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Sequence analysis of three tRNAPhe nuclear genes and a mutated gene, and one gene for tRNAAla from Arabidopsis thaliana

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Abstract

Three genes and one mutant gene for tRNAPhe (GAA) and one gene for tRNAAla (UGC) were isolated from a whole-cell DNA library of Arabidopsis thaliana. All three tRNAPhe genes are identical in their nucleotide sequence, but differ in their 5′ and 3′ flanking regions. The mutant tRNAPhe (GAA) gene differs from the other three genes by one nucleotide change from highly conserved G to C at the 57th nucleotide position. The primary structure of the first tRNAAla gene was also determined in this experiment.

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Authors and Affiliations

  1. National Institute for Basic Biology, 444, Okazaki, Japan

    Akama Kazuhito

  2. Department of Botany, Faculty of Science, Hokkaido University, 060, Sapporo, Japan

    Shigeyuki Tanifuji

Authors
  1. Akama Kazuhito
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  2. Shigeyuki Tanifuji
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Akama, K., Tanifuji, S. Sequence analysis of three tRNAPhe nuclear genes and a mutated gene, and one gene for tRNAAla from Arabidopsis thaliana . Plant Mol Biol 15, 337–346 (1990). https://doi.org/10.1007/BF00036919

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  • DOI: https://doi.org/10.1007/BF00036919

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