarlan G, Sara Y (2016) 2,4,6-Trinitrotoluene degradation pathway. http://eawag-bbd.ethz.ch/tnt/tnt_map.html
Mishra V, Lal R, Srinivasan S (2001) Enzymes and operons mediating xenobiotic degradation in bacteria. Crit Rev Microbiol 27:133–166. https://doi.org/10.1080/20014091096729
Montpas S, Samson J, Langlois E, Lei J, Piché Y, Chênevert R (1997) Degradation of 2,4,6-trinitrotoluene by Serratia marcescens. Biotech Lett 19(3):291–294. https://doi.org/10.1023/A:1018326228448
Nagar S, Anand S, Chatterjee S, Rawat DC, Lamba J, Rai KR (2021) A review of toxicity and biodegradation of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) in the environment. Environ Technol Innov 23(2–3):101750. https://doi.org/10.1016/j.eti.2021.101750
Nagar S, Shaw AK, Anand S, Celin SM, Rai PK (2018) Aerobic biodegradation of HMX by Planomicrobium flavidum. 3 Biotech 8(11):45. https://doi.org/10.1007/s13205-018-1479-5
Nagar S, Shaw AK, Anand S, Celin SM, Rai PK (2020) Biodegradation of octogen and hexogen by Pelomonas aquatica strain WS2-R2A-65 under aerobic condition. Environ Technol 1–22.https://doi.org/10.1080/09593330.2020.1812731
National Center for Biotechnology Information (2023) PubChem compound summary for CID 8376, 2,4,6-trinitrotoluene. Accessed 27 July 2023
Nyanhongo GS, Aichernig N, Ortner M, Steiner W, Guebitz GM (2009) Incorporation of 2,4,6-trinitrotoluene (TNT) transforming bacteria into explosive formulations. J Hazard Mater 165(1–3):285–290. https://doi.org/10.1016/j.jhazmat.2008.09.107
Pak JW, Knoke KL, Noguera DR, Fox BG, Chambliss GH (2000) Transformation of 2,4,6-trinitrotoluene by purified xenobiotic reductase B from Pseudomonas fluorescens I-C. Appl Environ Microbiol 66(11):4742–4750. https://doi.org/10.1128/aem.66.11.4742-4750.2000
Palazzo AJ, Leggett DC (1986) Effect and disposition of TNT in a terrestrial plant. J Environ Qual 15(1):49. https://doi.org/10.2134/jeq1986.00472425001500010012x
Perlman D (1972) Advances in applied microbiology. Academic Press
Pichtel J (2012) Distribution and fate of military explosives and propellants in soil: a review. Appl Environ Soil Sci 1–33.https://doi.org/10.1155/2012/617236
Preuss A, Fimpel J, Diekert G (1993) Anaerobic transformation of 2,4,6-trinitrotoluene (TNT). Arch Microbiol 159:345–353. https://doi.org/10.1007/BF00290917
REGENESIS JaS60088. https://regenesis.com/wp-content/uploads/2020/06/CS_REG_JaS60088_3DME_ROx_ORCA_UK.pdf, @. Accessed 16 Aug 2023
Rezaei MR, Abdoli MA, Karbassi A, Baghvand A, Khalilzadeh R (2010) Bioremediation of TNT contaminated soil by composting with municipal solid wastes. Soil Sedim Contam Int J 19(4):504–514. https://doi.org/10.1080/15320383.2010.486049
Robertson, B. K., & Jjemba, P. K. (2005). Enhanced bioavailability of sorbed 2, 4, 6-trinitrotoluene (TNT) by a bacterial consortium. Chemosphere, 58(3), 263–270. https://doi.org/10.1016/j.chemosphere.2004.08.080
Sathya R, Arasu VM, Al-Dhabi AN, Vijayaraghavan P, Ilavenil S, Rejiniemon ST (2023) Towards sustainable wastewater treatment by biological methods–a challenges and advantages of recent technologies. Urban Clim 47. https://doi.org/10.1016/j.uclim.2022.101378
Schackmann A, Müller R (1991) Reduction of nitroaromatic compounds by different Pseudomonas species under aerobic conditions. Appl MicrobiolBiotechnol 34.https://doi.org/10.1007/BF00169355
Schuster R, Strehse JS, Ahvo A, Turja R, Maser E, Bickmeyer U, Lehtonen KK, Brenner M (2021) Exposure to dissolved TNT causes multilevel biological effects in Baltic mussels (Mytilus spp.) Mar Environ Res 167:105264. https://doi.org/10.1016/j.marenvres.2021.105264
Serrano-González YM, Chandra R, Castillo-Zacarias C, Robledo-Padilla F, Rostro-Alanis JM, Parra-Saldivar R (2018) Biotransformation and degradation of 2,4,6-trinitrotoluene by microbial metabolism and their interaction. Def Technol 14(2):151–164. https://doi.org/10.1016/j.dt.2018.01.004
Shin CY, Crawford DL (1995) Biodegradation of trinitrotoluene (TNT) by a strain of Clostridium bifermentans. U.S. Department of Energy Office of Scientific and Technical Information. http://www.osti.gov/scitech/biblio/474237
Singh B, Kaur J, Singh K (2012) Microbial remediation of explosive waste. Crit Rev Microbiol 38(2):152–167. https://doi.org/10.3109/1040841x
Singleton I (1994) Microbial metabolism of xenobiotics: fundamental and applied research. J Chem Tech Biotechno 59:9–23. https://doi.org/10.1002/jctb.280590104
Sinha S, Chattopadhyay P, Pan I, Chatterjee S, Chanda P, Bandyopadhyay D, Das K, Sen SK (2009) Microbial transformation of xenobiotics for environmental bioremediation. Afr J Biotech 8(22):6016. https://doi.org/10.5897/AJB09.740
Solyanikova IP, Baskunov BP, Baboshin MA, Saralov AI, Golovleva LA (2011) Detoxification of high concentrations of trinitrotoluene by bacteria. Appl Microbiol Biotechnol 48:21–27. https://doi.org/10.1134/S0003683812010152
Spain PR, Johnson J, Glenn R (2023) Bacterial degradation of DNT and TNT mixtures, CU 1212. Final technical report, 31 OCT 2005. Accessed 24 Aug 2023
Tharakan JP, Gordon JA (1999) Cometabolic biotransformation of trinitrotoluene (TNT) supported by aromatic and non-aromatic co-substrates. Chemosphere 38(6):1323–1330. https://doi.org/10.1016/s0045-6535(98)00533-5
Thenmozhi A, Devasena M (2019) Remediation of 2,4,6-trinitrotoluene persistent in the environment–a review. Soil Sediment Contam Int J 1–13. https://doi.org/10.1080/15320383.2019.1664394
Timmis KN, Pieper DH (1999) Bacteria designed for bioremediation. Trends Biotechnol 17:200–204
USEPA (2014) Technical fact sheet -2,4,6-Trinitrotoluene (TNT). Office of Solid Waste and Emergency Response, 1–8. Washington, DC, U.S.A
Van Aken B, Yoon JM, Schnoor JL (2004) Biodegradation of nitro-substituted explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5-tetrazocine by a phytosymbiotic Methylobacterium sp. associated with poplar tissues (Populus deltoides x). Appl Microbiol Biotechnol 70:508–517. https://doi.org/10.1128/AEM.70.1.508-517.2004
Vanderberg LA, Perry JJ, Unkefer PJ (1995) Catabolism of 2,4,6-trinitrotoluene by Mycobacterium vaccae. Appl Microbiol Biotechnol 43:937–945. https://doi.org/10.1007/BF02431931
Vila M, Mehier S, Lorber-Pascal S, Laurent F (2007) Phytotoxicity to and uptake of RDX by rice. Environ Pollut 145(3):813–817. https://doi.org/10.1016/j.envpol.2006.05.009.
Vorbeck C, Lenke H, Fischer P, Knackmuss HJ (1994) Identification of a hydride Meisenheimer complex as a metabolite of 2,4,6-trinitrotoluene by a Mycobacterium strain. J Bacteriol 176(3):932e-e934. https://doi.org/10.1128/jb.176.3.932-934.1994
Vorbeck C, Lenke H, Fischer P, Spain JC, Knackmuss HJ (1998) Initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene. Appl Microbiol Biotechnol 64:246–252. https://doi.org/10.1128/AEM.64.1.246-252.1998
Wallace L, Cronin MP, Day AI, Buck DP (2009) Electrochemical method applicable to treatment of wastewater from nitrotriazolone production. Environ Sci Technol 43(6):1993–1998. https://doi.org/10.1021/es8028878
Wanaratna P, Christodoulatos C, Sidhoum M (2006) Kinetics of RDX degradation by zero-valent iron (ZVI). J Hazard Mater 136(1):68–74. https://doi.org/10.1016/j.jhazmat.2005.11.015
Wang CJ, Thiele S, Bollag JM (2002) Interaction of 2,4,6-trinitrotoluene (TNT) and 4-amino-2,6-dinitrotoluene with humic monomers in the presence of oxidative enzymes. Arch Environ Contam Toxicol 42:1–8. https://doi.org/10.1007/s002440010284
Wong AJ, Miller JL, Perdue B, Janik MJ (2023) Investigating the electrocatalytic reduction of 2,4,6-tri-nitro-toluene (TNT) using density functional theory methods. Green Chem 25(13):5097–5112. https://doi.org/10.1039/D3GC01144E
Xin-feng L, Chang Y, Jing-yu W et al (2023) Research process of microbial remediation of energetic compound contaminated sites. Chin J Energ Mater 31(7):714–728. https://doi.org/10.11943/CJEM2023011
Xu M, Liu D, Sun P, Li Y, Wu M, Liu W, Maser E, Xiong G, Guo L (2021) Degradation of 2,4,6-trinitrotoluene (TNT): involvement of protocatechuate 3,4-Dioxygenase (P34O) in Buttiauxella sp. S19–1. Toxics 9:231. https://doi.org/10.3390/toxics9100231
Xu M, He L, Sun P, Wu M, Cui X, Liu D, Adomako-Bonsu AG, Geng M, Xiong G, Guo L, Maser E (2023) Critical role of monooxygenase in biodegradation of 2,4,6-trinitrotoluene by Buttiauxella sp. S19–1. Molecules 28(4):1969. https://doi.org/10.3390/molecules28041969
Ziganshin AM, Ziganshina EE, Byrne J, Gerlach R, Struve E, Biktagirov T (2015) Fe (III) mineral reduction followed by partial dissolution and reactive oxygen species generation during 2,4,6-trinitrotoluene transformation by the aerobic yeast Yarrowia lipolytica. AMB Express 5:8. https://doi.org/10.1186/s13568-014-0094-z
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Lamba, J., Bhardwaj, D., Anand, S., Dutta, J., Rai, P.K. (2024). Biodegradation of 2,4,6-Trinitrotoluene (TNT) by the Microbes and Their Synergistic Interactions. In: Bala, K., Ghosh, T., Kumar, V., Sangwan, P. (eds) Harnessing Microbial Potential for Multifarious Applications. Energy, Environment, and Sustainability. Springer, Singapore. https://doi.org/10.1007/978-981-97-1152-9_7
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