Abstract
Chickpea is an important legume, and its protein-rich seeds make it a healthy alternative to meat for humans. Furthermore, the low glycemic index of carbohydrates in the grain is considered healthy for humans. Chickpea is a cheap source of protein for the people in India, Bangladesh, Pakistan, Africa, and the Mediterranean region. Therefore, the improvement of the orphan chickpea is necessary to achieve food and nutritional security in those countries. The potential of the chickpea improvement was impeded by the green revolution, and the consequence was a slow (<1% per annum) increase in the global chickpea yield which was recorded since 1990. The lack of availability of high-yielding varieties with adequate protection from various stresses is the reason for low yield. The International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and a few other national institutions have been releasing improved varieties; however, abiotic stresses, pests, diseases, and weeds remain challenging to manage in the field and storage conditions. Water and salinity stresses are significant in Asia, Australia, and the Mediterranean regions, while Helicoverpa armigera, aphids, and Ascochyta are predominant in Asia, Australia, and Canada. In the Middle-East, weeds compete with chickpea for water and nutrition. For many of these constraints, conventional or advanced breeding approaches are limited due to the lack of resistant/tolerant sources within the gene pool. Genetic engineering has the potential to address some of these constraints; however, it needs adequate resources to achieve significant impacts. In the past decade, efforts have been made to genetically modify the chickpea genome using either Agrobacterium-mediated or biolistic method. In a few instances, success has been documented; for example, genes from Bacillus thuringiensis have been introduced for complete resistance to pod borers (Helicoverpa armigera). Also, the drought-tolerant trait has been incorporated using transgene. These traits were either tested in the greenhouse or approved for field trials; however, yet to be commercialized. The possibility to save the yield losses by genetic engineering is immense and has been successful in other legumes such as soybean, common bean, and cowpea. Thus, a second green revolution may be implemented to improve the potential of chickpea and other grain legumes to attain food and nutritional security of the growing population.
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References
Acharjee S, Sarmah BK (2013) Biotechnologically generating transgenic ‘super chickpea’ for food and nutritional security. Plant Sci 207:108–116
Acharjee S, Sarmah BK, Kumar PA, Olsen K, Mahon R, Moar WJ et al (2010) Transgenic chickpea (Cicer arietinum L.) expressing a sequence-modified cry2Aa gene. Plant Sci 178:333–339
Atkinson FS, Foster-Powell K, Brand-Miller JC (2008) International tables of glycemic index and glycemic load values: 2008. Diabetes Care 31(2):2281–2283
Bajaj YPS, Dhanju MJ (1979) Regeneration of plants from apical meristem tips of some legumes. Curr Sci 48:906–907
Barna KS, Wakhlu AK (1993) Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Rep 12:521–524
Barna KS, Wakhlu AK (1994) Whole plant regeneration of Cicer arietinum from callus cultures via organogenesis. Plant Cell Rep 13:510–513
Bhatnagar-Mathur P, Rao JS, Vadez V, Sharma KK (2009) Transgenic strategies for improved drought tolerance in legumes of semi-arid tropics. J Crop Improv 24:92–111
Bhowmik SSD, Cheng AY, Long H, Tan GZH, Hoang TML, Karbaschi MR, Williams B, Higgins TJV, Mundree SG (2019) Robust genetic transformation system to obtain non-chimeric transgenic chickpea. Front Plant Sci 26:510–524
Brookes G, Barfoot P (2017) Environmental impacts of genetically modified (GM) crop use 1996–2015: impacts on pesticide use and carbon emissions. GM Crops Food 8:117–147
Chakraborti D, Sarkar A, Mondal HA, Das S (2009) Tissue specific expression of Allium sativum leaf agglutinin (ASAL) in important pulse crop chickpea (Cicer arietinum L.) to resist the phloem feeding Aphis craccivora. Transgenic Res 18:529–544
Chakraborty J, Sen S, Ghosh P, Sengupta A, Basu D, Das S (2016) Homologous promoter derived constitutive and chloroplast targeted expression of synthetic cry1Ac in transgenic chickpea confers resistance against Helicoverpa armigera. Plant Cell Tissue Org Cult 125:521–535
Chandra R, Chatrath A, Polisetty R, Khetarpal S (1993) Differentiation of in vitro grown explants of chickpea (Cicer arietinum L.) Ind J Plant Physiol 36:121–124
Chauhan R, Tiwari A, Singh NP (2003) Differential requirement of mature and immature embryo of chickpea (Cicer arietinum L.) for in vitro regeneration. Ind J Plant Physiol 8:28–33
Clemente TE, Cahoon EB (2009) Soybean oil: genetic approaches for modification of functionality and total content. Plant Physiol 151:1030–1040
Dhingra S (1994) Development of resistance in the bean aphid Aphis craccivora Koch to various insecticides used for nearly a quarter-century. Entomol Res 18:105–108
Dias CAR, Yadav TD (1988) Incidence of pulse beetles in different legume seeds. Indian J Entomol 50:457–461
Dutta I, Saha P, Majumder P, Sarkar A, Chakraborty D et al (2005a) The efficacy of a novel insecticidal protein, Allium sativum leaf lectin (ASAL), against homopteran insects monitored in transgenic tobacco. Plant Biotechnol 3:601–611
Dutta I, Majumder P, Saha P, Ray K, Das S (2005b) Constitutive and phloem-specific expression of Allium sativum leaf agglutinin (ASAL) to engineer aphid (Lipaphis erysimi) resistance in transgenic Indian mustard (Brassica juncea). Plant Sci 169:996–1007
FAO (2017) FAOSTAT, Food and Agriculture Organization, FAO statistical year book. http://www.faostat.fao.org
Fontana GS, Santini L, Caretto S, Frugis G, Mariotti D (1993) Genetic transformation in the grain legume Cicer arietinum L. (chickpea). Plant Cell Rep 12:194–198
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Ganguly M, Molla KA, Karmakar S, Datta K, Datta SK (2014) Development of pod borer-resistant transgenic chickpea using a pod-specific and a constitutive promoter-driven fused cry1Ab/Ac gene. Theor Appl Genet 127:2555–2565
Gaur PM, Samineni S, Sajja S, Chibbar RN (2015) Achievements and challenges in improving nutritional quality of chickpea. Legume Perspect 9:31–33
Giri AP, Harsulkar AM, Deshpande DV, Sainani MN, Gupta VS, Ranjekar PK (1998) Chickpea defensive proteinase inhibitors can be inactivated by pod borers gut proteinases. Plant Physiol 116:393–401
Gujar GT, Kumari A, Kalia V, Chandrashekar K (2000) Spatial and temporal variation in susceptibility of the American bollworm Helicoverpa armigera (Hubner) to Bacillus thuringiensis var. kurstaki in India. Curr Sci 78:995–1000
Hajyzadeh M, Turktas M, Khawar KM, Unver T (2015) miR408 overexpression causes increased drought tolerance in chickpea. Gene 555(2):186–193
Hariri G, Tahhan O (1983) Updating results on evaluation of the major insects which infest faba bean, lentil and chickpea in Syria. Arab J Plant Prod 1:13–21
Hazarika N, Acharjee S, Boruah RR, Babar K, Parimi S, Char B, Armstrong J, Moore A, Higgins TJV, Sarmah BK (2019) Enhanced expression of Arabidopsis Rubisco small subunit gene promoter regulated Cry1Ac gene in chickpea conferred complete resistance to Helicoverpa armigera. J Plant Biochem Biotechnol. https://doi.org/10.1007/s13562-019-00531-1
Hilder VA, Powell KS, Gatehouse AMR, Gatehouse JA, Gatehouse LN, Shi Y, Hamilton WDO, Merryweather A, Newell CA, Timans JC (1995) Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids. Transgenic Res 4:18–25
ISAAA (2018) Global status of commercialized biotech/GM crops in 2018: biotech crop adoption surges as economic benefits accumulate in 22 years. ISAAA brief no. 53. ISAAA, Ithaca
ISAAA (2019) Nigeria approves first GM food crop for open cultivation. http://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=17196
Ishimoto M, Sato T, Chrispeels MJ, Kitamura K (1996) Bruchid resistance of transgenic azuki bean expressing seed α–amylase inhibitor of common bean. Entomol Exp Appl 79:309–315
Jayanand B, Sudarsanam G, Sharma KK (2003) An efficient protocol for the regeneration of whole plants of chickpea (Cicer arietinum L.) by using axillary meristem explants derived from in vitro germinated seedlings. In Vitro Cell Dev Biol Plant 39:171–179
Jukanti AK, Gaur PM, Gowda CLL, Chibbar RN (2012) Nutritional quality and health benefits of chickpea (Cicer arietinum): a review. Br J Nutr 108:S11–S26
Jurat-Fuentes JL, Adang MJG (2001) Importance of Cry1 d-endotoxin domain II loops for binding specificity in Heliothis virescens (L.). Appl Environ Microbiol 67:323–329
Kar S, Johnson TM, Nayak P, Sen SK (1996) Efficient transgenic plant regeneration through Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.). Plant Cell Rep 16:32–37
Kar S, Basu D, Das S, Ramakrishanan NA, Mukherjee P, Nayak P et al (1997) Expression of cry1Ac gene of Bacillus thuringiensis in transgenic chickpea plants inhibits development of borer (Heliothis armigera) larvae. Transgenic Res 15:473–497
Kashiwagi J, Krishnamurthy L, Purushothaman R, Upadhyaya HD, Gaur PM, Gowda CLL et al (2015) Scope for improvement of yield under drought through the root traits in chickpea (Cicer arietinum L.). Field Crops Res 170:47–54
Krishnamurthy KV, Suhasini K, Sagare AP, Meixner M, de Kathen A, Pickardt T, Schieder O (2000) Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) embryo axes. Plant Cell Rep 19:235–240
Lee RY, Reiner D, Dekan G, Moore AE, Higgins TJ, Epstein MM (2013) Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. PLoS One 8(1):e52972. https://doi.org/10.1371/journal.pone.0052972
Malik KA, Saxena PK (1992) Thidiazuron induces high frequency shoots regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris). Aust J Plant Physiol 19:731–740
Mehrotra M, Singh AK, Sanyal I et al (2011) Pyramiding of modified cry1Ab and cry1Ac genes of Bacillus thuringiensis in transgenic chickpea (Cicer arietinum L.) for improved resistance to pod borer insect Helicoverpa armigera. Euphytica 182:87
Mudryj AN, Yu N, Aukema HM (2013) Nutritional and health benefits of pulses. Appl Physiol Nutr Metab 39:1197–1204
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Neelam A, Reddy CS, Reddy GM (1986) Multiple shoots and plantlet regeneration from shoot apex and hypocotyl explants of C. arietinum L. Int Chickpea Newsl 14:13–16
Paul V, Chandra R, Khetarpal S, Polisetty R (2000) Effect of BAP induction period on shoot differentiation from seeding explants of chickpea (Cicer arietinum L.). J. Plant Biol 27:235–239
Polisetty R, Patil P, Deveshwar JJ, Khetarpal S, Chandra R (1996) Rooting and establishment of in vitro shoot tip explants of chickpea (Cicer arietinum L.). Ind J Exp Biol 34:806–809
Polisetty R, Patil P, Deveshwar JJ, Khetarpal S, Suresh K, Chandra R (1997) Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). Plant Cell Rep 16:565–571
Polowick PL, Baliski DS, Mahon JD (2004) Agrobacterium tumefaciens-mediated transformation of chickpea (Cicer arietinum L.): gene integration, expression and inheritance. Plant Cell Rep 23:485–491
Prakash S, Chowdhary JB, Jain RK, Chowdhary VK (1992) Factors affecting plant regeneration in chickpea, Cicer arietinumL. Ind J Exp Biol 30:1149–1153
Prescott VE, Campbell PM, Moore A, Mattes J, Rothenberg ME et al (2005) Transgenic expression of bean alpha-amylase inhibitor in peas results in altered structure and immunogenicity. J Agric Food Chem 53:9023–9030
Rao KV, Rathore KS, Hodges TK, Fu X, Stoger E, Sudhakar D et al (1998) Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper. Plant J 15:469–477
Reckling M, Bergkvist G, Watson CA, Stoddard FL et al (2016) Trade-offs between economic and environmental impacts of introducing legumes into cropping systems. Front Plant Sci 7:1–14
Reddy SV, Kumar PL (2004) Transmission and properties of a new luteovirus associated with chickpea stunt disease in India. Curr Sci 86:1157–1161
Rizvi SMH, Singh RP (2000) In vitro plant regeneration from immature leaflet-derived callus cultures of Cicer arietinum L. via organogenesis. Plant Cell Biotechnol Mol Biol 1:109–114
Rizvi SMH, Jaiwal PK, Singh RP (2002) A possible involvement of cellular polyamine level in thidiazuron induced somatic embryogenesis in chickpea. In: Nandi SK, Palni LMS, Kumar A (eds) Role of plant tissue culture in biodiversity conservation economic development. Gyanodaya Prakashan, Nainital, pp 163–175
Sadeghi A, Smagghe G, Broeders S, Hernalsteens JP, De Greve H et al (2008) Ectopically expressed leaf and bulb lectins from garlic (Allium sativum L) protect transgenic tobacco plants against cotton leaf worm (Spodoptera littoralis). Transgenic Res 17:9–18
Saha P, Chakraborti D, Sarkar A, Dutta I, Basu D et al (2007) Characterization of vascular-specific RSs1 and rolC promoters for their utilization in engineering plants to develop resistance against hemipteran insect pests. Planta 226:429–442
Sandberg AS (2002) Bioavailability of minerals in legumes. Br J Nutr 88(S3):281–285
Sanyal I, Singh AK, Kaushik M, Amla DV (2005) Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) with Bacillus thuringiensis cry1Ac gene for resistance against pod borer insect Helicoverpa armigera. Plant Sci 168:1135–1146
Sarmah BK, Moore A, Tate W, Molvig L, Morton RL et al (2004) Transgenic chickpea seeds expressing high levels of a bean α-amylase inhibitor. Mol Breed 14:73–82
Schroeder HE, Gollasch S, Moore A, Tabe LM, Craig S, Hardie D, Chrispeels MJ, Spencer D, Higgins TJV (1995) Bean α-amylase inhibitor confers resistance to the pea weevil, Bruchus pisorum, in genetically engineered peas (Pisum sativum L.). Plant Physiol 107:1233–1239
Shade RE, Schroeder HE, Pueyo JJ, Tabe LM, Murdock LL, TJV H, Chrispeels MJ (1994) Transgenic pea seeds expressing the a-amylase inhibitor of the common bean are resistant to bruchid beetles. Biotechnology 12:793–796
Sharma HC (2001) Cotton bollworm/legume pod borer, Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera): biology and management. Crop Protection Compendium. Commonwealth Agricultural Bureau International, Oxon, 72p
Shri PV, Davis TM (1992) Zeatin-induced shoot regeneration from immature chickpea (Cicer arietinum L.) cotyledons. Plant Cell Tissue Organ Cult 28:45–51
Singh KB (1997) Chickpea (Cicer arietinum L.). Field Crops Res 53:161–170
Somers DA, Samac DA, Olhoft PM (2003) Recent advances in legume transformation. Plant Physiol 131:892–899
Srivastava AK, Dixit GP, Singh NP (2017) Assessing chickpea yield gaps in India: a tale of two decades. Outlook Agric 1:1–6
Tripathi L, Singh AK, Singh S, Sing R, Chaudhary S, Sanyal I, Amla DV (2013) Optimizzation of regeneration and Agrobacterium-mediated transformation of immature chickpea (Cicer arietnum). Plant Cell Tissue Organ Cult 113:513–527
United Nation (2017) The world population prospects: the 2017 revision. The UN Department of Economic and Social Affairs. https://www.un.org/development/desa/en/news/population/world-population-prospects-2017.html
Vani AKS, Reddy VD (1996) Morphogenesis from callus cultures of chickpea, (Cicer arietinum L.). Ind J Exp Biol 34:285–289
Varshney RK, Hiremath PJ, Lekha P, Kashiwagi J, Balaji J, Deokar AA et al (2009) A comprehensive resource of drought- and salinity- responsive ESTs for gene discovery and marker development in chickpea (Cicer arietinum L.). BMC Genom 10:523. https://doi.org/10.1186/1471-2164-10-523
Varshney RK, Thudi M, Roorkiwal M, He W et al (2019) Resequencing of 429 chickpea accessions from 45 countries provides insights into genome diversity, domestication and agronomic traits. Nat Genet 5:857–864
Wallace TC, Murray R, Zelman KM (2016) The nutritional value and health benefits of chickpea and hummus. Nutrients 8(12):766–776
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Acharjee, S. (2021). Genetically Engineered Chickpea: Potential of an Orphan Legume to Achieve Food and Nutritional Security by 2050. In: Kavi Kishor, P.B., Rajam, M.V., Pullaiah, T. (eds) Genetically Modified Crops. Springer, Singapore. https://doi.org/10.1007/978-981-15-5897-9_6
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