Abstract
Next challenges in functional annotation of mammalian genomes are yet of a much larger scope than previous genomics initiatives. Mouse mutant resources must be phenotyped systematically (one after the other) and systemically (assessing all organ systems). In addition, for the next generation of mouse models the “envirotypes”, that humans are exposed to, need to be modeled. We established the German Mouse Clinic (GMC) as a mouse phenotyping platform for systemic, standardized phenotypic analysis and interpretation and open access for the scientific community on a collaborative basis. Next to phenotyping mouse models from national and international programs we provide the logistics to analyse mutant mouse lines from different origins (e.g., Knock-outs, Knock-ins, Gene-traps, RNAi Knock-downs, ENU mutagenesis, QTL studies) from collaboration partners throughout the world. Here we present an overview of how we organize a high-throughput screening of mouse lines from different laboratories and describe specific methods of selected screens in more detail.
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Notes
- 1.
Partners are the US-based Kockout Mouse Project (KOMP, http://www.knockoutmouse.org/) and Texas A&M Institute of Genomic Medicine (TIGM, http://www.tigm.org/), the European Conditional Mouse Mutagenesis (EUCOMM, http://www.eucomm.org/) and the North American Conditional Mouse Mutagenesis Project (NorCOMM, http://www.norcomm.org/) in Canada.
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Acknowledgments
We would like to thank Reinhard Seeliger, Miriam Backs, Ingrid Bayer, Nicole Boche, Sabrina Bothur, Sandra Geissler, Michaela Grandl, Tamara Halex, Elfi Holupirek, Sabine Holthaus, Constanze König, Maria Kugler, Albert Langer, Katrin Laube, Astrid Markert, Jacqueline Müller, Elenore Samson, Florian Schleicher, Daniela Schmidt, Waldemar Schneider, Ann-Elisabeth Schwarz, Bettina Sperling, Waldtraud Stettinger, Lucie Thurmann, Susanne Wittich, and Claudia Zeller as well as the GMC animal caretaker team for expert technical help. This work has been funded by the BMBF (DZD e.V. 01GI0923 and NGFNplus grant, 01GS08156, 01GS0850, 01GS0851, 01GS0852, 01GS0868, 01GS0869, 01GS0853, and 01GS0854) and by an EU grant (EUMODIC, LSHG-2006-037188, German Mouse Clinic).
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Gailus-Durner, V. et al. (2011). The German Mouse Clinic – Running an Open Access Platform. In: Brakebusch, C., Pihlajaniemi, T. (eds) Mouse as a Model Organism. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0750-4_2
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