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Actual, potential and speculative applications of seaweed cellular biotechnology: some specific comments on Gelidium

  • Conference paper
International Workshop on Gelidium

Part of the book series: Developments in Hydrobiology ((DIHY,volume 68))

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Abstract

Cellular biotechnology is a promising application in the propagation and selection of superior strains of seaweeds. Although axenic cultures, organogenetic tissue cultures, vegetative micro-propogation, callus induction and high yields of agar from calli have been described for several species of Gelidium, a number of basic problems remain to be solved. These include standardized methods for obtaining axenic cultures, identification of requirements for organic nutrients, PGR’s, cellular disorganization and reorganization, somaclonal variation and somatic incompatibilities. Future progress in seaweed biotechnology will depend on the resolution of many of these problems.

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References

  • Apt, K. E., 1988. Etiology and development of hyperplasia induced by Streblonema sp. (Phaeophyta) on members of the Laminariales (Phaeophyta). J. Phycol. 24: 28–34.

    Article  Google Scholar 

  • Araki, T., T. Aoki & M. Kitamikado, 1987. Preparation and regeneration of protoplasts from wild-type of Porphyra yezoensis and green variant of P. cernera. Bull. Jpn. Soc. Sci. Fish. 53: 1623–1627.

    Article  CAS  Google Scholar 

  • Ausich, R. L., 1983. Method for introducing foreign genes into green algae utilizing T-DNA of agrobacterium. European patent application no. 83306603.8. Publication no. 0108580. Applied for by Standard Oil Company, 200 East Randolph Drive, Chicago, IL 60601.

    Google Scholar 

  • Benitez, L. V. & J. M. Macaranas, 1979. Partial purification of a carrageenase from the tropical sea urchin Diadema setosum. Proc. Int. Seaweed Symp. 9: 353–359.

    Google Scholar 

  • Bingham, S. E. & J. A. Shiff, 1973. Conditions for attachment of single cells released from mechanically disrupted thalli of Prasiola stipitata. Biol. Bull. 154: 425.

    Google Scholar 

  • Björk, M., P. Ekman, A. Wallin & M. Pedersén, 1990. Effects of growth rate and other factors on protoplast yield from four species of the red seaweed Gracilaria (Rhodophyta). Bot. mar. 33: 433–439.

    Article  Google Scholar 

  • Bonneau, E. R., 1976. Variation in growth and morphology of Ulva in axenic culture. Thesis, Connecticut. University, Gorton CT, 155 p.

    Google Scholar 

  • Boyen, C., B. Kloareg, M. Polne-Fuller & A. Gibor, 1990. Preparation of alginate lyases from marine molluscs for protoplast isolation in brown algae. Phycologia 29: 173–181.

    Article  Google Scholar 

  • Bradley, P. M. & D. P. Cheney, 1986. Morphogenetic variation in tissue cultures of a red seaweed. Plant Physiol. Suppl. 80: 129.

    Google Scholar 

  • Bryhni, E., 1978. Quantitative differences between poly-saccharide compositions in normal differentiated Ulva mutabilis and the undifferentiated mutant lumpy. Phycologia 17: 119–124.

    Article  CAS  Google Scholar 

  • Butler, D. M. & L. V. Evans, 1988. Isolation of protoplasts from Laminaria. Br. Phycol. J. 23: 284.

    Google Scholar 

  • Butler, D. M., K. Ostgaard, C. Boyen, L. V. Evans, A. Jensen & B. Kloareg, 1989. Isolation conditions for high yields of protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae). J. exp. Bot. 40: 1237–1246.

    Article  CAS  Google Scholar 

  • Cantacuzene, A., 1930. Contribution à l’étude des tumeurs bactériennes chez les Algues marines. Thése présentée à la Faculté des Sciences de l’Université de Paris, Paris, 97 p.

    Google Scholar 

  • Chauvat, F., J. Labarre, F. Ferino, P. Thuriaux & P. Fromageot, 1988. Gene transfer to the cyanobacterium Synechocystis PCC 6803. In Stadler T., J. Mollion, M.-C. Verdus, Y. Karamanos & M. H. Christiaen (eds), Algal Biotechnology. Elsevier. pp. 89–99.

    Google Scholar 

  • Chen, L. C.-M. & A. R. A. Taylor, 1978. Medullary tissue culture of the red alga Chondrus crispus. Can. J. Bot. 56: 883–886.

    Article  CAS  Google Scholar 

  • Chen, L. C.-M., 1986. Cell development of Porphyra leucosticta in culture. Bot. mar. 30: 399–403.

    Google Scholar 

  • Chen, L. C.-M., 1987. Protoplast morphogenesis of Porphyra leucosticta in culture. Bot. mar. 30: 399–403.

    Article  Google Scholar 

  • Chen, L. C.-M., M. F. Hong & J. S. Craigie, 1988. Protoplasts development from Porphyra linearis - an edible marine red alga. In Puite K. J., J. J. M. Dons, H. J. Huizing, A. J. Kool, M. Koornneef & F. A. Krens (eds), Progress in Plant Protoplast Research. Kluwer Academic Publishers. 123–124.

    Google Scholar 

  • Chen, L. C.-M., 1989. Cell suspension culture from Porphyra linearis (Rhodophyta) a multicellular marine red alga. J. appl. Phycol. 1: 153–159.

    Article  Google Scholar 

  • Cheney, D. P., 1986. Genetic engineering in seaweeds: appli-cations and current status. Nova Hedwigia 81: 22–29.

    Google Scholar 

  • Cheney, D. P., E. Mar, N. Saga & J. van der Meer, 1986. Protoplast isolation and cell division in the agar-producing seaweed Gracilaria (Rhodophyta). J. Phycol. 22: 238–243.

    Google Scholar 

  • Cheney, D. P., A. H. Luistro & P. M. Bradley, 1987. Carrageenan analysis of tissue cultures and whole plants of Agardhiella subulata. Hydrobiologia 151/152: 161–166.

    Article  Google Scholar 

  • Cheney, D. P., 1989. Interspecific ‘protoplast fusion and somatic hybridization in the. agarophyte Gracilaria. Abstracts XIII Int. Seaweed Symp. Vancouver, p. 17–69.

    Google Scholar 

  • Dai, J., 1987. The effects of digestive enzymes of five species of marine shellfish on the isolation of Porphyra yezoensis cells. Trans. Oceanol. Limnol 1:84–88.

    Google Scholar 

  • D’Amato, F., 1978. Chromosome number variation in cultured cells and regenerated plants. In Thorpe T. A. (ed.), Frontiers of Plant Tissue Culture Calgary, 288–296.

    Google Scholar 

  • Davison, I. R. & M. Polne-Fuller, 1990. Photosynthesis in protoplasts ofMacrocystispyrifera (Phaeophyta). J. Phycol. 26: 384–387.

    Article  CAS  Google Scholar 

  • Deretic, V., J. F. Gill & A. M. Chakrabarty, 1987. Alginate biosynthesis: a model system for gene regulation and function in Pseudomonas. Biotechnology 5: 469–477.

    Article  CAS  Google Scholar 

  • Ducreux, G., P. Maillard & D. Sihachard, 1988. Patterns of differentiation and regeneration in single cells and protoplasts of Sphacelaria (Phaeophyceae). In Stadler T., J. Mollion, M.-C. Verdus, Y. Karamanos & M. H. Christiaen (eds), Algal Biotechnology. Elsevier, 129–137.

    Google Scholar 

  • Ducreux, G. & B. Kloareg, 1988. Plant regeneration from protoplasts of Sphacelaria (Phaeophyceae). Planta 174: 25–29.

    Article  Google Scholar 

  • Duffield, E. C. S., S. D. Waaland & R. Cleland, 1972. Morphogenesis in the red alga Griffthsia pacifica: regeneration from single cells. Planta 105: 185–195.

    Article  Google Scholar 

  • Elyakova, L. A. & V. V. Favorov, 1974. Isolation and certain properties of alginate lyase VI from the mollusk Littorina sp. Biochimica et Biophysica Acta 358: 341–354.

    Article  CAS  Google Scholar 

  • Evans, D. A., W. R. Sharp & H. P. Medina-Felho, 1984. Somaclonal and gametoclonal variation. Am. J. Bot. 71: 759–774.

    Article  Google Scholar 

  • Fang, T. C., T. Chi-Hsun, O. Yu-Lin, T. Chin-Chin & C. Ten-Chin, 1979. Some genetic observations on the monoploid breeding of Laminaria japonica. Oceanic Selections 2: 1–12.

    Google Scholar 

  • Fang, T. C., 1983. A summary of the genetic studies of Laminaria japonica in China. In Tseng C. K. (ed.) Proceedings of the joint China-USA. Phycology Symposium. Science Press. Beijing, 123–136.

    Google Scholar 

  • Fang, T. C., Z. Yan & Z. Wang, 1983. Some preliminary observations on tissue cultures in Laminaria japonica and Undaria pinnatifida. Kezue Tougbao Sci. Bull. Jpn. 28: 247–249.

    Google Scholar 

  • Fang, T. C., 1984. Some genetic features revealed from culturing the haploid cells of kelps. Hydrobiologia 116/117: 317–318.

    Article  Google Scholar 

  • Featonby-Smith, B. C. & J. van Staden, 1984. Identification and seasonal variation of endogenous cytokinins in Ecklonia maxima. Bot. mar. 27: 527–531.

    Article  CAS  Google Scholar 

  • Fisher, D. D. & A. Gibor, 1987. Production of protoplasts from the brown alga, Sargassum muticum (Yendo) Fensholt (Phaeophyta). Phycologia 26: 488–495.

    Article  Google Scholar 

  • Fowke, L. C., P. M. Gresshoff & M. J. Marchant, 1979. Transfer of organelles of the alga Chlamydomonas reinhardii into carrot cells by protoplast fusion. Planta 144: 341–347.

    Article  Google Scholar 

  • Fries, L., 1963. On the cultivation of axenic red algae. Physiol. Plant. 16: 695–708.

    Article  CAS  Google Scholar 

  • Fries, L., 1973. Requirements for organic substances in seaweeds. Bot. mar. 26: 19–31.

    Google Scholar 

  • Fries, L., 1977. Growth regulating effects of phenylacetic acid and p-hydroxyphenylacetic acid on Fucus spiralis in axenic culture. Phycologia 16: 451–455.

    Article  CAS  Google Scholar 

  • Fries, L., 1980. Axenic tissue cultures from the sporophytes of Laminaria digitata and L. hyperborea. J. Phycol. 16: 475–477.

    Article  Google Scholar 

  • Fries, L., 1984. D-vitamins and their precursors as growth regulators in axenically cultivated marine macroalgae. J. Phycol. 20: 62–66.

    Article  CAS  Google Scholar 

  • Fujimura, T. & T. Kajiwara, 1989. Production of bioflavor by regeneration from protoplasts of Ulva pertusa. Abstracts XIII Int. Seaweed Symp. Vancouver, 17–68.

    Google Scholar 

  • Fujita, Y. & S. Migita, 1985. Isolation and culture of protoplasts from some seaweeds. Bull. Fac. Fish. 57: 39–45.

    Google Scholar 

  • Fujita, Y. & S. Migita, 1987. Fusion of protoplasts from thalli of two different color types in Porphyra yezoensis Ueda and development of fusion products. Jpn. J. Phycol. 35: 201–208.

    Google Scholar 

  • Fujita, Y. & M. Saito, 1989. Protoplast isolation and fusion in Porphyra. Abstracts XIII Int. Seaweed Symp. Vancouver, 17–68.

    Google Scholar 

  • Gabriel, M., 1970. Formation, growth, and regeneration of protoplasts of the green alga, Uronema gigas. Protoplasma 70: 135–138.

    Article  Google Scholar 

  • Galli, D. R. & A. C. Giese, 1959. Carbohydrate digestion in a herbivorous snail, Tegula funebralis. J. exp. Zool. 140: 415–439.

    Article  PubMed  CAS  Google Scholar 

  • García-Reina, G., R. Robaina & A. Luque, 1988a. Attempts to establish axenic cultures and photoautotrophic growth of Gelidium versicolor Gracilaria ferox and Laurencia sp. In Stadler T., J. Mollion, M.-C. Verdus, Y. Karamanos & M. H. Christiaen (eds), Algal Biotechnology. Elsevier. 111–118.

    Google Scholar 

  • García-Reina, G., R. Romero & A. Luque, 1988b. Regeneration of thalliclones from Laurencia. In Pais M. S. S., F. Mavituna & J. M. Novais (eds), Plant Cell Biotechnology. NATO ASI Series. Springer-Verlag. 81–86.

    Google Scholar 

  • Gautheret, R. J., 1985. History of plant tissue and cell culture: a personal account. In Vasil I. K. (ed.), Cell Culture and Somatic Cell Genetics of Plants. Academic Press. 2–60.

    Google Scholar 

  • Goff, L. J. & A. W. Coleman, 1984. Transfer of nuclei from a parasite to its host. Proc. natl. Acad. Sci. USA 81: 5420–5424.

    Article  PubMed  CAS  Google Scholar 

  • Gómez-Pinchetti, J. L., R. Robaina & G. García-Reina, 1989. Quantification of the agarolytic activity from marine herbivores. Its potential use for protoplast isolation. Abstracts XIII Int. Seaweed Symp. Vancouver, 17–97.

    Google Scholar 

  • Gusev, M. W., A. H. Tambiev, N. N. Kirikova, N. N. Shelyastina & R. R. Aslanyan, 1987. Callus formation in seven species of agarophyte marine algae. Mar. Biol. 95: 593–597.

    Google Scholar 

  • Han, H. & Y. Hongyuan, 1986. Haploids of Higher Plants in vitro. Springer Verlag. 211 pp.

    Google Scholar 

  • Kapraun, D. F., 1987. Marine biologist clones seaweed cells. Gen. Engineering News 7: 42–43.

    Google Scholar 

  • Kapraun, D. F. & S. G. Sherman, 1989. Strain selection and cell isolation of Ulvaria oxysperma (Chlorophyta) for net cultivation. Hydrobiologia 179: 53–60.

    Article  CAS  Google Scholar 

  • Karp, A., 1989. Can genetic instability be controlled in plant tissue cultures? Newsletter IAPTC, 58: 2–11.

    Google Scholar 

  • Kawashima, Y. & H. Tokuda, 1989. Callus formation of Eklonia cava. Abstracts XIIIth Int. Seaweed Symp. Vancouver, 17–27.

    Google Scholar 

  • Kloareg, B. & R. S. Quatrano, 1987a. Enzymatic removal of the cell walls from zygotes of Fucus distichus (L.) Powell (Phaeophyta). Hydrobiologia 151/152: 123–129.

    Article  Google Scholar 

  • Kloareg, B. & R. S. Quatrano, 1987b. Isolation of protoplasts from zygotes of Fucus distichus (L.) Powell. Plant Science 50: 189–194.

    Article  CAS  Google Scholar 

  • Kloareg, B., D. Kropf, R. S. Quatrano, C. Boyen & V. Vreeland, 1988. Protoplast production, cell wall regeneration and photopolarization in zygotes ofFucus distichus (L.) Powell (Phaeophyta). In Stadler T., J. Mollion, M.-C. Verdus, Y. Karamanos & M. H. Christiaen (eds), Algal Biotechnology. Elsevier. 119–128.

    Google Scholar 

  • Kloareg, B., M. Polne-Fuller & A. Gibor, 1989. Mass production of viable protoplasts from Macrocystis pyrifera. Plant Science 62: 105–112.

    Article  Google Scholar 

  • Larkin, P. J. & W. R. Scowcroft, 1981. Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor. appl. Genetics 60: 197–214.

    Google Scholar 

  • Lawlor, H. J., J. A. McComb & M. A. Borowitzka, 1988. The development of filamentous and callus-like growth in axenic cultures of Ecklonia radiata. Proc. Int. Seaweed Symp. 12: 139–149.

    Google Scholar 

  • Lawlor, H. J., J. A. McComb & M. A. Borowitzka, 1989. Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins. J. appl. Phycol. 1: 105–112.

    Article  Google Scholar 

  • Lee, T. F., 1986. Callus development from Laminaria saccharina sporophytes derived from gametophytes of aposporous origin. Abstracts for the meeting of the American Society of Limnology and Oceanography. Phycologial Society of America. 75.

    Google Scholar 

  • Lee, Y.-K. & H. M. Tan, 1988. Interphylum protoplast fusion and genetic recombination of the algae Porphyridium cruentum and Dunaliella spp. J. gen. Microbiol. 134: 635–641.

    CAS  Google Scholar 

  • LeGall, Y., J. P. Braud & B. Kloareg, 1989. Mass production of protoplasts from Chondrus crispus Stackhouse. Abstracts XIIIth Int. Seaweed Symp. Vancouver, 17–32.

    Google Scholar 

  • Lewis, J. B., 1964. Feeding and digestion in the tropical sea urchin Diadema antillarum Philippi. Can. J. Zool. 42: 549–557.

    CAS  Google Scholar 

  • Liu, W.-X., Y.-L. Tang, X.-W. Liu & T. C. Fang, 1984. Studies on the preparation and properties of sea snail enzymes. Hydrobiologia 116/117: 319–320.

    Article  Google Scholar 

  • Liu, Q. Y., 1989. The ultrastructure of cell wall regenerated by isolated Palmaria palmata protoplasts. Abstracts XIIIth Int. Seaweed Symp. Vancouver, 17–32.

    Google Scholar 

  • Liu, X.-W. & M. E. Gordon, 1987. Tissue and cell culture of New Zealand Pterocladia and Porphyra sp. Hydrobiologia 151/152: 147–154.

    Google Scholar 

  • Liu, X.-W., C. Rochas & M. E. Gordon, 1989. Callus culture of cell wall polysacharides. Abstract XIIIth. Int. Seaweed Symposium. Vancouver, 17–32.

    Google Scholar 

  • Luqin, P., Z. Fei, G. Ma, J. Zhou & Y. Zhu, 1988. A preliminary study on the raising of seedlings of Gelidium pacificum by regeneration of thallus fragments. J. Zhejiang Coll. Fisheries 7: 100–105.

    Google Scholar 

  • Matagne, R. F., R. Deltour & L. Ledoux, 1979. Somatic fusion between cell wall mutants of Chlamydomonas reinhardi. Nature 278: 344–346.

    Article  Google Scholar 

  • McLean, R. O. & D. J. Connolly, 1989. Callus and suspension culture in the Laminariales. Abstracts XIIIth Int. Seaweed Symp. Vancouver, 17–98.

    Google Scholar 

  • Millner, P. A., M. E. Callow & L. V. Evans, 1979. Preparation of protoplasts from the green alga Enteromorpha intestinalis (L.) Link Planta 147: 174–177.

    Google Scholar 

  • Misawa, M., 1977. Production of natural substances by plant cell cultures described in Japanese patents. In Barz W. (ed.), Plant Tissue Cultures and its Biotechnological Applications. Springer-Verlag. 49–101.

    Google Scholar 

  • Mooney, P. A. & J. van Staden, 1984. Lunar periodicity of the levels of endogenous cytoquinins in Sargassum heterophyllum. Bot. mar. 27: 467–472.

    CAS  Google Scholar 

  • Morrice, L. M., M. McLean, F. B. Williamson & W. F. Long, 1983. ß-Agarases from Pseudomonas atlantica. Hydro-biologia 116/117: 576–579.

    Google Scholar 

  • Mumford, T., 1987. Commercialization strategy for nori cultivation in Puget Sound, Washington. In Bird K. & P. H. Benson (eds), Seaweed Cultivation for Renewable Resources. Elsevier. 351–372.

    Google Scholar 

  • Muramatsu, T., S. Hirose & M. Katayose, 1977. Isolation and properties of alginate lyase from the mid-gut gland of wreath shell Turbo cornutus. Agric. Biol. Chem. 41: 1939–1946.

    CAS  Google Scholar 

  • Nakada, H. I. & P. C. Sweeny, 1967 Alginic acid degradation by eliminases from abalone hepatopancreas. J. Biol. Chem. 242: 845–851.

    PubMed  CAS  Google Scholar 

  • Nakamura, T., 1974. (See Misawa, 1977).

    Google Scholar 

  • Neushul, M., 1984. Marine algal tissue culture. Topical technical report for Gas Research Institute. Neushul, M. (ed.) Gas Research Institute 84/0076, Goleta CA. 46 p.

    Google Scholar 

  • Nisizawa, K., S. Fujibayashi & Y. Kashiwabara, 1968. Alginate lyases in the hepatopancreas of a marine mollusc, Dolabella auricula Solander. J. Biochem. 64: 25–37.

    PubMed  CAS  Google Scholar 

  • Ohiwa, T., 1978. Behavior of cultured fusion products from Zygnema and Spirogyra protoplasts. Protoplasma 97: 185–200.

    Article  Google Scholar 

  • Ohiwa, T., 1980. Fine structure of degenerating nuclei in intergeneric fusion products from Zygnema taceae protoplasts. Protoplasma 102: 77–95.

    Article  Google Scholar 

  • Ohiwa, T., 1981. Intergeneric fusion of Zygnema taceae protoplasts. Bot. Mag. Tokyo 94: 261–271.

    Article  Google Scholar 

  • Onishi, T., M. Suzuki & R. Kikuchi, 1985. The distribution of polysaccharide hydrolase activity in gastropods and bivalves. Bull. Jpn. Soc. Sci. Fish. 51: 301–308.

    Article  CAS  Google Scholar 

  • Pedersén, M., 1968. Ectocarpus fasciculatus: marine brown algae requiring kinetin. Nature 218: 5143.

    Article  Google Scholar 

  • Polne-Fuller, M., M. Biniaminov & A. Gibor, 1984. Vegetative propagation of Porphyra petforata. Hydrobiologia 116/117: 308–313.

    Article  Google Scholar 

  • Polne-Fuller, M., N. Saga & A. Gibor, 1986. Algal cell, callus, and tissue cultures and selection of algal strains. Nova Hedwigia 83: 30–36.

    Google Scholar 

  • Polne-Fuller, M. & A. Gibor, 1986. Calluses, cells, and protoplasts in studies towards genetic improvement of seaweeds. Aquaculture 57: 117–123.

    Article  Google Scholar 

  • Polne-Fuller, M. & A. Gibor, 1987a. Tissue culture of seaweeds. In Bird K. & P. H. Benson (eds), Seaweed cultivation for renewable resources. Elsevier. 219–239.

    Google Scholar 

  • Polne-Fuller, M. & A. Gibor, 1987b. Calluses and callus-like growth in seaweeds: Induction and culture. Hydrobiologia 151/152: 131–137.

    Article  Google Scholar 

  • Polne-Fuller, M. & A. Gibor, 1987c. Microrganisms as digestors of seaweed cell walls. Hydrobiologia 151/152: 405–409.

    Article  Google Scholar 

  • Polne-Fuller, M., 1988. The past, present, and future, of tissue culture and biotechnology of seaweeds. In Stadler T., J. Mollion, M.-C. Verdus, Y. Karamanos & M. H. Christiaen (eds), Algal Biotechnology. Elsevier. 17–31.

    Google Scholar 

  • Quatrano, R. S. & B. A. Caldwell, 1978. Isolation of a unique marine bacterium capable of growth on a wide variety of polysaccharides from macroalgae. Appl. envir. Microbiol. 36: 979–981.

    CAS  Google Scholar 

  • Reddy, C. R. K., S. Migita & Y. Fujita, 1989. Protoplast isolation and regeneration of three species of Ulva in axenic culture. Bot. mar. 32: 783–490.

    Article  Google Scholar 

  • Robaina, R., 1988. Biotecnologia del cultivo in vitro de algal rojas de interés industrial. Thesis. Universidad de Las Palmas de Gran Canaria. 236 pp.

    Google Scholar 

  • Robaina, R., G. Garcia-Reina & A. Luque, 1990a. The effects of the physical characteristic of the culture medium on the development of red seaweeds in tissue culture. Hydro-biologia (In press).

    Google Scholar 

  • Robaina, R., P. Garcia, G. García-Reina & A. Luque, 1990b. Morphogenetic effect of glycerol on tissue cultures of the red seaweed Grateloupia doryphora. J. appl. Phycol. 2: 137–143.

    Article  Google Scholar 

  • Saga, N., T. Uchida & Y. Sakai, 1978. Clone Laminaria from single isolated cell. Bull. Japan. Soc. Sci. Fish. 44: 87.

    Google Scholar 

  • Saga, N. & Y. Sakai, 1983. Axenic tissue culture and callus formation of the marine brown alga Laminaria angustata. Bull. Jpn. Soc. Sci. Fish. 49: 1561–1563.

    Article  Google Scholar 

  • Saga, N., 1984. Isolation of protoplasts from edible seaweeds. Bot. Mag. Tokyo 97: 423–427.

    Article  Google Scholar 

  • Saga, N. & Y. Sakai, 1984. Isolation of protoplasts from Laminaria and Porphyra. Bull. Japan. Soc. Sci. Fish. 50: 1085.

    Google Scholar 

  • Saga, N., M. Polne-Fuller & A. Gibor, 1986. Protoplasts from seaweeds: production and fusion. Nova Hedwigia 83: 37–43.

    Google Scholar 

  • Saga, N. & T. Kudo, 1989. Isolation and culture of protoplasts from the marine green alga Monostroma angicava. J. appl. Phycol. 1: 25–30.

    Article  Google Scholar 

  • Schiff, J. A., R. S. Quatrano, G. C. Harris, M. Legg & R. Stanley, 1972. Development of single cells released from mechanically disrupted thalli of Prasiola stipitata. Biol. Bull. 143: 476.

    Google Scholar 

  • Smith, R. G. & R. G. S. Bidwell, 1989. Inorganic carbon uptake by photosynthetically active protoplasts of the red macroalga Chondrus crispus. Mar. Biol. 102: 1–4.

    CAS  Google Scholar 

  • Sylvester, A. W. & J. R. Waaland, 1983. Cloning the red alga Gigartina exasperata for culture on artificial substrates. Aquaculture 31: 305–318.

    Article  Google Scholar 

  • Tait, M. I., A. M. Milne, D. Grant, J. A. Somers, J. Staples, W. F. Long, F. B. Williamson & S. B. Wilson, 1990. Porphyra cell cultures: isolation and polysaccharide production. J. appl. Phycol. 2: 63–70.

    Article  Google Scholar 

  • Tang, Y., 1982. Isolation and cultivation of vegetative cells and protoplasts of Porphyra suborbiculata Kjellm. J. Shandong Coll. Oceanol. 12: 49–50 (English abstract).

    Google Scholar 

  • Tatewaki, M. & K. Nagata, 1970. Surviving protoplasts in vitro and their development in Bryopsis. J. Phycol. 6: 401–403.

    Google Scholar 

  • Tokuda, H. & Y. Kawashima; 1988. Protoplast isolation and culture of brown alga Undaria pinnatifida. In Stadler T., J. Mollion, M.-C. Verdus, Y. Karamanos & M. H. Christiaen (eds), Algal Biotechnology. Elsevier. 151–157.

    Google Scholar 

  • Tsekos, I., 1982. Tumour-like growths induced by bacteria in the thallus of a red alga, Gigartina teedii (Roth) Lamour. Ann. Bot. 49: 123–126.

    Google Scholar 

  • van der Meer, J. P. & F. J. Simpson, 1984. Cryopreservation of Gracilaria tikvahiae (Rhodophyta) and other macrophytic marine algae. Phycologia. 23: 195–202.

    Article  Google Scholar 

  • van der Meer, J. P., 1986. Genetic contributions to research on seaweeds. Prog. in Phycol. Res. 4: 1–38.

    Google Scholar 

  • van der Meulen, H. J., 1975. The enzymatic hydrolysis of agar by Cytophaga flevensis sp. nov. Thesis, University of Groningen, Netherlands, 80 pp.

    Google Scholar 

  • van Rensburg, J. G. J. & B. M. Vcelar, 1989. The effect of the sucrose concentration on the initiation and growth of adventitious buds from leaf tissue of Lachenalia. S. Afr. J. Bot. 55: 117–121.

    Google Scholar 

  • Vreeland, V., E. Zablackis, B. Doboszewski & W. M. Laetsch, 1987. Molecular markers for marine algal polysaccharides. Hydrobiologia 151/152: 155–159.

    Article  Google Scholar 

  • Waaland, J. R., 1982. Cloning marine algae for mariculture. J. World. Maricul. Soc. 14: 404–414.

    Article  Google Scholar 

  • Waaland, J. R. & L. G. Dickson, 1987. Preparation of Porphyra protoplasts. J. Phycol. 23: 13.

    Article  Google Scholar 

  • Wang, S., Z. Xu, G. Wang & Z. Xia, 1986. Ultrastructural study on protoplasts of Porphyra haitanensis (Bangiophyceae, Rhodophyta). Mar. Sci. 10: 21–24 (Chinese with English abstract).

    Google Scholar 

  • Wang, S., X. Zhang, Z. Zhidong & Y. Sun, 1986. A study on the cultivation of the vegetative cells and protoplasts of Porphyra haitanensis. Oceanol. Limnol Sinica 17: 217–221.

    Google Scholar 

  • Wang, S., Y. Sun, A. Lu & G. Wang, 1987a. Early stage differentiation of thallus cells of Porphyra haitanensis. Chin. J. Oceanol. Limnol. 5: 217–224.

    Article  Google Scholar 

  • Wang, S., G. Wang, Y. Sun & A. Lu, 1987b. Isolation and cultivation of the vegetative cells of Porphyra haitanensis. Chin. J. Oceanol. Limnol 5. 333–339.

    Article  Google Scholar 

  • Wang, S. & X. Yan, 1990. Observations on the monosporelike cells in the somatic cell culture of Porphyra haitanensis. Oceanol. Limniol Sinica 21: 166–169.

    Google Scholar 

  • Wu, C. Y. & G. Lin, 1988. Progress in the genetics and breeding of economic seaweed in China. Hydrobiologia 151/152: 57–61.

    Google Scholar 

  • Yan, Z. M., 1984. Studies on tissue culture of Laminaria japonica and Undaria pinnatifida. Hydrobiologia 116: 314–316.

    Article  Google Scholar 

  • Yaphe, W., 1957. The use of agarase from Pseudomonas atlantica in the identification of agar in marine algae (Rhodophyceae). Can. J. Microbiol. 3: 987–993.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, D., 1982. Study on protoplasts preparation, culture and fusion of somatic cells from two species of green algae Ulva linza and Monostroma angicava Kjellm. J. Shandong College of Oceanol. 13: 57–65.

    Google Scholar 

  • Zhao, H. & X. Zhang, 1984. On the cultivation of the isolated vegetative cells of Porphyra yezoensis Ueda. J. Fish. China 8: 197–202.

    Google Scholar 

  • Zhu, R., 1982. A comparative study of marine algae cell wall decomposition by the digestive enzymes isolated from three species of marine gastropods. Biochem. Biophys. 2: 43–45.

    Google Scholar 

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J. A. Juanes B. Santelices J. L. McLachlan

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© 1991 Springer Science+Business Media Dordrecht

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Garcia-Reina, G., Gómez-Pinchetti, J.L., Robledo, D.R., Sosa, P. (1991). Actual, potential and speculative applications of seaweed cellular biotechnology: some specific comments on Gelidium . In: Juanes, J.A., Santelices, B., McLachlan, J.L. (eds) International Workshop on Gelidium . Developments in Hydrobiology, vol 68. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3610-5_17

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  • DOI: https://doi.org/10.1007/978-94-011-3610-5_17

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