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Mechanisms of T-DNA transfer and integration into plant chromosomes: role of vir B, vir D4 and vir E2 and a short interspersed repetitive element (SINE) from tobacco

  • Chapter
Molecular Mechanisms of Bacterial Virulence

Part of the book series: Developments in Plant Pathology ((DIPP,volume 3))

Abstract

The present paper describes two topics on the early events during the formation of crown gall. (1) To measure an efficiency of T-DNA transfer from Agrobacterium cells to plant nuclei, we have developed a simple procedure which relies on Agrobacterium-mediated transient expression of the intron-GUS gene in plant cells. The results of experiments by this procedure indicate that products encoded by the virB locus and by the virD4 gene are necessary for transfer of T-DNA. VirE2 protein is also required for efficient transfer of T-DNA, although it is not absolutely essential. (2) We found a new family of short interspersed repetitive element (SINE) around the T-DNA integration target sites in the tobacco genome. SINEs are one of retroposons that is thought to originate from a tRNA or its gene. The SINE designated here as the TS family is the first example of a SINE family of plant bearing the significant homology to specific tRNAs including a vertebrate tRNALys which is thought to be a cognate molecule of most animal SINEs. The TS family occurs in approximately 5.0 104 copies per tobacco genome at least. Correlation between the presence of the TS family in tobacco chromosomes and integration of T-DNA will be discussed.

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Abbreviations

SINE:

Short Interspersed Repetitive Element

AS:

Acetosyringone

GUS:

β-Glucuronidase

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Authors
  1. Yasushi Yoshioka
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  2. Yoshito Takahashi
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  3. Shogo Matsumoto
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  4. Shoko Kojima
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  5. Ken Matsuoka
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  6. Kenzo Nakamura
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  7. Kazuhiko Ohshima
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  8. Norihiro Okada
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  9. Yasunori Machida
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Editors and Affiliations

  1. University of California, Davis, CA, 95616, USA

    C. I. Kado

  2. Oregon Health Sciences University, Portland, OR, 97201, USA

    J. H. Crosa

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Yoshioka, Y. et al. (1994). Mechanisms of T-DNA transfer and integration into plant chromosomes: role of vir B, vir D4 and vir E2 and a short interspersed repetitive element (SINE) from tobacco. In: Kado, C.I., Crosa, J.H. (eds) Molecular Mechanisms of Bacterial Virulence. Developments in Plant Pathology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0746-4_17

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  • DOI: https://doi.org/10.1007/978-94-011-0746-4_17

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