Abstract
The I3 cDNA isolated from a library made from 2–4 mm (immature) anthers of Brassica napus shows microspore-specific expression. Homologous transcripts are detected in buds and anthers of male-fertile plants, but not in green tissues, roots, or in cytoplasmic male-sterile buds. High expression of the transcript is limited to microspores entering and undergoing mitosis. The predicted peptide sequence of the cDNA shows an unusual repeated alanine/proline motif at the C-terminus, which may be of importance in the native protein structure.
References
Albani D, Robert LS, Donaldson PA, Altosaar PG, Arnison PG, Fabijanski SF: Characterisation of a pollen-specific gene family from Brassisca napus which is activated during early microspore development. Plant Mol Biol 15: 605–622 (1990).
Cassab GI, Varner JE: Cell wall proteins. Annu Rev Plant Physiol Plant Mol Biol 39: 321–353 (1988).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Feinberg AP, Vogelstein B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267 (1984).
Hanson DD, Hamilton DA, Travis JI, Bashe DM, Mascarenhas JP: Characterisation of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1: 173–179 (1989).
Joshi CP: An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl Acids Res 15: 6643–6653 (1987).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Mascarenhas JP: The male gametophyte of flowering plants. Plant Cell 1: 657–664 (1989).
Mascarenhas JP: Gene activity during pollen development. Annu Rev Plant Physiol Plant Mol Biol 41: 317–338 (1990).
Messing J, Vieira J: A new pair of M13 vectors for selecting either DNA strand of double digest restriction fragments. Gene 19: 269–276 (1982).
Nevins JR: The pathway of eukaryotic mRNA formation. Annu Rev Biochem 52: 441–446 (1983).
Oguru H: Studies on the new male sterility in Japanese radish with special reference to utilisation of sterility towards the practical raising of hybrid seed. Mem Fac Agric Kagoshima Univ 6: 39–78 (1968).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Scott RS, Dagless E, Hodge R, Paul W, Soufleri I, Draper J: The identification of anther-specific genes in Brassica napus. Plant Mol Biol, in press (1991).
van Tunen AJ, Mur LA, Brouns GS, Rienstra JD, Koes RE, Mol JNM: Pollen- and anther-specific chi promoters from petunia: Tandem promoter regulation of the chiA gene. Plant Cell 2: 393–401 (1990).
Wing RA, Yamaguchi J, Larabell SK, Ursin VM, Mc-Cormick S: Molecular and genetic characterisation of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinid. Plant Mol Biol 14: 17–28 (1989).
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Roberts, M.R., Robson, F., Foster, G.D. et al. A Brassica napus mRNA expressed specifically in developing microspores. Plant Mol Biol 17, 295–299 (1991). https://doi.org/10.1007/BF00039509
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DOI: https://doi.org/10.1007/BF00039509