Skip to main content
SpringerLink
Log in

The cell biology of pollen development in Brassica

  • Chapter
Genetic control of self-incompatibility and reproductive development in flowering plants

Part of the book series: Advances in Cellular and Molecular Biology of Plants ((CMBP,volume 2))

Abstract

Several aspects of cell and molecular biology of Brassica have been largely developed because this genus plays a central role in crop improvement (see list of cultivars of Brassica oleracea in Table 1). In addition to these applied aspects, Brassica oleracea and to a lesser degree, B. campestris, are among the best models to carry molecular investigations on sporophytic self-incompatibility (review in Nasrallah et al. 1991).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abbott, A.G. Ainsworth, C.C. and Flavell, R.B. (1984) Characterization of anther differentiation in cytoplasmic male sterile maize using a specific isozyme system (esterase). Theor. Appl. Genet. 67: 469–473.

    CAS  Google Scholar 

  • Albani, D. Robert, L.S., Donaldson, P.A., Altosaar, I., Arnison, P.G. and Fabijanski, S.F. (1990) Characterization of a pollen-specific gene family from Brassica napus which is activated during early microspore development. Plant Mol. Biol. 15: 605–622.

    Article  PubMed  CAS  Google Scholar 

  • BAP Reports (1990) Biotechnology R and D in the EC Biotechnology Action Programme, Part II. Detailed Final Report of BAP contractors in Risk Assessment (1985–1990). I. Economidis (ed.). EC publ., Bruxelles, 137 pp.

    Google Scholar 

  • Bateman, A.J. (1955) Self-incompatibility systems in Angiosperms III. Cruciferae. Heredity 9: 53–68.

    Article  Google Scholar 

  • Bedinger P.A. and Edgerton, M.D. (1990) Developmental staging of maize microspores reveals a transition in developing microspore proteins. Plant Physiol. 92: 474–479.

    Article  PubMed  CAS  Google Scholar 

  • Brown, S.M. and Crouch, M.L, (1990) Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase. Plant Cell 2: 263–274.

    PubMed  CAS  Google Scholar 

  • Chaboud, A. and Perez, R. (1992) Generative cells and male gametes: isolation, physiology and biochemistry. Int. Rev. Cytol. 140: 205–232.

    Article  CAS  Google Scholar 

  • Charzynska, M., Ciampolini, F. and Cresti, M. (1988) Generative cell division and sperm cell formation in barley. Sex. Plant Reprod. 1: 240–247.

    Article  Google Scholar 

  • Charzynska, M., Murgia, M., Milanesi, C., and Cresti, M. (1989a) Origin of sperm cell association in the `male germ unit’ of Brassica pollen. Protoplasma 149: 1–4.

    Article  Google Scholar 

  • Charzynska, M., Murgia, M. and Cresti, M. (1989b) Ultrastructure of the vegetative cell of Brassica napus pollen with particular reference to microbodies. Protoplasma 152: 22–28.

    Article  Google Scholar 

  • Clegg, J.S., Seitz, P., Seitz, N. and Hazelwood, C.F. (1982) Cellular responses to extreme water loss: the water replacement hypothesis. Cryobiology 19: 306–316.

    Article  PubMed  CAS  Google Scholar 

  • Delvallée, I. and Dumas, C. (1988) Anther development in Zea mays. Changes in protein, peroxydase and esterase patterns. J. Plant Physiol. 132: 210–217.

    Article  Google Scholar 

  • Detchepare, S., Heizmann, P. and Dumas, C. (1989) Changes in protein patterns and protein synthesis during anther development in Brassica oleracea. J. Plant Physiol. 135: 129–137.

    Article  CAS  Google Scholar 

  • Ducker, S. and Knox, R.B. (1985) Pollen and pollination: A historical review. Taxon 34: 401–419.

    Article  Google Scholar 

  • Dumas, C. and Gaude, T. (1982) Stigma-pollen recognition and pollen hydration. Phytomorphology 31: 191–201.

    Google Scholar 

  • Dumas, C. and Gaude, T. (1993) Progress in fertilization in Angiosperms. In: R.J. Jordan (ed.), The Molecular Biology of Flowering, pp. 185–218. C.A.B. International, Wallingford, Oxon, UK.

    Google Scholar 

  • Dumas, C. and Russell, S.D. (1992) Plant reproductive biology: trends. Int. Rev. Cytol. 140: 565–592.

    Article  Google Scholar 

  • Dumas, C., Duplan, J.C., Said, C. and Soulier, J.P. (1983) 1H Nuclear Magnetic Resonance to correlate water content and pollen viability. In: D.L. Mulcahy and E. Ottaviano (eds.), Pollen: Biology and Implications for Plant Breeding, pp. 15–20. Elsevier Biomedical, New York.

    Google Scholar 

  • Dumas, C. Knox, R.B. and Gaude, T. (1984a) Pollen-pistil recognition: New concepts from electron microscopy and cytochemistry. Int. Rev. Cytol. 90: 239–242.

    Article  CAS  Google Scholar 

  • Dumas, C., Knox, R.B., McConchie, C.A. and Russell, S.D. (1984b) Emerging physiological concepts in fertilization. What’s new. Plant Physiol. 15: 17–20.

    Google Scholar 

  • Dumas, C., Knox, R.B. and Gaude, T. (1985) The spatial association of the sperm cells and vegetative nucleus in the pollen grain of Brassica. Protoplasma 124: 168–174.

    Article  Google Scholar 

  • Dunwell, J.M. and Paul, E.M. (1990) Impact of genetically modified crops in agriculture. Outlook Agric. 19: 103–109.

    Google Scholar 

  • Dupuis, I. and Dumas, C. (1990) Influence of temperature stress on in vitro fertilization and heat shock protein in maize (Zea mays L.) reproductive tissue. Plant Physiol. 94: 665–670.

    Article  PubMed  CAS  Google Scholar 

  • Dzelzkalns, V.A., Nasrallah, J.B. and Nasrallah, M.E. (1992) Cell-cell communication in plants: selfincompatibility in flower development. Dev. Biol. 153: 70–82.

    Google Scholar 

  • Ebert, P.R., Anderson, M.A., Bernatzky, R., Altschuler, M. and Clarke, A.E. (1989) Genetic polymorphism of self incompatibility in flowering plants. Cell 56: 255–262.

    Article  PubMed  CAS  Google Scholar 

  • Elleman, C.J. and Dickinson, H.G. (1986) Pollen-stigma interactions in Brassica. IV. Structural reorganization in the pollen grains during hydration. J. Cell Sci. 80: 141–157.

    PubMed  CAS  Google Scholar 

  • Frova, C. (1990) Analysis of gene expression in microspores, pollen, and silks of Zea mays L. Sex. Plant Reprod. 3, 200–203.

    Article  Google Scholar 

  • Frova, C., Binelli, G. and Ottaviano, E. (1987) Isozyme and HSP gene expression during male gametophyte development in Maize. In: M.C. Rattazi, J.G. Scandalios and G.S. Whitt (eds.), Genetics, Development and Evolution, vol. 15, Isozymes: Current Topics in Biological and Medical Research, pp. 97–120. Alan R. Liss Inc., New York.

    Google Scholar 

  • Gaude, T. and Dumas, C. (1986) Organization of stigma surface components in Brassica: a cytochemical study. J. Cell Sci. 82: 203–216.

    PubMed  CAS  Google Scholar 

  • Gaude, T. and Dumas, C. (1987) Molecular and cellular events of self-incompatibility. Int. Rev. Cytol. 107: 333–366.

    Article  Google Scholar 

  • Gaude, T., Fumex, B. and Dumas, C. (1983) Are lectin-like compounds involved in stigma pollen adhesion and/or recognition in Populus and Brassica? In: D.L. Mulcahy and E. Ottaviano (eds.), Pollen: Biology and Implications for Plant Breeding, pp. 265–272. Elsevier Biomedical, New York.

    Google Scholar 

  • Gaude, T., Denoroy, L. and Dumas, C. (1991) Use of a fast protein electrophoretic purification procedure for N-terminal sequence analysis to identify S-locus related proteins in stigmas of Brassica oleracea. Electrophoresis 12: 646–653.

    Article  PubMed  CAS  Google Scholar 

  • Gaude, T., Friry, A., Heizmann, P., Mariac, C., Rougier, M., Fobis, I. and Dumas, C. (1993) Expression of a self-incompatibility gene in a self-compatible line of Brassica oleracea. Plant Cell 5: 75–86.

    PubMed  CAS  Google Scholar 

  • Gay, G., Kerhoas, C. and Dumas, C. (1986) Micro-isoelectric focusing on single pollen grain in Cucurbita pepo. Electrophoresis 7: 148–149.

    Article  CAS  Google Scholar 

  • Gay, G. Kerhoas, C. and Dumas, C. (1987) Pollen gene expression analysed by micro-isoelectric focusing of proteins from isolated pollen grains in Cucurbita pepo L. Theor. Appl. Genet. 6: 497–503.

    Google Scholar 

  • Grant, I., Beversdorf, W.D. and Peterson, R.L. (1986) A comparative light and electron microscopic study of microspore and tapetal development in male fertile and cytoplasmic male sterile oilseed rape (Brassica napus). Can. J. Bot. 64: 1055–1068.

    Article  Google Scholar 

  • Guilluy, C.M., Trick, M., Heizmann, P. and Dumas, C. (1991) PCR detection of transcripts homologous to the self incompatibility gene in anthers of Brassica. Theor. Appt. Genet. 82: 466–472.

    Article  CAS  Google Scholar 

  • Hanson, D.D., Hamilton, D.A., Travis, J.L., Bashe, D.M. and Mascarenhas, J.P. (1989) Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1: 173–179.

    PubMed  CAS  Google Scholar 

  • Heizmann, P., Friry, A., Guilluy, C.M. and Dumas, C. (1991) Expression of SLG self-incompatibility genes in anthers of Brassica oleracea: New members in the SLG gene family. Plant Sci. 80: 193–199.

    Article  Google Scholar 

  • Heslop-Harrison, J. (1968) Pollen wall development. Science 161: 230–237.

    Article  PubMed  CAS  Google Scholar 

  • Heslop-Harrison, J. (1979) An interpretation of the hydrodynamics of pollen. Am. J. Bot. 66: 737–743.

    Article  Google Scholar 

  • Heslop-Harrison, J. and Heslop-Harrison, Y. (1970) Evaluation of pollen viability by enzymatically induced fluorescence: intracellular hydrolysis of fluorescein diacetate. Stain Technol. 45: 115–120.

    PubMed  CAS  Google Scholar 

  • Hoekstra, F.A., Crowe, J.H. and Crowe, L.W. (1991) Effect of sucrose on phase behavior of membranes in intact pollen of Typha latifolia L., as measured with Fourier transform infrared spectroscopy. Plant Physiol. 97: 1073–1079.

    Article  PubMed  CAS  Google Scholar 

  • Jensen, W.A. (1973) Fertilization in flowering plants. Bioscience 23: 21–27.

    Article  Google Scholar 

  • Kerhoas, C. and Dumas, C. (1986) Nuclear Magnetic Resonance and pollen quality. In: H.F. Linskens and J.F. Jackson (eds.), Nuclear Magnetic Resonance. Modern Methods of Plant Analysis. New ser., vol. 2, pp. 169–190. Springer-Verlag, New York.

    Chapter  Google Scholar 

  • Kerhoas, C., Gay, G. and Dumas, C. (1987) A multidisciplinary approach to the study of the plasma membrane in Zea mays pollen during a controlled dehydration. Planta 171: 1–10.

    Article  Google Scholar 

  • Kirch, H.H., Kaufmann, K., Wemmer, K., Liu, J.L. and Thompson, R.D. (1992) Molecular analysis of gametophyte of self-incompatibility. In: Y. Dattée, C. Dumas and A. Gallais (eds.), Reproductive Biology and Plant Breeding, pp. 163–176. Springer-Verlag, New York.

    Chapter  Google Scholar 

  • Knox, R.B. (1984) Pollen-pistil interactions. In: H.F. Linskens and J. Heslop-Harrison (eds)., Encyclopedia of Plant Physiology, New ser., vol. 17, Cellular Interactions, pp. 508–608. Springer-Verlag, Berlin.

    Google Scholar 

  • Knox, R.B. Williams, E. and Dumas, C. (1986) Pollen, pistil and Reproductive function in crop plants. In: J. Janick (ed.), Plant Breeding Review, vol. 4, pp. 8–79. The Avi Publ. Co., Westport, CT.

    Google Scholar 

  • Lardon, A., Triboi-Blondel, A.M. and Dumas, C. (1993) A model for studying pollination and pod development in Brassica napus: The culture of isolated flowers. Sex. Plant Reprod. 6: 52–56.

    Article  Google Scholar 

  • Lewis, D. (1960) Genetic control of specificity and activity of the S antigen in plants. Proc. R. Soc. Lond. Ser. B. 151: 468–477.

    Article  CAS  Google Scholar 

  • Linskens, H.F. (1966) Die Änderung des Protein-und Enzym-Musters während der Pollen Meiose und Pollen Entwicklung. Planta 69: 79–91.

    Article  CAS  Google Scholar 

  • Mandaron, P., Niogret, M.F., Mache, R. and Moneger, F. (1990) In vitro protein synthesis in isolated microspores of Zea mays at several stages of development. Theor. Appl. Genet. 80: 134–138.

    Article  CAS  Google Scholar 

  • Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J. and Goldberg, R.B. (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347: 737–741.

    Article  CAS  Google Scholar 

  • Mascarenhas, J.P. (1990) Gene activity during pollen development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41: 317–338.

    Article  Google Scholar 

  • Matthys-Rochon, E., Vergne, P., Detchepare, S. and Dumas, C. (1987) Male germ unit isolation from three tricellular pollen species: Brassica oleracea, Zea mays, and Triticum aestivum. Plant Physiol. 83: 464–466.

    Article  PubMed  CAS  Google Scholar 

  • McConchie, C.A., Russell, S.D., Dumas, C., Tuohy, M. and Knox, R.B. (1987) Quantitative cytology of the sperm cells of Brassica campestris and B. oleracea. Planta 170: 446–452.

    Article  Google Scholar 

  • McCormick, S. (1991) Molecular analysis of male gametogenesis in plants. Trends Genet. 81: 298–303.

    Google Scholar 

  • Mogensen, H.L. (1992) The male germ unit: Concept, composition and significance. Int. Rev. Cytol. 140: 129–147.

    Article  Google Scholar 

  • Mulcahy, D.L. (1986) Gametophytic gene expression. In: A.D. Blonstein and P.J. King (eds.), A genetic Approach to Plant Biochemistry: Plant Biochemical Genetics, vol. 1, pp. 247–258. Springer-Verlag, Wien.

    Chapter  Google Scholar 

  • Murgia, M., Charzynska, M., Rougier, M. and Cresti, M. (1991a) Secretory tapetum of Brassica oleracea L.: polarity and ultrastructural features. Sex. Plant Reprod. 4: 28–35.

    Google Scholar 

  • Murgia, M., Detchepare, S., Van Went, J.L. and Cresti, M. (1991b) Brassica napus pollen development during generative cell and sperm cell formation. Sex. Plant Reprod. 4: 176–181.

    Google Scholar 

  • Nasrallah, J.B., Nishio, T. and Nasrallah, M.E. (1991) The self-incompatibility genes of Brassica: expression and use in genetic ablation of floral tissues. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 393–422.

    Article  Google Scholar 

  • Nave, E.B. and Sawhney, V.K. (1986) Enzymatic changes in post-meiotic anther development in Petunia hybrida. I. Anther ontogeny and isozyme analysis. J. Plant Physiol. 125: 451–465.

    Article  CAS  Google Scholar 

  • Palevitz, B.A. and Tiezzi, A. (1992) Organization, composition, and function of the generative cell and sperm cytoskeleton. Int. Rev. Cytol. 140: 149–185.

    Article  Google Scholar 

  • Pandey, K.K. (1960) Evolution of gametophytic and sporophytic systems of self-incompatibility in angiosperms. Evolution 14: 98–115.

    Article  Google Scholar 

  • Paul, E. and Dunwell, J. (1990) Implications of field observations for the development of biosafety protocols for small scale trials of genetically modified plants. In: E.C. Reports Brussels, Proc. USDA Symp. on Biosafety Results of Field Tests, U.S.A., November, 1990.

    Google Scholar 

  • Pierson, E.S. and Cresti, M. (1992) Cytoskeleton and cytoplasmic organization of pollen and pollen tubes. Int. Rev. Cytol. 140: 73–125.

    Article  CAS  Google Scholar 

  • Priestley, D.A. and De Kruiff, B. (1982) Phospholipid motional characteristics in a dry biological system. A 31-P nuclear magnetic resonance study of hydrating Typha latifolia pollen. Plant Physiol. 70: 1075–1078.

    Article  PubMed  CAS  Google Scholar 

  • Raghavan, V. (1984) Protein synthetic activity during normal pollen development and during induced pollen embryogenesis in Hyoscyamus niger. Can. J. Bot. 62: 2493–2513.

    Google Scholar 

  • Roberts, I.N., Gaude, T., Harrod, G. and Dickinson, H.G. (1983) Pollen-stigma interactions in Brassica oleracea: a new germination medium and its use in elucidating the mechanism of self-incompatibility. Theor. Appl. Genet. 65: 231–238.

    Article  Google Scholar 

  • Roberts, M.R., Robson, F., Foster, G.D., Draper, J. and Scott, R.J. (1991) A Brassica napus mRNA expressed specifically in developing microspores. Plant Mol. Biol. 17: 295–299.

    Article  PubMed  CAS  Google Scholar 

  • Roeckel, P., Heizmann, P., Dubois, M. and Dumas, C. (1988) Attempts to transform Zea mays via pollen grains. Effect of pollen and stigma nuclease activities. Sex. Plant Reprod. 1: 156–163.

    Article  Google Scholar 

  • Roeckel, P., Chaboud, A. Matthys-Rochon, E., Russell, S.D. and Dumas, C. (1990) Sperm cell structure, development and organization. In: S. Blackmore and R.B. Knox (eds.), Microspores. Evolution and Ontogeny. pp. 281–308. Academic Press, New York.

    Google Scholar 

  • Russell, S.D. (1985) Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm. Proc. Natl. Acad. Sci. U.S.A. 82: 6129–6131.

    Google Scholar 

  • Russell, S.D. and Dumas, C. (1992) Sexual Reproduction in Flowering Plants. Academic Press, New York.

    Google Scholar 

  • Russell, S.D., Cresti, M. and Dumas, C. (1990) Recent progress on sperm characterization in flowering plants. Physiol. Plant. 80: 669–676.

    Article  Google Scholar 

  • Sarker, R.H., Elleman, C.J. and Dickinson, H.G. (1988) Control of pollen hydration in Brassica requires continued protein synthesis, and glycosylation is necessary for intraspecific incompatibility. Proc. Natl. Acad. Sci. U.S.A. 85: 4340–4344.

    Article  PubMed  CAS  Google Scholar 

  • Sato, T., Thorness, M. K., Kandasamy, M.K., Nishio, M., Nasrallah, J.B. and Nasrallah, M.E. (1991) Activity of an S locus gene promoter in pistils and anthers of transgenic Brassica. Plant Cell 3: 867–875.

    PubMed  CAS  Google Scholar 

  • Saunders, L.C. and Lord, E. (1989) Direct movement of latex particles in the gynoecia of three species of flowering plants. Science 243: 1606–1608.

    Article  Google Scholar 

  • Saunders, L.C., Wang, C.-S. and Lord, E. (1991) Homolog of the substrate adhesion molecule vitronectin occurs in four species of flowering plants. Plant Cell 3: 629–635.

    Google Scholar 

  • Schrauwen, J.A.M., De Groot, P.F.M., Van Herpen, M.M.A., Van der Lee, T., Reynen, W.H., Weterings, K.A.P. and Wullemes, G.J. (1990). Stage-related expression of mRNAs during pollen development in lily and tobacco. Planta 182: 298–304.

    Article  CAS  Google Scholar 

  • Scott, R.J., Dagless, E., Hodge, R., Paul, W., Soufleri, I. and Draper, J. (1991) Patterns of gene expression in developing anthers of Brassica napus. Plant Mol. Biol. 17: 195–207.

    CAS  Google Scholar 

  • Singh, A. and Kao, T. (1992) Gametophytic self-incompatibility: biochemical, molecular genetic, and evolutionary aspects. Int. Rev. Cytol. 140: 449–484.

    Article  PubMed  CAS  Google Scholar 

  • Singh, M.B., O’Neill, P.M. and Knox, R.B. (1985) Initiation of post-meiotic ß galactosidase synthesis during microsporogenesis in oil seed rape. Plant Physiol. 77: 225–228.

    Article  PubMed  CAS  Google Scholar 

  • Southworth, D. (1990) Exine biochemistry. In: S. Blackmore and R.B. Knox (eds.), Microspores. Evolution and Ontogeny, pp. 193–212. Academic Press, New York.

    Google Scholar 

  • Stein, J.C., Howlett, B., Boyes, D.C., Nasrallah, M.E. and Nasrallah, J.B. (1991) Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc. Natl. Acad. Sci. U.S.A. 88: 8816–8820.

    Article  PubMed  CAS  Google Scholar 

  • Stinson, J.R., Eisenberg, A.J., Willing, R.P., Pe, M.E., Hanson, D.D. and Mascarenhas, J.P. (1987) Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol. 83: 442–447.

    Article  PubMed  CAS  Google Scholar 

  • Theunis, C.H., Pierson, E.S. and Cresti, M. (1991) Isolation of male and female gametes in higher plants. Sex. Plant Reprod. 4: 145–154.

    Article  Google Scholar 

  • Thorsness, M.K., Kandasamy, M.K., Nasrallah, M.E. and Nasrallah, J.B. (1991) The Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana. Dev. Biol. 143: 173–184.

    Article  PubMed  CAS  Google Scholar 

  • Toriyama, K., Thorsness, M.K., Nasrallah, J.B. and Nasrallah, M.E. (1991) A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis. Dev. Biol. 143: 427–431.

    Article  PubMed  CAS  Google Scholar 

  • Tupy, J., Suss, J., Hrabetova, E. and Rihova, L. (1983) Developmental changes in gene expression during pollen differentiation and maturation in Nicotiana tabacum L. Biol. Plant. 25: 231–237.

    Article  Google Scholar 

  • Twell, D., Wing, R., Yamagushi, J. and MacCormick, S. (1989) Isolation and expression of an anther-specific gene from tomato. Mol. Gen. Genet. 247: 240–245.

    Article  Google Scholar 

  • Ursin, V.M., Yamagushi, J. and MacCormick, S. (1989) Gametophytic and sporophytic expression of anther-specific genes in developing tomato anthers. Plant Cell 1: 727–736.

    PubMed  CAS  Google Scholar 

  • Vergne, P. and Dumas, C. (1988) Isolation of viable wheat gametophytes of different stages of development and variations in their protein patterns. Plant Physiol. 88: 969–972.

    Article  PubMed  CAS  Google Scholar 

  • Vergne, P., Delvallée, I. and Dumas, C. (1987) Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization. Stain Technol. 62: 299–304.

    PubMed  CAS  Google Scholar 

  • Villanueva, V.R., Mathivet, V. and Sangwan, R.S. (1985) RNA, proteins and polyamines during gametophytic and androgenetic development of pollen in Nicotiana tabacum and Datura innoxia. Plant Growth Regulation 3: 293–307.

    Article  CAS  Google Scholar 

  • Walker, J.C. and Zhang, R. (1990) Relationship of a putative kinase from maize to the S-locus glycoproteins of Brassica. Nature (London) 345: 743–746.

    Article  CAS  Google Scholar 

  • Willing, R.P. and Mascarenhas, J.P. (1984) Analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol. 75: 865–868.

    Article  PubMed  CAS  Google Scholar 

  • Willing, R.P., Basche, D. and Mascarenhas, J.P. (1988) An analysis of the quantity and diversity of messenger RNAs from pollen and shoots of Zea mays. Theor. Appl. Genet. 75: 751–753.

    Article  CAS  Google Scholar 

  • Wing, R.A., Yamaguchi, J., Larabell, S.K., Ursin, V.M. and McCormick, S. (1989) Molecular and genetic characterization of two pollen expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia. Plant Mol. Biol. 14: 17–28.

    Article  Google Scholar 

  • Wu, F.S. and Murry, L.E. (1985) Changes in protein and amino acid content during anther development in fertile and cytoplasmic male sterile Petunia. Theor. Appl. Genet. 71: 68–73.

    Article  CAS  Google Scholar 

  • Zarsky, V., Capkova, V., Hrabetova, E. and Tupy, J. (1985) Protein changes during pollen development in Nicotiana tabacum L. Biol. Plant. 27: 438–444.

    Article  CAS  Google Scholar 

  • Zenkteler, M. (1990) In vitro fertilization of ovules of some species of Brassicaceae. Plant Breeding 105: 221–228.

    Article  Google Scholar 

  • Zenkteler, M., Maheswaran, G. and Williams, E.G. (1987) In vitro placental pollination in Brassica campestris and B. napus. J. Plant Physiol. 128: 245–250.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ENS-Lyon, UMR CNRS-INRA, 46 Allée d’Italie, F-69364, Lyon, France

    Christian Dumas, Thierry Gaude, Philippe Heizmann & Mireille Rougier

Authors
  1. Christian Dumas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thierry Gaude
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Philippe Heizmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mireille Rougier
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Division of Horticulture, CSIRO, G.P.O. Box 350, 5001, Adelaide, SA, Australia

    Elizabeth G. Williams

  2. School of Botany, University of Melbourne, 3052, Parkville, Victoria, Australia

    Adrienne E. Clarke  & R. Bruce Knox  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Dumas, C., Gaude, T., Heizmann, P., Rougier, M. (1994). The cell biology of pollen development in Brassica . In: Williams, E.G., Clarke, A.E., Knox, R.B. (eds) Genetic control of self-incompatibility and reproductive development in flowering plants. Advances in Cellular and Molecular Biology of Plants, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1669-7_15

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-1669-7_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4340-5

  • Online ISBN: 978-94-017-1669-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation