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Isolation of protoplasts from edible seaweeds

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Abstract

Protoplasts were isolated enzymatically from three species of Chlorophyta (Enteromorpha linza, Monostroma zostericola andUlva pertusa) with high yield and viability. An enzyme solution appropriate for protoplast isolation from the marine green algae was the following: 2% Cellulase Onozuka R-10, 1.0.M mannitol, pH 6.0. Protoplasts could not be obtained from members of Phaeophyta or Rhodophyta.

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Authors and Affiliations

  1. The Institute of Algological Research, Faculty of Science, Hokkaido University, Muroran, 051, Hokkaido

    Naotsune Saga

  2. Marine Science Institute and Department of Biological Sciences, University of California, 93106, Santa Barbara, CA, USA

    Naotsune Saga

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  1. Naotsune Saga
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Saga, N. Isolation of protoplasts from edible seaweeds. Bot Mag Tokyo 97, 423–427 (1984). https://doi.org/10.1007/BF02488673

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  • DOI: https://doi.org/10.1007/BF02488673

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