Abstract
Homogeneous populations of developing microspores and pollen from anthers of lily (Lilium longiflorum Thumb.) and tobacco (Nicotiana tabacum L.) show a continuous production of biomass, reaching a maximum in young pollen. The rate of RNA synthesis was 460 fg · h−1 in young binucleate cells, 138 fg · h−1 in late binucleate cells and 56 fg · h−1 in microspores. The mRNA population in developing pollen can be separated into three groups. In the first group, certain types of mRNAs are present at a constant level during all stages of development. A second group is characteristic of young pollen and increases quantitatively until anthesis. A third group is seen transiently; to this belong mRNAs present only before mitosis or at a distinct cell stage after mitosis. Some of the translation products of this latter group of mRNAs showed similarities between lily and tobacco on two-dimensional gels in respect of molecular weight and isolectric point, indicating that those mRNAs and proteins play a role in the regulation of pollen development.
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Abbreviations
- cDNA:
-
copy DNA
- pI:
-
isolectric point
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Schrauwen, J.A.M., de Groot, P.F.M., van Herpen, M.M.A. et al. Stage-related expression of mRNAs during pollen development in lily and tobacco. Planta 182, 298–304 (1990). https://doi.org/10.1007/BF00197125
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DOI: https://doi.org/10.1007/BF00197125