Abstract
The technique of chromosome microdissection was initially developed for generating DNA libraries from specific parts of polytene chromosomes of Drosophila (Scalenghe 1981) in order to isolate certain genes known to be localized in those regions. First improvements of this original technique such as the use of GTG-banded chromosomes and the invention of the first sequence independent DNA amplification system (Lüdecke 1989, Senger 1990, Lüdecke 1990) allowed the generation of microdissection libraries from precisely defined regions of human chromosomes and a stepwise reduction of the number of chromosomes that had to be dissected for one library. By Trautmann and co-worker (1991) it was shown for the first time that microdissection libraries can be used as painting probes for in situ hybridization.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chudoba I, Rubtsov N, Senger G, Junker K, Bleck C, Claussen U (1996) Improved detection of chromosome 16 rearrangements in acute myeloid leukemias using 16p and 16q specific microdissection libraries. Oncol Rep 3:829–832
Lüdecke H-J, Senger G, Claussen U, Horsthemke B (1989) Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature 338:348–350
Lüdecke H-J, Senger G, Claussen U, Horsthemke B (1990) Construction and characterization of band-specific DNA libraries. Hum Genet 84:512–516
Meltzer PS, Guan X-Y, Burgess A, Trent JM (1992) Rapid generation of region specific probes by chromosome microdissection and their application. Nature Genet 1:24–28
Müller-Navia J, Nebel A, Schleiermacher E (1995) Complete and precise characterization of marker chromosomes by application of microdissection in prenatal diagnosis. Hum Genet 96:661–667
Rubtsov N, Senger G, Kuzcera H, Neumann A, Kelbova C, Junker K, Beensen V, Claussen U (1996) Interstitial deletion of chromosome 6q: precise definition of the breakpoints by microdissection, DNA amplification, and reverse painting. Hum Genet 97:705–709
Scalenghe F, Turco E, Edström JE, Pirrotta V, Melli M (1981) Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma 82:205–216
Senger G, Lüdecke H-J, Horsthemke B, Claussen U (1990) Microdissection of banded human chromosomes. Hum Genet 84:507–511
Senger G, Ragoussis J, Trowsdale J, Sheer D (1993) Fine mapping of the MHC class II region within 6p21 and evaluation of probe ordering using interphase fluorescence in situ hybridization. Cytogenet Cell Genet 64:49–53
Telenius H, Carter NP, Bebb CE, Nordenskjöld M, Ponder BAJ, Tunnacliff A (1992) Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 13:718–725
Trautmann U, Leuteritz G, Senger G, Claussen U, Ballhausen WG (1991) Detection of APC region-specific signals by nonisotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe. Hum Genet 87: 495–497
Viersbach R, Schwanitz G, Nöthen MM (1994) Delineation of marker chromosomes by reverse chromosome painting using only a small number of DOP-PCR amplified microdissected chromosomes. Hum Genet 93:663–667
Yokoyama Y, Sakuragawa N (1995) Improved simple generation of GTG-band specific painting probes. Cytogenet Cell Genet 71:32–36
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Senger, G., Weimer, J., Claussen, U., Chudoba, I. (1999). Microdissection and Reverse Chromosome Painting. In: Wegner, RD. (eds) Diagnostic Cytogenetics. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59918-7_20
Download citation
DOI: https://doi.org/10.1007/978-3-642-59918-7_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47813-0
Online ISBN: 978-3-642-59918-7
eBook Packages: Springer Book Archive