Skip to main content

Towards Sex Determination of Date Palm

  • Chapter
  • First Online:
Date Palm Biotechnology

Abstract

In the date palm (Phoenix dactylifera), a dioecious mode and late initial reproductive age of 5–10 years are major practical constraints to genetic improvement. Improvement of the existing palm cultivars or selection of new ones with superior characters is a tedious endeavor due to the long life cycle of the date palm tree and its heterozygous nature. Sexual propagation method cannot be used commercially for propagating the cultivars of interest in a true-to-type manner. Currently there is no reliable method to identify sex at the early seedling stage. Early sex identification of young seedlings could enhance breeding programs and generate experimental male and female genetic stocks that will help the genetic improvement of the date palm. Moreover, the selection and identification of superior seedling characters for yield enhancement and to improve the physical and chemical properties of fruits is of great commercial interest. There has been significant progress in our understanding of sex-determining mechanisms in date palm using traditional means. But physiological and cytological methods do not give obvious differences between male and female date palms. Biotechnology, as a new tool in date palm breeding, can be useful to improve the qualities of palm trees through early sex identification. Although molecular markers have been introduced in date palm programs, few research efforts have been geared toward studying the early sex determination in the plant. This chapter will focus on genetic and molecular basis of sex determination in date palm in attempting to develop reliable methods to identify sex at an early stage of seedlings.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Ahmed M, Soliman S, Elsayed E (2006) Molecular identification of some Egyptian date palm males by females varieties (Phoenix dactylifera L.) using DNA markers. J Appl Sci Res 2:270–275

    Google Scholar 

  • Ainsworth C (2000) Boys and girls come out to play: the molecular biology of dioecious plants. Ann Bot 86:211–221

    Article  Google Scholar 

  • Al-Khalifa NS, Khan FA, Askari E, Hadi S (2006) In vitro culture and genetic analysis of male and female date palm. Acta Hort 725:653–661

    Google Scholar 

  • Al-Salih A, Al-Najjar NR, Al-Mashdani AN (1987) A study on the chromosome number of two specific female date palm cultivars. Date Palm J 5:134–143

    Google Scholar 

  • Alstrom-Rapaport C, Lascoux M, Wang YC et al (1998) Identification of a RAPD marker linked to sex determination in the basket willow (Salix viminalis L.). J Hered 89:44–49

    Article  CAS  Google Scholar 

  • Aly M, Bacha M (2000) Chromosome characteristics of three date palm cultivars (Phoenix dactylifera L.). Sultan Qaboos Univ J Sci Res Agric Sci 5:11–14

    Google Scholar 

  • Banks JA (2008) MicroRNA, sex determination and floral meristem determinacy in maize. Genome Biol 9:204.1–204.3

    Google Scholar 

  • Beal J (1937) Cytological studies in the genus Phoenix. Bot Gaz 99:400–407

    Article  Google Scholar 

  • Bekheet S, Taha H, Hanafy M, Solliman ME (2008) Morphogenesis of sexual embryos of date palm cultured in vitro and early identification of sex type. J Appl Sci Res 4:345–352

    Google Scholar 

  • Ben-Abdallah A, Stiti K, Lepoivre P, du Jardim P (2000) Date palm (Phoenix dactylifera L.) cultivar identification using random amplified polymorphic DNA (RAPD). Cah Agric 9:103–107

    Google Scholar 

  • Bendiab K, Baaziz M, Brakez Z, Sedra MH (1993) Correlation of isoenzyme polymorphism and Bayoud-disease resistance in date palm cultivars and progeny. Euphy 65:23–32

    Article  Google Scholar 

  • Biffi R, Restivo F, Tassi F et al (1995) A restriction fragment length polymorphism probe for early diagnosis of gender in Asparagus officinalis L. HortScience 30:1463–1464

    CAS  Google Scholar 

  • Bracale M, Galli MG, Longo C et al (1990) Understanding sex differentiation in Asparagus officinalis: genetical and physiological approaches. Acta Hort 271:423–431

    Google Scholar 

  • Caporali E, Carboni P, Nicoloso L et al (1996) Sex determination and differentiation in the dioecious species Asparagus officinalis L. Plant Biosyst 130:79–81

    Google Scholar 

  • Carpenter J (1979) Breeding of date palm in California. Date Grow Inst Rep 54:13–16

    Google Scholar 

  • Chandler W (1958) Evergreen orchards. Lea & Fabiger, Philadelphia

    Google Scholar 

  • Charlesworth D (1985) Distribution of dioecy and self-incompatibility in angiosperms. In: Greenwood PJ, Slatkin M (eds) Evolution-essays in honour of John Maynard Smith. Cambridge University Press, Cambridge, pp 237–268

    Google Scholar 

  • Charlesworth D, Charlesworth B, Marais G (2005) Steps in the evolution of heteromorphic sex chromosomes. Heredity 95:118–128

    Article  PubMed  CAS  Google Scholar 

  • Dellaporta SL, Calderon-Urrea A (1993) Sex determination in flowering plants. Plant Cell 5:1241–1251

    Article  PubMed  CAS  Google Scholar 

  • DeMason D, Tisserat B (1980) The occurrence and structure of apparently bisexual flowers in the date palm, Phoenix dactylifera L. (Arecaceae). Bot J Linn Soc 81:283–292

    Article  Google Scholar 

  • DeMason D, Stolte K, Tisserat B (1982) Floral development in Phoenix dactylifera. Can J Bot 60:1437–1446

    Article  Google Scholar 

  • Deputy JC, Ming R, Ma H et al (2002) Molecular markers for sex determination in papaya (Carica papaya L.). Theor Appl Genet 106:107–111

    PubMed  CAS  Google Scholar 

  • El-Kharbotly A, El-Mardi M, Al-Saadi N, Al-Maharuki Y (1998) Towards the construction of a genetic map of date palm using the Amplified Fragment Length Polymorphism technique (AFLP). In: The first international conference on date palm. Al-Ain, March 8–10, pp 194–207

    Google Scholar 

  • FAO (1984) Production yearbook. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  • Gebhardt C, Salamini F (1992) Restriction fragment length polymorphism analysis of plant genomes and its application to plant breeding. Int Rev Cytol 135:201–237

    Article  CAS  Google Scholar 

  • Gorelick R (2003) Evolution of dioecy and sex chromosomes via methylation driving Muller’’s ratchet. Biol J Linn Soc 80:353–368

    Article  Google Scholar 

  • Gorelick R, Osborne R (2002) Inducing sex change and organogenesis from tissue culture in the endangered African cycad Encephalartos woodii (Cycadales, Zamiaceae). S Afr J Sci 98:114–117

    CAS  Google Scholar 

  • Guttman D, Charlesworth D (1998) An X-linked gene with a degenerate Y-linked homologue in a dioecious plant. Nature 393:263–266

    Article  PubMed  CAS  Google Scholar 

  • Harvey CF, Gill CP, Fraser LG, McNeilage MA (1997) Sex determination in Actinidia. 1. Sex-linked markers and progeny sex ratio in diploid A. chinensis. Sex Plant Rep 10:149–154

    Article  Google Scholar 

  • Hormaza J, Dollo L, Polito V (1994) Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis. Theor Appl Genet 89:9–13

    Article  CAS  Google Scholar 

  • Ibrahim AMF, El-Kobbia AM, Kitat FM, Abd El-Kawy NM (1998) Cytological studies on date palm. I. Chromosomal behaviour during meiosis of two date palm (Phoenix dactylifera L.) male types. Alex J Agric Res 43:237–246

    Google Scholar 

  • Komai F, Shikazono N, Tanaka A (2003) Sexual modification of female spinach seeds (Spinacia oleracea L.) by irradiation with ion particles. Plant Cell Rep 21:713–771

    PubMed  CAS  Google Scholar 

  • Liu ZY, Moore PH, Ma H et al (2004) A primitive Y chromosome in papaya marks incipient sex chromosome evolution. Nature 427:348–352

    Article  PubMed  CAS  Google Scholar 

  • Lloyd D (1975) The transmission of genes via pollen and ovules in gynodioecy angiosperms. Theor Popul Biol 9:299–316

    Article  Google Scholar 

  • Lloyd D, Bawa K (1984) Modification of the gender of seed plants in varying conditions. Evol Biol 17:255–338

    Google Scholar 

  • Ma H, Moore PH, Liu Z et al (2004) High-density linkage mapping revealed suppression of recombination at the sex determination locus in papaya. Genetics 166:419–436

    Article  PubMed  CAS  Google Scholar 

  • Mandolino G, Carboni A, Forapani S et al (1999) Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.). Theor Appl Genet 98:86–92

    Article  CAS  Google Scholar 

  • Masmoudi-Allouche F, Chaari-Rkhis A, Kriaa W et al (2009) In vitro hermaphrodism induction in date palm female flower. Plant Cell Rep 28:1–10

    Article  PubMed  CAS  Google Scholar 

  • Mason S (1915) Botanical characters of the leaves of the date palms used in distinguishing cultivated varieties, Bulletin 223:1–28. USDA, Washington, DC

    Google Scholar 

  • Mulcahy DV, Weeden NF, Kesseli RV, Carroll SB (1992) DNA probe for Y-chromosome of Silene latifolia, a dioecious angiosperm. Sex Plant Rep 3:1–3

    Google Scholar 

  • Negrutiu I, Vyskot B, Barbacar N, Moneger F (2001) Dioecious plants. A key to the early events of sex chromosome evolution. Plant Physiol 127:1418–1424

    Article  PubMed  CAS  Google Scholar 

  • Okada S, Sone T, Fujisawa M et al (2001) The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene. Proc Natl Acad Sci USA 98:9454–9459

    Article  PubMed  CAS  Google Scholar 

  • Parasnis AS, Ramakrishna W, Chowdari KV et al (1999) Microsatellite (GATA)M reveals sex-specific differences in papaya. Theor Appl Genet 99:1047–1052

    Article  CAS  Google Scholar 

  • Polley A, Seigner E, Ganal MW (1997) Identification of sex in hop (Humulus lupulus) using molecular markers. Genome 40:357–361

    Article  PubMed  CAS  Google Scholar 

  • Ponnuswami V, Jagadeesan R, Ramesh Kumar A et al (2008) Genetic relationship and diversity in palmyrah accessions based on rapid markers. Am Euras J Sust Agric 2:165–171

    Google Scholar 

  • Renner S, Ricklefs R (1995) Dioecy and its correlates in the flowering plants. Am J Bot 82:596–606

    Article  Google Scholar 

  • Restivo FM, Tassi F, Biffi R et al (1995) Linkage arrangement of RFLP loci in progenies from crosses between doubled haploid Asparagus officinalis L. clones. Theor Appl Genet 90:124–128

    Article  CAS  Google Scholar 

  • Saker M, Kuehne T (1998) RAPD marker and RAPD-based marker (SCAR) distinguish male from female papaya plants in vitro. Third Arab Conference on modern biotechnology and areas of applications in the Arab world, Cairo, 14–17 Dec 1998

    Google Scholar 

  • Saker M, Rady M (2003) Employment of molecular markers for identification of male and female papaya plants. J Gen Eng Biotechnol NRC 1:85–97

    Google Scholar 

  • Scutt CP, Gilmartin P (1998) The men-10 cDNA encodes a novel form of proline-rich protein expressed in the tapetum of dioecious Silene latifolia. Sex Plant Rep 11:236–240

    Article  CAS  Google Scholar 

  • Scutt CP, Li Y, Robertson SE, Willis ME, Gilmartin PM (1997) Sex determination in dioecious Silene latifolia (effects of the Y chromosome and the parasitic smut fungus (Ustilago violacea) on gene expression during flower development). Plant Physiol 114:969–979

    Article  PubMed  CAS  Google Scholar 

  • Shaheen M (1990) Propagation of date palm through tissue culture: a review and an interpretation. Ann Agric Sci Ain Shams Univ Cairo Egypt 35:895–909

    Google Scholar 

  • Siljak-Yakovlev S, Cerbah M, Sarr A et al (1996) Chromosomal sex determination and heterochromatin structure in date palm. Sex Plant Rep 9:127–132

    Article  Google Scholar 

  • Soliman S, Bahy A, Mohamed Morsy M (2003) Genetic comparisons of Egyptian date palm cultivars (Phoenix dactylifera L.) by RAPD-PCR. Afr J Biotechnol 2:86–87

    CAS  Google Scholar 

  • Soundur N, Manshardt R, Stiles J (1996) A genetic linkage map of papaya based on randomly amplified polymorphic DNA markers. Theor Appl Genet 93:547–553

    Article  Google Scholar 

  • Testolin R, Cipriani G, Costa G (1995) Sex segregation ratio and gender expression in the genus Actinidia. Sex Plant Rep 8:129–132

    Google Scholar 

  • Urasaki N, Tokumoto M, Tarora K et al (2002) A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.). Theor Appl Genet 104:281–285

    Article  PubMed  CAS  Google Scholar 

  • Vyskot B (1999) The role of DNA methylation in plant reproductive development. In: Ainsworth CC (ed) Sex determination in plants. BIOS Scientific Publisher, Oxford, pp 101–120

    Google Scholar 

  • Younis R, Ismail O, Soliman S (2008) Identification of sex- specific DNA markers for date palm (Phoenix dactylifera L.) using RAPD and ISSR techniques. Res J Agric Biol Sci 4:278–284

    CAS  Google Scholar 

  • Zaid A, de Wet PF (2002) Botanical and systematic description of the date palm, In: Zaid A, Arias-Jimenez EJ (eds) Date palm cultivation, Chapter 1. FAO, Rome, pp 1–47

    Google Scholar 

  • Zhang YH, DiStilio VS, Rehman F et al (1998) Y chromosome specific markers and the evolution of dioecy in the genus Silene. Genome 41:141–147

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. S. Hanafy .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media B.V.

About this chapter

Cite this chapter

Bekheet, S.A., Hanafy, M.S. (2011). Towards Sex Determination of Date Palm. In: Jain, S., Al-Khayri, J., Johnson, D. (eds) Date Palm Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1318-5_26

Download citation

Publish with us

Policies and ethics