Abstract
In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells [45, 46]. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we determined the sequences of three 103-like cDNAs and two 103-like genes, GNT1 and GNT35. To further study the regulation of the expression of these genes their 5′ regions were translationally fused with the β-D-glucuronidase reporter gene (GUS). The GNT1 5′ region led to GUS expression only in the root tips of transgenic plants. By using transgenic hairy-root cultures and transformed cell suspension cultures it was shown that the 5′ regions of both GNT1 and GNT35 lead to 2,4-D-inducible expression of GUS activity. The homology of the 103-like genes with other auxin-regulated genes is evaluated.
Similar content being viewed by others
References
Ainley MW, Walker JC, Nagao RT, Key JL: Sequence and characterization of two auxin-regulated genes from soybean. J Biol Chem 263: 10658–10666 (1988).
Alliotte T, Tire C, Engler G, Peleman J, Caplan A, Van Montagu M, Inze D: An auxin-regulated gene of Arabidopsis thaliana encodes a DNA-binding protein. Plant Physiol 89: 743–752 (1989).
An G: High efficiency transformation of cultured tobacco cells. Plant Physiol 79: 568–570 (1985).
Barker RF, Idler KB, Thompson DV, Kemp JD: Nucleotide sequence of the T-DNA region from Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol Biol 2: 335–350 (1983).
Benton WD, Davis W: Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196: 180–182 (1977).
6. Bercetche J: PhD Thesis, Université Paris 6 (1987).
Berk AJ, Sharp PA: Sizing and mapping of early adeno-virus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12: 721–732 (1977).
Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
Birnboim HC, Doly J: A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7: 1513–1523 (1979).
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254 (1976).
Capone I, Cardarelli M, Trovato M, Costantino P: Upstream non-coding region which confers polar expression to Ri plasmid root inducing gene rolB. Mol Gen Genet 216: 239–244 (1989).
Costantino P, Hooykaas PJJ, den-Dulk-Ras H, Schilperoort RA: Tumour formation and rhizogenicity of Agrobacterium rhizogenes carrying Ti plasmids. Gene 11: 79–87 (1980).
Czarnecka E, Edelman L, Schoffl F, Key JL: Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Mol Biol 3: 45–58 (1984).
Czarnecka E, Nagao RT, Key JL: Characterization of Gmhsp26-A, a stress gene encoding a divergent heat shock protein of soybean: Heavy-metal-induced inhibition of intron processing. Mol Cell Biol 8: 1113–1122 (1988).
Ditta G, Stanfield S, Corbin D, Helinski DR: Broad host range DNA cloning system from gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347–7351 (1980).
Franco AR, Gee MA, Guilfoyle TJ: Induction and super-induction of auxin-responsive mRNAs with auxin and protein synthesis inhibitors. J Biol Chem 265: 15845–15849 (1990).
Geliebter J: Dideoxynucleotide sequencing of RNA and uncloned cDNA. Focus 9: 5–8 (1987).
Hagen G, Guilfoyle TJ: Rapid induction of selective transcription by auxins. Mol Cell Biol 5: 1197–1203 (1985).
Hagen G, Kleinschmidt A, Guilfoyle TJ: Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections. Planta 162: 147–153 (1984).
Hagen G, Uhrhammer N, Guilfoyle TJ: Regulation of expression of an auxin-induced soybean sequence by cadmium. J Biol Chem 263: 6442–6446 (1988).
Hamill JD, Prescott A, Martin C: Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes. Plant Mol Biol 9: 573–584 (1987).
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA: A binary plant vector strategy based on separating of vir and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179–180 (1983).
Hooykaas PJJ, Klapwijk PM, Nuti MP, Schilperoort RA, Rörsch A: Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent agrobacteria and to Rhizobium ex planta. J Gen Microbiol 98: 477–484 (1977).
Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT: A simple and general method for transferring genes into plants. Science 227: 1229–1231 (1985).
Jefferson RA: Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).
Jefferson RA, Kavanagh TA, Bevan M: GUS fusions: β-Glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
Joshi CP: An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl Acids Res 15: 6643–6653 (1987).
Kieny MP, Lathe R, Lecocq JP: New versatile cloning and sequencing vectors based on bacteriophage M13. Gene 26: 91–99 (1983).
Linsmaier EM, Skoog F: Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 8: 100–127 (1965).
Loenen WAM, Blattner FR: Lambda charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts. Gene 26: 171–179 (1983).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Marsh JL, Erfle M, Wykes EJ: The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481–485 (1984).
McClure BA, Guilfoyle TJ: Rapid redistribution of auxin-regulated RNAs during gravitropism. Science 243: 91–93 (1989).
McClure BA, Hagen G, Brown CS, Gee MA, Guilfoyle TJ: Transcription, organization, and sequence of an auxin-regulated gene cluster in soybean. Plant Cell 1: 229–239 (1989).
Ooms G, Hooykaas PJJ, Van Veen RJM, Van Beelen P, Regensburg-Tuïnk TJG, Schilperoort RA: Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7: 15–29 (1982).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Schmülling T, Schell J, Spena A: Promoters of the rolA, B and C genes of Agrobacterium rhizogenes are differentially regulated in transgenic plants. Plant Cell 1: 665–670 (1989).
Shaw G, Kamen R: A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46: 659–667 (1986).
Spaink HP, Okker RJH, Wijffelman CA, Pees E, Lugtenberg BJJ: Promoters in the nodulation region of the Rhizobium legiminosarum Sym plasmid pRL1JI. Plant Mol Biol 9: 29–37 (1987).
Takahashi Y, Kuroda H, Tanaka T, Machida Y, Takebe I, Nagata T: Isolation of an auxin-regulated gene cDNA expressed during the transition from Go to S phase in tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 86: 9279–9283 (1989).
Takahashi Y, Niwa Y, Machida Y, Nagata T: Location of the cis-acting auxin-responsive region in the promoter of the par gene from tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 87: 8013–8016 (1990).
Taylor TL, Fritzemeier K-H, Häuser I, Kombrink E, Rohwer F, Schröder M, Strittmatter G, Hahlbrock K: Structural analysis and activation of a gene encoding a pathogenesis-related protein in potato. Mol Plant-Microbe Interact 3: 72–77 (1990).
Teeri TH, Herrera-Estrella L, Depicker A, Van Montagu M, Palva ET: Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene. EMBO J 5: 1755–1760 (1986).
Theologis A: Rapid gene regulation by auxin. Ann Rev Plant Physiol 37: 407–438 (1986).
Van der Zaal EJ, Memelink J, Mennes AM, Quint A, Libbenga KR: Auxin-induced mRNA species in tobacco cell cultures. Plant Mol Biol 10: 145–157 (1987).
Van der Zaal EJ, Mennes AM, Libbenga KR: Auxin-induced rapid changes in translatable mRNAs in tobacco cell suspension. Planta 172: 514–519 (1987).
Van Slogteren GMS, Hoge JHC, Hooykaas PJJ, Schilperoort RA: Clonal analysis of heterogeneous crown gall tumor tissues induces by wild-type and shooter mutant strains of Agrobacterium tumefaciens-expression of T-DNA genes. Plant Mol Biol 2: 321–333 (1983).
Author information
Authors and Affiliations
Additional information
Department of Plant Molecular Biology, Leiden University
Rights and permissions
About this article
Cite this article
van der Zaal, E.J., Droog, F.N.J., Boot, C.J.M. et al. Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression. Plant Mol Biol 16, 983–998 (1991). https://doi.org/10.1007/BF00016071
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00016071